Ethanol production from agricultural wastes using Sacchromyces cervisae

Irfan, Muhammad; Nadeem, Muhammad; Syed, Quratualain

doi:10.1590/s1517-83822014000200012

Cited by 46 publications

(18 citation statements)

References 32 publications

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“…Cellulose content in KG was measured by method described by Krishna and Ranjhan [21], lignin and delignification was measured as described by Irfan et al [22]. The saccharified biomass was passed through four layers of muslin cloth and washed with water to remove the microbial biomass and then dried overnight at 1058C to estimate the dry weight of the substrate.…”

Section: Methodsmentioning

confidence: 99%

Kallar grass (Leptochloa fusca L. Kunth) as a feedstock for ethanol fermentation with the aid of response surface methodology

Gul

Irfan

Nadeem

et al. 2017

Env Prog and Sustain Energy

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In this study, Leptochloa fusca L. Kunth or Kallar grass (KG) was thermochemically (0.625M NaOH solution followed by steam treatment at 121°C for 1 h) pretreated and utilized as a substrate for ethanol production in simultaneous saccharification and fermentation process. A four‐factor, full factorial, rotatable central composite design of response surface methodology was used to develop a statistical model for the optimization of process variables such as substrate concentration, enzyme concentration, temperature, and time. Analysis of variance for ethanol production and the substrate conversion efficiency (SCE) by Kluyveromyces marxianus demonstrated that 10% pretreated KG, 0.6 mg/mL enzyme concentration, 40°C temperature and 48 h of simultaneous saccharification and fermentation time were the optimized variables. At optimum factor setting, predicted values of ethanol production and SCE were 30 g/L and 82%, respectively. The Experimental/validation experiment was in close agreement with the predictive model and the results obtained was 40 g/L and 83.74% for ethanol production and SCE, respectively. © 2017 American Institute of Chemical Engineers Environ Prog, 37: 569–576, 2018

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Section: Methodsmentioning

confidence: 99%

Kallar grass (Leptochloa fusca L. Kunth) as a feedstock for ethanol fermentation with the aid of response surface methodology

Gul

Irfan

Nadeem

et al. 2017

Env Prog and Sustain Energy

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“…Hemicellulose was estimated by method as described by Van soest and coworker . Delignification was calculated using following formula .

Delignification true(% true) = \frac{L_{normalu} - L_{normalt}}{L_{normalu}} \times 100

where L u = Lignin contents/g of untreated dry biomass and L t = Lignin contents/g of treated dry biomass.…”

Section: Methodsmentioning

confidence: 99%

“…Hemicellulose was estimated by method as described by Van soest and coworker [14]. Delignification was calculated using following formula [15].…”

Section: Methodsmentioning

confidence: 99%

“…After optimizing the results of chemical pretreatment, further study was conducted by applying simultaneous thermophysiochemical treatment on S. spontaneum (Sarkanda grass) to expose maximum cellulose and delignification. During thermo-physiochemical pretreatment, the substrate was soaked for 2 h and then autoclaved at 1218C for different intervals of time (15,30,45, and 60 min) at optimized (2.5%) concentration of NaOH. According to a pervious study, maximum cellulose (83%) and delignification (81%) was achieved when the substrate wheat straw was treated with 2.5% NaOH at 1218C for 90 min [20].…”

Section: Thermo-physical Pretreatmentmentioning

confidence: 99%

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Effect of NaOH on delignification of Saccharum spontaneum

Ashgar

Nadeem

Irfan

et al. 2015

Env Prog and Sustain Energy

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Saccharum spontaneum abundantly available in Pakistan has great potential for production of bioethanol due to high cellulosic contents. In the present study, S. spontaneum grass was impregnated in various concentrations of NaOH (0.5%–3% wt/vol) for different soaking periods (1 h, 2 h, 4 h, 6 h, 12 h, 24 h, and 48 h). The results showed that relative cellulose contents and delignification increased with the increase in concentration of alkali and soaking time. However, maximum cellulose content and delignification (52.5% and 47.6%) was observed with 2.5% wt/vol NaOH after 24 h of soaking period. In physiochemical pretreatment, the substrate was soaked in alkaline solution for 2 h followed by steaming at 121°C for various intervals (15–60 min). Maximum relative cellulose content (81.2%) was achieved after 60 min while delignification was found 74%. These findings indicated that proper concentration of alkali as well as steaming period at specific temperature play a major role in exposure of maximum cellulosic contents of S. spontaneum to exploit it as a potential substrate for bio‐fuel production. © 2015 American Institute of Chemical Engineers Environ Prog, 35: 284–288, 2016

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“…Moreover, the peak at 1246 cm -1 (Wang et al, 2004), the indicator of aromatic C=O stretching out of lignin, decreased in intensity in the combination pretreatment RS. The carbohydrate-related peaks at 1161 cm -1 was assigned to the C-O-C vibration in cellulose and hemicellulose(Irfan et al, 2014), which also decreased in intensity after combination pretreatment, These results indicated that the lignin, cellulose and/or hemicellulose in RS were modified and even degraded by combination pretreatment.…”

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confidence: 78%