Occurrence and molecular characterization of Cucumber green mottle mosaic virus in cucurbit crops of KPK, Pakistan

Abstract

Field survey of the cucurbit crops revealed a high incidence of Cucumber green mottle mosaic virus (CGMMV) in Khyber Pakhtunkhwa Province (KPK), Pakistan. Among the seven districts surveyed, average percent incidence of CGMMV was recorded up to 58.1% in district Nowshera, followed by 51.1% in district Charsada, 40.5% in district Swabi and 37.3% in district Mardan. In Swat and Dir districts average incidence CGMMV was recorded upto 31.2% and 29.4%, respectively. Among the different crops highest incidence in plain areas of KPK was recorded in bottle gourd (59.3%) followed by 56.3% in Squash, 54.5% in Pumpkin, 45.5% in Melon, 41.7% in Cucumber and 29.9% in Sponge gourd. In Northern hilly areas highest incidence of CGMMV (52.9%) was observed in pumpkin, followed by 49.6% in bottle gourd, 47.3% in squash, 45.1% in Melon 42.3% in cucumber and 41.6% in sponge gourd. Little variability was observed in the coat protein amino acid sequence identities of CGMMV Pakistan isolate, when compared with other reported isolates.

Transcriptome analysis of watermelon (Citrullus lanatus) fruits in response to Cucumber green mottle mosaic virus (CGMMV) infection

“…Cucumber green mottle mosaic virus (CGMMV) is a member of the Tobamovirus genus in the Virgaviridae family and is an important viral pathogen of cucurbit crops worldwide 1 – 4 . CGMMV usually produces typical mosaic patterning on the leaves of infected plants and causes fruits deformation 5 , 6 , and ultimately results in yield reduction and considerable economic losses 1 , 7 , 8 . CGMMV was first reported from the UK in 1935 9 and has subsequently rapidly spread to several countries, including Greece 10 , Japan 11 , Korea 12 , Israel 1 , Pakistan 13 , India 14 , America 15 and Canada 16 .…”

Genetic diversity of cucumber green mottle mosaic virus (CGMMV) infecting cucurbits

“…The family Cucurcbitaceae includes several edible and non-edible species that are being grown across the globe particularly in tropical regions warm temperature ( Ali et al, 2014; Asad et al, 2022 ). Economically important species of the family Cucurbitaceae are cucumber ( Cucumis sativus ), bottle gourd ( Lagenaria siceraria ) , melon ( Cucumis melo L. ), sponge gourd ( Luffa acutangula ) , summer squash ( Cucurbita pepo ) , bitter gourd ( Momordica charantia ) and serpent gourd ( Cucumis melo var.…”

First Detection and Characterization of Chrysanthemum virus B infecting Chrysanthemum in Thailand

“…Chrysanthemum leaves from different cultivars showing viruslike disease, such as mild mottling, vein clearing, and yellowing were collected for detection of CVB. The percentage of disease incidence (PDI) was calculated as described by [1].…”