Phylogenetic grouping and pathotypic comparison of urine and fecal Escherichia coli isolates from children with urinary tract infection

Abstract

The aim of this study was to investigate the phylogenetic background and to assess hlyD (involved in the secretion of haemolysin A) and intI1 (encoding a class 1 integrase) in Escherichia coli isolates derived from urinary and fecal specimens. A total of 200 E. coli isolates was collected from patients presenting with urinary tract infection (UTI) during September 2009 to September 2010 and screened for hlyD and intI1 genes by polymerase chain reaction (PCR). Phylogenetic analysis showed that E. coli is composed of four main phylogenetic groups (A, B1, B2 and D) and that uropathogenic E. coli (UPEC) isolates mainly belong to groups B2 (54%) and D (34%) whereas group A (44%) and D (26%) are predominant among commensal E. coli isolates. In this study, hlyD was present in 26% of UPEC and 2% of commensal E. coli isolates. However, hemolytic activity was detected for 42% of UPEC and 6% of commensal E. coli isolates (p < 0.05). intI1 gene was more frequently expressed in UPEC (24%) in comparison with commensal E. coli isolates (12%). Resistance to aztreonam, co-trimoxazole and cefpodoxime were frequently found among UPEC isolates whereas commensal E. coli isolates were commonly resistant to co-trimoxazole, nalidixic acid and cefotaxime. Concluding, a considerable difference between UPEC and commensal E. coli isolates was observed regarding their phylogenetic groups, presence of class 1 integron and hlyD gene, hemolysin activity and resistance pattern. The detection of class 1 integrons and hlyD gene was higher among UPEC compared with commensal E. coli isolates. These findings may contribute for a better understanding of the factors involved in the pathogenesis of UPEC.

Resistance to antimicrobial drugs in different surface waters and wastewaters of Guadeloupe

“…The predominance of groups A (50%) and D (24%) was similar to that of E . coli of faecal origin, whereas in UTIs, the phylogenetic groups B2 and D were largely predominant [46]. Most E .…”

Identification of Extended-Spectrum β-Lactamase Genes and AmpC-β-Lactamase in Clinical Isolates of Escherichia coli Recovered from Patients with Urinary Tract Infections in Kerman, Iran

“…This finding is similar to other studies conducted in Iran. All these studies indicate that in the near future, several antibiotics such as CIP and SXT will be useless in empirical therapy of uncomplicated UTIs in the community (24,25,31). The spread of antibiotic resistance is related to healthcare-related behaviors and the environmental policies/laws in place.…”

Intestinal colonization with phylogenetic group B2 Escherichia coli related to inflammatory bowel disease: a systematic review and meta-analysis

“…In 2007, a link between E. coli of the phylogenetic groups B2 and D, and IBD was described [15]. Interestingly, UPEC isolates will often be found within the phylogenetic group B2 E. coli, whereas commensal fecal E. coli will not [25]. Since then, several studies have looked into the differences in E. coli phylogenetic groups between IBD patients and controls with a non-inflamed intestinal mucosa but all of these studies have been small, with varying results, and conclusions should therefore be made with some caution [15,[19][20][21][22][23][24].…”