Antimicrobial susceptibility testing for Helicobacter pylori isolates from Brazilian children and adolescents: comparing agar dilution, E-test, and disk diffusion

Ogata, Silvio Kazuo; Gales, Ana Cristina; Kawakami, Elisabete

doi:10.1590/s1517-83822014000400039

Cited by 49 publications

(42 citation statements)

References 30 publications

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“…The selection of appropriate and accurate antimicrobial susceptibility tests is important for the prescription of optimal antibiotics, the management of H. pylori treatment, the determination of patient‐specific treatment, and epidemiological resistance surveillance . E‐test is a quantitative variant of the disc‐diffusion method and is useful for slow‐growing bacteria.…”

Section: Discussionmentioning

confidence: 99%

Helicobacter pylori antimicrobial resistance in a pediatric population

Silva

Nascimento

et al. 2018

Helicobacter

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The authors found a high resistance rate of H. pylori for clarithromycin in this northern portuguese pediatric center. This factor should determine a change in local current treatment, contraindicating the use of clarithromycin as a first-line treatment for H. pylori infection in children. The high eradication rate maybe explained for the eradication treatment tailored according antimicrobial susceptibility.

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Section: Discussionmentioning

confidence: 99%

Helicobacter pylori antimicrobial resistance in a pediatric population

Silva

Nascimento

et al. 2018

Helicobacter

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“…It is reported that the results of the disk diffusion method are equivalent to those found by E-test (Mishra et al, 2006) or have a moderate correlation (Ogata et al, 2015). Moreover, the disk diffusion method is simple and easy to interpret, widely used in medical bacteriology laboratories (Jorgensen and Ferraro, 2009;Balouiri et al, 2015).…”

Section: Accepted Manuscriptmentioning

confidence: 91%

Clarithromycin resistance and prevalence of Helicobacter pylori virulent genotypes in patients from Southern México with chronic gastritis

Alarcón‐Millán

Fernández‐Tilapa

Cortés-Malagón

et al. 2016

Infection, Genetics and Evolution

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In developing countries, clarithromycin resistance and frequency of re-infection are factors that contribute to high prevalence of Helicobacter pylori infection. The aim of this research was determine the prevalence of clarithromycin resistance and its relation with A2142G, A2142C and A2143G mutations in the domain V of the 23S rRNA gene of H. pylori isolates in patients from Southern Mexico with chronic gastritis. Another purpose of this work was to study the prevalence of virulent genotypes and distribution of resistant strains according to the vacA/cagA/babA2 H. pylori genotypes. One hundred forty-four patients with chronic gastritis were studied. Forty-five H. pylori strains were isolated and clarithromycin susceptibility was determined by the disk-diffusion method. The 82.2% of the strains had the combination of alleles vacA s1 m1 and the cagA gene was detected in 77.8% and 40% of the strains were babA2 positive. The vacA s1 m1 genotype was detected more frequently in cagA(+) strains, vacA s1m1/cagA(+)/babA2(-) genotype was more frequent than vacA s1m1/cagA(+)/babA2(+), 37.8% and 33.3%, respectively. Eight strains were clarithromycin resistant, in three of these, point mutations were identified, but only in one strain the A2143G mutation associated with clarithromycin resistance was found. Other point mutations (A1821G, G1826A, T1830C, A2089G, T1600C, C1601T, C1602T, T1610C, A1611C and T1633G) that have not been associated with clarithromycin resistance were identified. The highest proportion of resistant strains was vacA s1m1/cagA(+) (62.5%). In patients from southern Mexico with chronic gastritis, the prevalence of clarithromycin resistance is within internationally accepted range (17.8%) and allows continued use of triple therapy for H. pylori eradication. However, it is necessary to monitor the evolution of clarithromycin resistance in this area. The largest proportion of resistant H. pylori strains is not harboring the A2142G, A2142C and A2143G mutations in the 23S rRNA gene (87.5%). The vacA s1m1/cagA(+) genotype was the most prevalent and among clarithromycin-resistant strains, this was the predominant.

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“…Its MIC value (as low as 0.016-0.15 mg/L) is lower than those of other antibiotics[51]. The antibiotic binds reversibly to hairpin 35 of domain II and the peptidyl transferase loop of domain V of the 23S rRNA molecule in the 50S ribosomal subunit[1,10,33,35,37,52].…”

Section: Mechanisms Of Antimicrobial Resistancementioning

confidence: 99%

Importance of antimicrobial susceptibility testing for the management of eradication inHelicobacter pyloriinfection

Arslan

Yılmaz

Demiray-Gürbüz

2017

WJG

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The management of Helicobacter pylori (H. pylori) infection treatment differs from the common treatment protocol for other infectious diseases. Because culture- or molecular-guided approaches face several practical issues, such as the invasive procedures required to obtain gastric biopsy specimens and the lack of availability of routine laboratory testing in some places, H. pylori treatment includes the administration of two or three empirically selected antibiotics combined with a proton pump inhibitor rather than evidence-based eradication treatment. The efficacy of empirical therapy is decreasing, mostly due to increasing multiple resistance. Multiresistance to levofloxacin, clarithromycin, and metronidazole, which are commonly used in empirical treatments, appears to have increased in many countries. Mutations play a primary role in the antimicrobial resistance of H. pylori, but many different mechanisms can be involved in the development of antibiotic resistance. Determining and understanding these possible mechanisms might allow the development of new methods for the detection of H. pylori and the determination of antimicrobial resistance. A treatment based on the detection of antimicrobial resistance is usually more effective than empirical treatment. Nevertheless, such an approach before treatment is still not recommended in the Maastricht guidelines due to the difficulty associated with the routine application of available culture- or molecular-based susceptibility tests, which are usually administered in cases of treatment failure. The management of first and rescue treatments requires further research due to the steadily increase in antimicrobial resistance.

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Antimicrobial susceptibility testing for Helicobacter pylori isolates from Brazilian children and adolescents: comparing agar dilution, E-test, and disk diffusion

Cited by 49 publications

References 30 publications

Helicobacter pylori antimicrobial resistance in a pediatric population

Helicobacter pylori antimicrobial resistance in a pediatric population

Clarithromycin resistance and prevalence of Helicobacter pylori virulent genotypes in patients from Southern México with chronic gastritis

Importance of antimicrobial susceptibility testing for the management of eradication inHelicobacter pyloriinfection

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