Whole cells in enantioselective reduction of benzyl acetoacetate

Abstract

The β-ketoester benzyl acetoacetate was enantioselectively reduced to benzyl (S)-3-hydroxybutanoate by seven microorganism species. The best result using free cells was obtained with the yeast Hansenula sp., which furnished 97% ee and 85% of conversion within 24 h. After immobilization in calcium alginate spheres, K.marxianus showed to be more stable after 2 cycles of reaction.

Enantioselective Conversion of Racemic Felodipine to S(-)-Felodipine by <i>Aspergillus niger</i> and Lipase AP6 Enzyme

“…reduction of benzyl acetoacetate to benzyl (S)-3-hydroxybutanoate with high enantiomeric excess (76%) and conversion rate (99%) than the free cells which resulted in low enantiomeric excess and conversion values (68% and 80% respectively) [33]. The results were also in line with Tamalampudi et al (2007) and according to them (RS)-1phenylethanol was enantioselectively transesterified to (R)-1-phenylethyl acetate by using immobilized recombinant Aspergillus oryzae expressing lipase-encoding gene from Candida antarctica, which resulted with an enantiomeric excess (ee) of >99%.…”