Prevalence and quantification of Listeria monocytogenes in beef offal at retail level in Selangor, Malaysia

Kuan, Chee Hao; Wong, Woan Chwen; Pui, Chai Fung; Mahyudin, Nor Ainy; Tang, John Yew Huat; Nishibuchi, Mitsuaki; Radu, Son

doi:10.1590/s1517-83822014005000002

Cited by 10 publications

(5 citation statements)

References 16 publications

(16 reference statements)

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“…On the other hand, Wang et al [30] found that only 5.5% of chilled pork samples positive for L. monocytogenes. Similar to our results, Kuan et al [31] reported the L. monocytogenes prevalence of 33.3% in beef offal from different wet markets. In turn, from the total of 50 beef meat samples examined by Teixiera et al [4], six were confirmed as L. monocytogenes positive (12%).…”

Section: Discussionsupporting

confidence: 92%

Assessment of the Prevalence and Drug Susceptibility of Listeria monocytogenes Strains Isolated from Various Types of Meat

Skowron

Wałecka-Zacharska

Wiktorczyk-Kapischke

et al. 2020

Foods

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Listeria monocytogenes are the etiological factor of listeriosis, and their main source for humans is food. The aim of the current study was to assess the contamination of various types of meat and the drug susceptibility of isolated L. monocytogenes. Between 2016–2018, 6000 swabs were taken (2000 annually) from the surface of pork, beef, and poultry. The analysis of intermediate and finished product samples was carried out in accordance with ISO 11290-1 (International Organization for Standardization). The genetic similarity assessment of the isolates obtained was based on the Pulsed Field Gel Electrophoresis (PFGE) method, and drug-sensitivity assessment using the disc-diffusion method. We found 2.1% of collected samples were L. monocytogenes positive. The level of meat contamination varied depending on its matrix. Most L. monocytogenes were isolated from poultry. It was shown that 39 (32.5%) strains were sensitive to all tested antibiotics and eight (6.7%) were resistant to all five tested antimicrobials. Most strains tested were resistant to cotrimoxazole (55; 45.8%) and meropenem (52; 43.3%), followed by erythromycin (48; 40.0%), penicillin (31; 25.8%), and ampicillin (21; 17.5%). High prevalence of this pathogen may be a serious problem, especially when linked with antibiotic resistance and high percentage of serotypes responsible for listeriosis outbreaks.

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Section: Discussionsupporting

confidence: 92%

Assessment of the Prevalence and Drug Susceptibility of Listeria monocytogenes Strains Isolated from Various Types of Meat

Skowron

Wałecka-Zacharska

Wiktorczyk-Kapischke

et al. 2020

Foods

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“…Indeed, the mPCR-MPN method has widely been used to detect and enumerate food-borne pathogens such as Campylobacter spp. (Chai et al, 2007 ), Listeria monocytogenes (Kuan et al, 2013 ) and Vibrio parahaemolyticus (Tan et al, 2017 ). Recently, gold nanoparticle-aptamer-based localized surface plasmon resonance (LSPR) sensing chip was developed to enable the ultra-sensitive and selective detection of S .…”

Section: Introductionmentioning

confidence: 99%

Prevalence, Virulence Genes and Antimicrobial Resistance Profiles of Salmonella Serovars from Retail Beef in Selangor, Malaysia

et al. 2018

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The aim of the present study was to investigate the prevalence of Salmonella spp., Salmonella Enteritidis and Salmonella Typhimurium in retail beef from different retail markets of Selangor area, as well as, to assess their pathogenic potential and antimicrobial resistance. A total of 240 retail beef meat samples (chuck = 60; rib = 60; round = 60; sirloin = 60) were randomly collected. The multiplex polymerase chain reaction (mPCR) in combination with the most probable number (MPN) method was employed to detect Salmonella spp., S. Enteritidis and S. Typhimurium in the meat samples. The prevalence of Salmonella spp., S. Enteritidis and S. Typhimurium in 240 beef meat samples were 7.50, 1.25, and 0.83%, respectively. The microbial loads of total Salmonella was found in the range of <3 to 15 MPN/g. Eight different serovars of Salmonella were identified among the 23 isolates, and S. Agona was the predominant serovar (26.09%). Interestingly, all the Salmonella isolates were resistant to penicillin, erythromycin and vancomycin, but the sensitivity was observed for tetracycline, gentamicin and amoxicillin/clavulanic acid. All 23 isolates were resistant to at least three antibiotics. Two S. Typhimurium isolates (8.70%) exhibited the highest multiple antibiotic resistance (MAR) index value of 0.56 which shown resistance to nine antibiotics. PCR analysis of virulence genes showed that all Salmonella isolates (100%) were positive for the invA gene. Meanwhile, pefA was only identified in S. Enteritidis and S. Typhimurium. The findings in this study indicate that retail beef products tested were widely contaminated with multi-drug resistant (MDR) Salmonella and various virulence genes are present among the isolated Salmonella serovars.

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“…However, L. monocytogenes prevalence was not determined for humans (samples) in all the included studies. Prevalence results for food (only) samples were determined in 15 [ 14 , [18] , [19] , [20] , [21] , [22] , [23] , [24] , [25] , [26] , [27] , [28] , [29] , [30] , [31] ] of the included studies ( Table 1 ). One study [ 15 ] had a result for the environmental (only) sample and one [ 16 ] for environmental and animal samples.…”

Section: Resultsmentioning

confidence: 99%

A systematic review and meta-analysis of the prevalence of Listeria monocytogenes in South-East Asia; a one-health approach of human-animal-food-environment

et al. 2022

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Prevalence and quantification of Listeria monocytogenes in beef offal at retail level in Selangor, Malaysia

Cited by 10 publications

References 16 publications

Assessment of the Prevalence and Drug Susceptibility of Listeria monocytogenes Strains Isolated from Various Types of Meat

Assessment of the Prevalence and Drug Susceptibility of Listeria monocytogenes Strains Isolated from Various Types of Meat

Prevalence, Virulence Genes and Antimicrobial Resistance Profiles of Salmonella Serovars from Retail Beef in Selangor, Malaysia

A systematic review and meta-analysis of the prevalence of Listeria monocytogenes in South-East Asia; a one-health approach of human-animal-food-environment

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