Molecular characterization of the first leptospires isolated from goats in Brazil

Lilenbaum, Walter; Kremer, Frederico Schmitt; Ristow, Paula; Dellagostin, Odir A.; Bourhy, Pascale; Hartskeerl, Rudy A.; Vasconcellos, Sílvio Arruda

doi:10.1590/s1517-83822014000400050

Cited by 10 publications

(9 citation statements)

References 11 publications

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“…Studies using molecular tools are scarce in Brazil, thus hindering deeper discussion on this topic. Nevertheless, occurrences of L. santarosai in different animal species in Brazil, like goats (Lilenbaum et al, 2015), dogs (Miotto et al, 2016) and cattle (Hamond et al, 2015), have been confirmed in the literature, which indicates that this genomospecies is common in this country. Detection of leptospires genetically close to L. borgpetersenii, which has only been reported in Brazil a few times, is also noteworthy.…”

Section: Discussionmentioning

confidence: 92%

Leptospira reservoirs among wildlife in Brazil: Beyond rodents

et al. 2018

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Leptospirosis is a disease of great importance in tropical regions. Infection occurs mainly through contact with water contaminated with the urine of infected animals, especially that of rodents. Despite the diversity and abundance of wild fauna in Brazil, little is known about the role of other wild species in the epidemiology of leptospirosis. This study aimed to investigate new reservoirs of Leptospira among wildlife in Brazil, using serological and molecular diagnoses in a large-sized sample. Biological samples were collected from 309 free-ranging mammals, belonging to 16 species. The majority of the animals included were opossums (Didelphis albiventris) and coatis (Nasua nasua). Blood and urine samples were subjected to the microscopic agglutination test (MAT) and real-time PCR, respectively. Genetic characterization of genomospecies was performed using PCR amplicons. Statistical analysis was applied to test associations between positive diagnoses and age, sex, season and type of environment. The prevalence of infection found via MAT and PCR was 11% and 5.5%, respectively. If these tests are taken to be complementary, the overall prevalence was 16%. The most common serogroups were Djasiman and Australis, while L. santarosai was the prevalent genomospecies. Significant differences in prevalence between animal species were observed. Greater risk of infection was detected among adult opossums than among young ones. The influence of each serogroup and genomospecies was tested for the same variables, and this revealed higher risk of infection by L. santarosai among male opossums than among females. The present study highlights the exposure and carrier status of several wild species in Brazil and it indicates that coatis and other carnivores are priorities for further investigations.

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Section: Discussionmentioning

confidence: 92%

Leptospira reservoirs among wildlife in Brazil: Beyond rodents

et al. 2018

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“…Amongst these isolates, members of serogroup Grippotyphosa are usually associated with environmental contamination and are maintained by wildlife species. From that serogroup, Bananal is a local serovar that has already been isolated in Brazil from mice, capybaras and goats (Lilenbaum et al, 2014), but had never been recovered from cattle. Serogroup Sejroe is the most common in ruminants worldwide, as well as in Brazil (Cosate et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

A multilocus variable number tandem repeat analysis assay provides high discrimination for genotyping Leptospira santarosai strains

Hamond

Pinna

Medeiros

et al. 2015

Journal of Medical Microbiology

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Considering the prevalence of Leptospira santarosai infections in the Americas and the scarce information about the species, we aimed to apply a multilocus variable number tandem repeat (VNTR) analysis (MLVA) for the molecular typing of L. santarosai isolates from various sources. Amplification of three VNTR loci selected from L. santarosai genome sequences resulted in a wide range of sizes for the amplified products amongst the 21 L. santarosai strains analysed. This suggested a variation in tandem repeat copy numbers in the VNTR loci. secY sequencing also showed a high nucleotide diversity, confirming the MLVA data. In conclusion, this novel MLVA provided a high level of discrimination between L. santarosai isolates, and this new typing tool could be used to investigate leptospirosis in regions where L. santarosai predominates.

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“…While very useful, sequencing of PCR products cannot, as yet, replace the need for bacterial culture of leptospires. The need for identification of local isolates in tropical regions has been recently discussed [9] and genome sequencing of newly identified isolates will broaden our understanding of the circulating strains.…”

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confidence: 99%

Genotyping of Leptospira directly in urine samples of cattle demonstrates a diversity of species and strains in Brazil

et al. 2015

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The aim of this study was to identify Leptospira in urine samples of cattle by direct sequencing of the secY gene. The validity of this approach was assessed using ten Leptospira strains obtained from cattle in Brazil and 77 DNA samples previously extracted from cattle urine, that were positive by PCR for the genus-specific lipL32 gene of Leptospira. Direct sequencing identified 24 (31·1%) interpretable secY sequences and these were identical to those obtained from direct DNA sequencing of the urine samples from which they were recovered. Phylogenetic analyses identified four species: L. interrogans, L. borgpetersenii, L. noguchii, and L. santarosai with the most prevalent genotypes being associated with L. borgpetersenii. While direct sequencing cannot, as yet, replace culturing of leptospires, it is a valid additional tool for epidemiological studies. An unexpected finding from this study was the genetic diversity of Leptospira infecting Brazilian cattle.

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Molecular characterization of the first leptospires isolated from goats in Brazil

Cited by 10 publications

References 11 publications

Leptospira reservoirs among wildlife in Brazil: Beyond rodents

Leptospira reservoirs among wildlife in Brazil: Beyond rodents

A multilocus variable number tandem repeat analysis assay provides high discrimination for genotyping Leptospira santarosai strains

Genotyping of Leptospira directly in urine samples of cattle demonstrates a diversity of species and strains in Brazil

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