Effects of different osmolarities on bacterial biofilm formation

Kavamura, Vanessa Nessner; Melo, I. S. de

doi:10.1590/s1517-83822014000200034

Cited by 12 publications

(3 citation statements)

References 26 publications

(26 reference statements)

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“…; Corona‐Izquierdo and Membrillo‐Hernandez ; Zhang et al . ; Kavamura and de Melo ), either systems that better mimic the natural conditions and environments in which clinically important biofilms are formed, such as microfluidics devices (Karimi et al . ) or biofilm infection models in animals (Kadurugamuwa et al .…”

Section: Resultsmentioning

confidence: 99%

Inhibition of quorum-sensing-dependent virulence factors and biofilm formation of clinical and environmental Pseudomonas aeruginosa strains by ZnO nanoparticles

García-Lara

Saucedo-Mora

Roldán-Sánchez

et al. 2015

Lett Appl Microbiol

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Virulence inhibition by quorum quenchers in Pseudomonas aeruginosa is usually tested in laboratory strains and studies of their effects in relevant clinical and environmental strains are scarce. This study is significant as the effects of ZnO nanoparticles in QS-dependent virulence factor production were tested in six clinical strains from cystic fibrosis patients, a C-30 resistant clinical strain from urine, two PA14 gallium resistant mutants, a PA14 C-30 resistant mutant, and four environmental isolates. ZnO nanoparticles decreased elastase, pyocyanin, and biofilms for most of the strains; indicating they have broad spectrum and may be an alternative to treat Ps. aeruginosa infections.

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Section: Resultsmentioning

confidence: 99%

Inhibition of quorum-sensing-dependent virulence factors and biofilm formation of clinical and environmental Pseudomonas aeruginosa strains by ZnO nanoparticles

García-Lara

Saucedo-Mora

Roldán-Sánchez

et al. 2015

Lett Appl Microbiol

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“…Numerous researchers have differentiated AL and SAL biofilm genetically, enzymatically, and based on cultural conditions ( Friedman and Kolter, 2004 ; Yap et al, 2005 ; Haque et al, 2012 ). Bacterial biofilm formation in different bacterial strains was shown to be influenced by the pH ( Hostacká et al, 2010 ; Ramli et al, 2012 ; Nguyen et al, 2014 ; Zhou et al, 2014 ; Haque et al, 2017 ), high osmolarity ( Lebeer et al, 2007 ; Hou et al, 2014 ; Kavamura and de Melo, 2014 ; Haque et al, 2017 ), and oxygen tension ( Gerstel and Römling, 2001 ; Bjergbæk et al, 2006 ; Ahn and Burne, 2007 ; Liang et al, 2010 ; Wu et al, 2013 ). Except temperature ( Mosharaf et al, 2018 ), effect of pH, osmolarity, and oxygen tension on biofilm formation by E. asburiae ENSD102, E. ludwigii ENSH201, Vitreoscilla sp.…”

Section: Discussionmentioning

confidence: 99%

Biofilm Formation, Production of Matrix Compounds and Biosorption of Copper, Nickel and Lead by Different Bacterial Strains

et al. 2021

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Bacterial biofilms play a key role in metal biosorption from wastewater. Recently, Enterobacter asburiae ENSD102, Enterobacter ludwigii ENSH201, Vitreoscilla sp. ENSG301, Acinetobacter lwoffii ENSG302, and Bacillus thuringiensis ENSW401 were shown to form air–liquid (AL) and solid–air–liquid (SAL) biofilms in a static condition at 28 and 37°C, respectively. However, how environmental and nutritional conditions affect biofilm formation; production of curli and cellulose; and biosorption of copper (Cu), nickel (Ni), and lead (Pb) by these bacteria have not been studied yet. In this study, E. asburiae ENSD102, E. ludwigii ENSH201, and B. thuringiensis ENSW401 developed the SAL biofilms at pH 8, while E. asburiae ENSD102 and Vitreoscilla sp. ENSG301 constructed the SAL biofilms at pH 4. However, all these strains produced AL biofilms at pH 7. In high osmolarity and ½-strength media, all these bacteria built fragile AL biofilms, while none of these strains generated the biofilms in anaerobic conditions. Congo red binding results showed that both environmental cues and bacterial strains played a vital role in curli and cellulose production. Calcofluor binding and spectrophotometric results revealed that all these bacterial strains produced significantly lesser amounts of cellulose at 37°C, pH 8, and in high osmotic conditions as compared to the regular media, at 28°C, and pH 7. Metal biosorption was drastically reduced in these bacteria at 37°C than at 28°C. Only Vitreoscilla sp. ENSG301 and B. thuringiensis ENSW401 completely removed (100%) Cu and Ni at an initial concentration of 12.5 mg l–1, while all these bacteria totally removed (100%) Pb at concentrations of 12.5 and 25 mg l–1 at pH 7 and 28°C. At an initial concentration of 100 mg l–1, the removal of Cu (92.5 to 97.8%) and Pb (89.3 to 98.3%) was the highest at pH 6, while it was higher (84.7 to 93.9%) for Ni at pH 7. Fourier transform infrared spectroscopy results showed metal-unloaded biomass biofilms contained amino, hydroxyl, carboxyl, carbonyl, and phosphate groups. The peak positions of these groups were shifted responding to Cu, Ni, and Pb, suggesting biosorption of metals. Thus, these bacterial strains could be utilized to remove Cu, Ni, and Pb from aquatic environment.

