Prevalence of Pseudomonas aeruginosa and Acinetobacter spp. in subgingival biofilm and saliva of subjects with chronic periodontal infection

Souto, Renata; Silva-Boghossian, Carina Maciel; Colombo, Ana Paula Vieira

doi:10.1590/s1517-83822014000200017

Cited by 82 publications

(59 citation statements)

References 43 publications

(83 reference statements)

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“…[49] reported high counts of E. faecalis, P. aeruginosa and S. aureus within buccal/gingival crevice cells from periodontitis patients. In agreement with previous investigations [26,[43][44][45][46]50] and/or sites loosing attachment or not responding successfully to periodontal treatment [18,27,51].…”

Section: Discussionsupporting

confidence: 92%

“…Regarding systemically healthy individuals presenting periodontal diseases, our current findings showed that approximately 42% of the tested species (C. albicans, enterobacteria, H. alvei, Neisseria spp., O. uli and S. marcescens) were detected in significantly higher prevalence and counts in diseased than periodontally healthy patients. Data reported by other studies have also indicated high frequencies (30-80%) of these opportunistic pathogens in the subgingival biofilm associated to chronic and/or generalized aggressive periodontitis [26,27,[43][44][45][46][47][48]. It is interesting to note that healthy sites from periodontitis patients harbored higher counts of these pathogens than sites from the H group [43,47], suggesting a more efficient intraoral dissemination of pathogens from periodontal pockets to shallow healthy sites in individuals with periodontal diseases.…”

Section: Discussionmentioning

confidence: 62%

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Periodontal-disease-associated biofilm: A reservoir for pathogens of medical importance

Colombo

Magalhães

Hartenbach

et al. 2016

Microbial Pathogenesis

233

154

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The ecological diversity of the periodontal microenvironment may provide suitable conditions for the colonization of species not usually considered members of the oral microbiota. In this investigation, we aimed to determine the prevalence and levels of pathogenic species of medical relevance in the microbiota of individuals with distinct periodontal clinical status. Subgingival biofilm was obtained from patients with periodontal health (H, n = 81), gingivitis (G, n = 55), generalized aggressive (AgP, n = 36) or chronic periodontitis (CP, n = 98), and analyzed for 39 microbial taxa using a checkerboard DNA-DNA hybridization technique. Microbial differences among groups, as well as associations between clinical and microbiological parameters were sought by non-parametric and univariate correlation tests. Neisseria spp., Peptostreptococus anaerobius, Candida albicans, enterobacteria, Pseudomonas aeruginosa, Eubacterium saphenum, Clostridium difficile and Olsenella uli were detected in high mean prevalence and counts in the subgingival microbiota of the study population. Species that were more related to periodontal inflammation and tissue destruction at the patient and site levels included enterobacteria, C. albicans, Neisseria spp., P. aeruginosa, O. uli, Hafnia alvei, Serratia marcescens and Filifactor alocis (p < 0.05). In contrast, Fusobacterium necrophorum, Lactobacillus acidophilus, Staphylococcus aureus and Streptococcus pneumoniae were associated with periodontal health (p < 0.05). Pathogenic species of medical importance may be detected in high prevalence and levels in the periodontal microbiota. Regardless of their role in periodontal health or disease, the periodontal biofilm may be a source for dissemination and development of systemic infections by these pathogenic microorganisms.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 62%

Periodontal-disease-associated biofilm: A reservoir for pathogens of medical importance

Colombo

Magalhães

Hartenbach

et al. 2016

Microbial Pathogenesis

233

154

View full text Add to dashboard Cite

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“…Among these bacteria, Ruminococcaceae and Lactobacillus have been reported to be commensal bacterial in the periodontium or gastrointestinal tract (Szkaradkiewicz et al 2011;Biddle et al 2013). Bacillus, Staphylococcus, Prevotella, Pseudomonas, Acinetobacter, Fusobacterium, and Porphyromonas have been reported to have pathogenic potential in the periodontium and gut (Szkaradkiewicz et al 2011;Hajishengallis 2014;Rolny et al 2014;Souto et al 2014). The proportion of the bacterial community composed of Ruminococcaceae and Lactobacillus in aged mice was reduced in comparison to young mice, while the proportion of bacteria with pathogenic characteristics such as Staphylococcus increased in aged mice.…”

