Evaluation of tests to predict metallo-&amp;#946;-lactamase in cystic fibrosis (CF) and non-(CF) Pseudomonas

Perez, Leandro Reus Rodrigues; Limberger, Mariana Fagundes; Costi, Ricardo Miguel; Dias, Cícero; Barth, Afonso Luís

doi:10.1590/s1517-83822014000300011

Cited by 5 publications

(3 citation statements)

References 11 publications

(16 reference statements)

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“…Pseudomonas aeruginosa is considered as one of the most important opportunistic Gram-negative pathogen that can cause many human infections including life-threatening (Lihua et al, 2013). Antibiotic resistance against this pathogen happens naturally as well as its ability to acquire resistance to a wide range of antimicrobial classes, make infections caused by this species of bacteria difficult to manage (Perez et al, 2014). The antipseudomonal agents are very limited and categorized into three major antimicrobial classes: lactams, aminoglycosides and fluoroquinolones (Giamarellou and Antoniadou, 2001).…”

Section: Introductionmentioning

confidence: 99%

Phenotypic and molecular characterization of fluoroquinolone resistant Pseudomonas aeruginosa isolates in Palestine

Adwan

Omar

2022

Braz. J. Biol.

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Fluoroquinolones are important antimicrobial agents for the treatment of Pseudomonas infections. A total of 11 isolates of P. aeruginosa were collected from different clinical samples from different medical centers in the North West Bank-Palestine during 2017. In this study, resistance to fluoroquinolones and secretions of β-lactamases were detected by phenotypic methods, while presence of β-lactamase gene sequences and other virulence factors were detected by PCR technique. PCR product for gyrA, parC and parE genes were sequenced for further analyses. The phylogenetic analyses, population diversity indices and haplotypes determination were conducted using computer programs MEGA version 6, DnaSP 5.1001 and median-joining algorithm in the program Network 5, respectively. Results of this study showed that the MIC for ciprofloxacin and norfloxacin had a range of 32-256 µg/ml. In addition, all isolates carried either exoT or exoT and exoY genes, different β-lactamase genes and 82% of these isolates harbored class 1 integrons. Analyses of the gyrA, parC and parE sequences were found to be polymorphic, had high haplotype diversity (0.945-0.982), low nucleotide diversity (0.01225-0.02001) and number of haplotypes were 9 for each gyrA and parE genes and 10 haplotypes for parC gene. The founder haplotypes being Hap-1 (18%), Hap-2 (27.3%) and Hap-6 (9.1%) for gyrA, parC and parE genes, respectively. Two of ParE haplotypes were detected as indel haplotypes. The Median-joining- (MJ) networks constructed from haplotypes of these genes showed a star-like expansion. The neutrality tests (Tajima’s D test and Fu’s Fs test) for these genes showed negative values. Palestinian fluoroquinolone resistant P. aeruginosa strains showed high MIC level for fluoroquinolones, β-lactamase producers, carried type III secretion exotoxin-encoding genes, most of them had integrase I gene and had high level of mutations in QRDR regions in gyrA, parC and parE genes. All these factors may play an important role in the invasiveness of these strains and make them difficult to treat. Isolation of these strains from different medical centers, indicate the need for a strict application of infection control measures in Medical centers in the North West Bank-Palestine that aim to reduce expense and damage caused by P. aeruginosa infections. Molecular analyses showed that Palestinian fluoroquinolone resistant P. aeruginosa haplotypes are not genetically differentiated; however, more mutations may exist in these strains.

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Section: Introductionmentioning

confidence: 99%

Phenotypic and molecular characterization of fluoroquinolone resistant Pseudomonas aeruginosa isolates in Palestine

Adwan

Omar

2022

Braz. J. Biol.

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“…Pseudomonas aeruginosa is an important opportunistic pathogen 1 , 2 , 3 causing life-threatening infections, especially in immunocompromised patients. 4 , 5 , 6 Often these infections are difficult to treat due to the intrinsic resistance of the species 7 as well as its remarkable ability to acquire resistance to a wide range of antimicrobial agents. 8 Fluoroquinolones are the only accessible antibiotics for effective oral treatment of infections caused by this organism.…”

Section: Introductionmentioning

confidence: 99%

The role of gyrA and parC mutations in fluoroquinolones-resistant Pseudomonas aeruginosa isolates from Iran

Nouri

Rezaee

Hasani

et al. 2016

Brazilian Journal of Microbiology

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The aim of this study was to examine mutations in the quinolone-resistance-determining region (QRDR) of gyrA and parC genes in Pseudomonas aeruginosa isolates. A total of 100 clinical P. aeruginosa isolates were collected from different university-affiliated hospitals in Tabriz, Iran. Minimum inhibitory concentrations (MICs) of ciprofloxacin and levofloxacin were evaluated by agar dilution assay. DNA sequences of the QRDR of gyrA and parC were determined by the dideoxy chain termination method. Of the total 100 isolates, 64 were resistant to ciprofloxacin. No amino acid alterations were detected in gyrA or parC genes of the ciprofloxacin susceptible or ciprofloxacin intermediate isolates. Thr-83 → Ile substitution in gyrA was found in all 64 ciprofloxacin resistant isolates. Forty-four (68.75%) of them had additional substitution in parC. A correlation was found between the number of the amino acid alterations in the QRDR of gyrA and parC and the level of ciprofloxacin and levofloxacin resistance of the P. aeruginosa isolates. Ala-88 → Pro alteration in parC was generally found in high level ciprofloxacin resistant isolates, which were suggested to be responsible for fluoroquinolone resistance. These findings showed that in P. aeruginosa, gyrA was the primary target for fluoroquinolone and additional mutation in parC led to highly resistant isolates.

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“…Causing dangerous infections, especially in immunocompromised patients (3) . This infection is difficult to treat due to the great resistance it possesses (4) . In addition; it has a great ability to resist a wide range of antimicrobial agents.…”

Section: Introductionmentioning

confidence: 99%

Effect of Exotoxin a Isolated from Pseudomonas Aeruginosa Against Human Cancer Cell Lines

Muthanna Hamid Hassan

2021

Indian Journal of Forensic Medicine & Toxicology

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In This study, the presence of Pseudomonas aeruginosa was investigated from many clinical samples, and then about 54 isolates were obtained, diagnosed with all different diagnostic methods, and then the bacterial isolates produced for Exotoxin A were detected by the ELISA method and then the most productive isolate was chosen for purification by means of Precipitation with ammonium sulphate and ion-exchange column and detection of molecular weight was (M.W 65.03KD).The competent isolation tested its toxicity against two cancer cell lines HeLa and PC3 which were an inhibition ratio 69.1% and 61.6% respectively in high concentration.

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Evaluation of tests to predict metallo-β-lactamase in cystic fibrosis (CF) and non-(CF) Pseudomonas

Cited by 5 publications

References 11 publications

Phenotypic and molecular characterization of fluoroquinolone resistant Pseudomonas aeruginosa isolates in Palestine

Phenotypic and molecular characterization of fluoroquinolone resistant Pseudomonas aeruginosa isolates in Palestine

The role of gyrA and parC mutations in fluoroquinolones-resistant Pseudomonas aeruginosa isolates from Iran

Effect of Exotoxin a Isolated from Pseudomonas Aeruginosa Against Human Cancer Cell Lines

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