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“…Numerous researchers have differentiated AL and SAL biofilm genetically, enzymatically, and based on cultural conditions (Friedman and Kolter, 2004;Yap et al, 2005;Haque et al, 2012). Bacterial biofilm formation in different bacterial strains was shown to be influenced by the pH (Hostacká et al, 2010;Ramli et al, 2012;Nguyen et al, 2014;Zhou et al, 2014;Haque et al, 2017), high osmolarity (Lebeer et al, 2007;Hou et al, 2014;Kavamura and de Melo, 2014;Haque et al, 2017), and oxygen tension (Gerstel and Römling, 2001;Bjergbaek et al, 2006;Ahn and Burne, 2007;Liang et al, 2010;Wu et al, 2013). Except temperature (Mosharaf et al, 2018), effect of pH, osmolarity, and oxygen tension on biofilm formation by E. asburiae ENSD102, E. ludwigii ENSH201, Vitreoscilla sp.…”

Section: Environmental Conditions Affect Bacterial Biofilm Formationmentioning

confidence: 99%

Microbial Ecotoxicology Advances to Improve Environmental and Human Health Under Global Change

Cébron

Karpouzas

Martin-Laurent

et al. 2022

Frontiers Research Topics

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Frontiers is more than just an open-access publisher of scholarly articles: it is a pioneering approach to the world of academia, radically improving the way scholarly research is managed. The grand vision of Frontiers is a world where all people have an equal opportunity to seek, share and generate knowledge. Frontiers provides immediate and permanent online open access to all its publications, but this alone is not enough to realize our grand goals. Frontiers Journal SeriesThe Frontiers Journal Series is a multi-tier and interdisciplinary set of open-access, online journals, promising a paradigm shift from the current review, selection and dissemination processes in academic publishing. All Frontiers journals are driven by researchers for researchers; therefore, they constitute a service to the scholarly community. At the same time, the Frontiers Journal Series operates on a revolutionary invention, the tiered publishing system, initially addressing specific communities of scholars, and gradually climbing up to broader public understanding, thus serving the interests of the lay society, too. Dedication to QualityEach Frontiers article is a landmark of the highest quality, thanks to genuinely collaborative interactions between authors and review editors, who include some of the world's best academicians. Research must be certified by peers before entering a stream of knowledge that may eventually reach the public -and shape society; therefore, Frontiers only applies the most rigorous and unbiased reviews. Frontiers revolutionizes research publishing by freely delivering the most outstanding research, evaluated with no bias from both the academic and social point of view. By applying the most advanced information technologies, Frontiers is catapulting scholarly publishing into a new generation.

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Effects of different osmolarities on bacterial biofilm formation

Cited by 12 publications

References 26 publications

Inhibition of quorum-sensing-dependent virulence factors and biofilm formation of clinical and environmental Pseudomonas aeruginosa strains by ZnO nanoparticles

Inhibition of quorum-sensing-dependent virulence factors and biofilm formation of clinical and environmental Pseudomonas aeruginosa strains by ZnO nanoparticles

Biofilm Formation, Production of Matrix Compounds and Biosorption of Copper, Nickel and Lead by Different Bacterial Strains

Microbial Ecotoxicology Advances to Improve Environmental and Human Health Under Global Change

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