Section: Aging Increases Colonization Of P Gingivalis and Reduces Bamentioning

confidence: 99%

Effect of Aging on Periodontal Inflammation, Microbial Colonization, and Disease Susceptibility

Dong

Xiao

et al. 2016

J Dent Res

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Periodontitis is a chronic inflammatory disease induced by a biofilm that forms on the tooth surface. Increased periodontal disease is associated with aging. We investigated the effect of aging on challenge by oral pathogens, examining the host response, colonization, and osteoclast numbers in aged versus young mice. We also compared the results with mice with lineage-specific deletion of the transcription factor FOXO1, which reduces dendritic cell (DC) function. Periodontitis was induced by oral inoculation of Porphyromonas gingivalis and Fusobacterium nucleatum in young (4 to 5 mo) and aged (14 to 15 mo) mice. Aged mice as well as mice with reduced DC function had decreased numbers of DCs in lymph nodes, indicative of a diminished host response. In vitro studies suggest that reduced DC numbers in lymph nodes of aged mice may involve the effect of advanced glycation end products on DC migration. Surprisingly, aged mice but not mice with genetically altered DC function had greater production of antibody to P. gingivalis, greater IL-12 expression, and more plasma cells in lymph nodes following oral inoculation as compared with young mice. The greater adaptive immune response in aged versus young mice was linked to enhanced levels of P. gingivalis and reduced bacterial diversity. Thus, reduced bacterial diversity in aged mice may contribute to increased P. gingivalis colonization following inoculation and increased periodontal disease susceptibility, reflected by higher TNF levels and osteoclast numbers in the periodontium of aged versus young mice.

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“…Also, the roughness of the colonization surface was important for biofilm colonization other than the physio-chemical properties [53,54]. Recent studies have shown that A. actinomycetemcomitans, A. baumanni and the “red complex” ( P. gingivalis, T. forsythia, T. denticola ) associated with P. aeruginosa in the subgingival microbiota increased the likelihood of periodontitis [55]. Also P. aeruginosa showed synergism with A. actinomycetemcomitans in increasing the risk of periodontal disease.…”

Section: Supragingival Biofilmsmentioning

confidence: 99%

The Biofilm Community: Rebels with a Cause

Aruni

Dou

Mishra

et al. 2015

Curr Oral Health Rep

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Oral Biofilms are one of the most complex and diverse ecosystem developed by successive colonization of more than 600 bacterial taxa. Development starts with the attachment of early colonizers such as Actinomyces species and oral streptococci on the acquired pellicle and tooth enamel. These bacteria not only adhere to tooth surface but also interact with each other and lay foundation for attachment of bridging colonizer such as Fusobacterium nucleatum followed by late colonizers including the red complex species: Porphyromonas gingivalis, Tannerella forsythia and Treponema denticola-the founders of periodontal disease. As the biofilm progresses from supragingival sites to subgingival sites, the environment changes from aerobic to anaerobic thus favoring the growth of mainly Gram-negative obligate anaerobes while restricting the growth of the early Gram-positive facultative aerobes. Microbes present at supragingival level are mainly related to gingivitis and root-caries whereas subgingival species advance the destruction of teeth supporting tissues and thus causing periodontitis. This review summarizes our present understanding and recent developments on the characteristic features of supra- and subgingival biofilms, interaction between different genera and species of bacteria constituting these biofilms and draws our attention to the role of some of the recently discovered members of the oral community.

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Prevalence of Pseudomonas aeruginosa and Acinetobacter spp. in subgingival biofilm and saliva of subjects with chronic periodontal infection

Cited by 82 publications

References 43 publications

Periodontal-disease-associated biofilm: A reservoir for pathogens of medical importance

Periodontal-disease-associated biofilm: A reservoir for pathogens of medical importance

Effect of Aging on Periodontal Inflammation, Microbial Colonization, and Disease Susceptibility

The Biofilm Community: Rebels with a Cause

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