Lack of AHL-based quorum sensing in Pseudomonas fluorescens isolated from milk

Martins, Maurílio Lopes; Pinto, Uelinton Manoel; Riedel, Katharina; Vanetti, Maria Cristina Dantas; Mantovani, Hilário Cuquetto; Araújo, Elza Fernandes de

doi:10.1590/s1517-83822014000300037

Cited by 29 publications

(21 citation statements)

References 43 publications

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“…As described by Martins et al . [64], quorum sensing systems based on N-acyl-homoserine lactone (AHL) signalling molecules have been identified in a few P . fluorescens strains [37,65–68].…”

Section: Resultsmentioning

confidence: 99%

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Contribution of the Pseudomonas fluorescens MFE01 Type VI Secretion System to Biofilm Formation

et al. 2017

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Type VI secretion systems (T6SSs) are widespread in Gram-negative bacteria, including Pseudomonas. These macromolecular machineries inject toxins directly into prokaryotic or eukaryotic prey cells. Hcp proteins are structural components of the extracellular part of this machinery. We recently reported that MFE01, an avirulent strain of Pseudomonas fluorescens, possesses at least two hcp genes, hcp1 and hcp2, encoding proteins playing important roles in interbacterial interactions. Indeed, P. fluorescens MFE01 can immobilise and kill diverse bacteria of various origins through the action of the Hcp1 or Hcp2 proteins of the T6SS. We show here that another Hcp protein, Hcp3, is involved in killing prey cells during co-culture on solid medium. Even after the mutation of hcp1, hcp2, or hcp3, MFE01 impaired biofilm formation by MFP05, a P. fluorescens strain isolated from human skin. These mutations did not reduce P. fluorescens MFE01 biofilm formation, but the three Hcp proteins were required for the completion of biofilm maturation. Moreover, a mutant with a disruption of one of the unique core component genes, MFE01ΔtssC, was unable to produce its own biofilm or inhibit MFP05 biofilm formation. Finally, MFE01 did not produce detectable N-acyl-homoserine lactones for quorum sensing, a phenomenon reported for many other P. fluorescens strains. Our results suggest a role for the T6SS in communication between bacterial cells, in this strain, under biofilm conditions.

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Section: Resultsmentioning

confidence: 99%

“…Several studies have shown that other strains of P . fluorescens do not produce AHLs [64,69,70]. Standalone blast analyses of the MFE01 draft genome indicated that it did not contain any genes encoding a protein corresponding to the conserved AHL synthase (WP_044464955.1).…”

Section: Resultsmentioning

confidence: 99%

Contribution of the Pseudomonas fluorescens MFE01 Type VI Secretion System to Biofilm Formation

et al. 2017

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“…Through the quorum sensing system bacteria are capable of performing tasks as a group such as migration to more favorable environments, biofilm formation, virulence gene expression, bacteriocin and antibiotic production, bioluminescence, pigment production, among others (Martins et al, 2014;Skandamis & Nychas, 2012). The system has also been shown to regulate bacterial behaviors in food ecosystems highlighting the importance of understanding its control in order to improve food safety and quality (Bai & Rai, 2011;Pinto et al, 2007;Skandamis & Nychas, 2012).…”

Section: Introductionmentioning

confidence: 99%

Anti-quorum sensing activity of phenolic extract from Eugenia brasiliensis (Brazilian cherry)

et al. 2016

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The aim of this study was to assess the anti-quorum sensing activity of phenolic extracts from grumixama (Eugenia brasiliensis), also known as Brazilian cherry, in concentrations that did not interfere with bacterial growth. The pulp phenolic compounds were extracted by using solid phage extraction in a mini-collumn C18 and quantified by spectrophotometry. The anti-quorum sensing activity was evaluated by testing the inhibition of violacein production in Chromobacterium violaceum and by evaluating the swarming motility in Aeromonas hydrophila and Serratia marcescens, both phenotypes regulated by quorum sensing. The phenolic extract strongly inhibited the production of violacein in C. violaceum, reducing its production in comparison with a control with no extract. No inhibition of growth was observed at the concentrations tested for quorum sensing inhibition. Confirming the quorum sensing inhibition phenotype, the extract was also able to inhibit swarming motility in S. marcescens and in A. hydrophila, although in the later the effect was marginal. Dverall, these results indicate that phenolic extract from E. brasiliensis presents quorum sensing inhibitory activity most likely due to the presence of fruit phenolics which have been implicated as quorum sensing inhibitors in Gram negative bacteria.Keywords: quorum sensing; anti-quorum sensing; phenolic extract; Brazilian cherry; Eugenia brasiliensis. Practical Application:The phenolic extract in this study presents potential applications in the food and pharmaceutical industries due to inhibition of phenotypes regulated by quorum sensing, which include production of virulence factors and spoilage enzymes. By inhibiting phenotypes associated with quorum sensing, the extract presents potential application as a natural preservative for food conservation and as an alternative or an adjuvant with antibiotics.

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“…() and Martins et al. () both found that two highly proteolytic P. fluorescens strains (07A and 041), which were isolated from raw milk, did not produce AHL molecules in any of the tested media. In addition, synthetic AHLs added to the medium did not influence the production of proteases (Pinto et al., ).…”

Section: Biological Properties Of Protease Aprxmentioning

confidence: 95%

“…Juhas, Eberl, and Tümmler (2005), and Liu, Wang, and Griffiths (2007) reported that quorum sensing systems are widespread among pseudomonads. However, Pinto et al (2010) and Martins et al (2014) both found that two highly proteolytic P. fluorescens strains (07A and 041), which were isolated from raw milk, did not produce AHL molecules in any of the tested media. In addition, synthetic AHLs added to the medium did not influence the production of proteases (Pinto et al, 2010).…”

Section: Regulation Of Aprx Productionmentioning

confidence: 98%

The Extracellular Protease AprX from Pseudomonas and its Spoilage Potential for UHT Milk: A Review

Zhang

Bijl

Svensson

et al. 2019

Comp Rev Food Sci Food Safe

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The negative effects of proteases produced by psychrotrophic bacteria on dairy products, especially ultra‐high‐temperature (UHT) milk, are drawing increasing attention worldwide. These proteases are especially problematic, because it is difficult to control psychrotrophic bacteria during cold storage and to inactivate their heat‐resistant proteases during dairy processing. The predominant psychrotrophic species with spoilage potential in raw milk, Pseudomonas, can produce a thermostable extracellular protease, AprX. A comprehensive understanding of AprX on the aspects of its biological properties, regulation, proteolytic potential, and its impact on UHT milk can contribute to finding effective approaches to minimize, detect, and inactivate AprX. AprX also deserves attention as a representative of all extracellular metalloproteases produced by psychrotrophic bacteria in milk. The progress of current research on AprX is summarized in this review, including a view on the gap in current understanding of this enzyme. Reducing the production and activity of AprX has considerable potential for alleviating the problems that arise from the instability of UHT milk during shelf‐life.

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Lack of AHL-based quorum sensing in Pseudomonas fluorescens isolated from milk

Cited by 29 publications

References 43 publications

Contribution of the Pseudomonas fluorescens MFE01 Type VI Secretion System to Biofilm Formation

Contribution of the Pseudomonas fluorescens MFE01 Type VI Secretion System to Biofilm Formation

Anti-quorum sensing activity of phenolic extract from Eugenia brasiliensis (Brazilian cherry)

The Extracellular Protease AprX from Pseudomonas and its Spoilage Potential for UHT Milk: A Review

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