Metabolite profiling of Schizochytrium sp. by GC-MS, an oleaginous microbial source of biodiesel

Mioso, Roberto; Marante, Francisco Javier Toledo; Gonzalez, J. E.; Rodríguez, Jorge Pérez; Laguna, Irma Herrera Bravo de

doi:10.1590/s1517-83822014000200006

Cited by 10 publications

(4 citation statements)

References 24 publications

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“…Overall, these features make Schizochytrium sp . an attractive species to be used for food purposes and as a valuable source of immunostimulatory compounds for animals and humans (Mioso et al, 2014 ). The relationship between Schizochytrium sp .…”

Section: Relevant Characteristics Of Schizochytrium Sp mentioning

confidence: 99%

Prospects on the Use of Schizochytrium sp. to Develop Oral Vaccines

Ramos‐Vega¹,

Rosales‐Mendoza

Bañuelos-Hernández

et al. 2018

Front. Microbiol.

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Although oral subunit vaccines are highly relevant in the fight against widespread diseases, their high cost, safety and proper immunogenicity are attributes that have yet to be addressed in many cases and thus these limitations should be considered in the development of new oral vaccines. Prominent examples of new platforms proposed to address these limitations are plant cells and microalgae. Schizochytrium sp. constitutes an attractive expression host for vaccine production because of its high biosynthetic capacity, fast growth in low cost culture media, and the availability of processes for industrial scale production. In addition, whole Schizochytrium sp. cells may serve as delivery vectors; especially for oral vaccines since Schizochytrium sp. is safe for oral consumption, produces immunomodulatory compounds, and may provide bioencapsulation to the antigen, thus increasing its bioavailability. Remarkably, Schizochytrium sp. was recently used for the production of a highly immunoprotective influenza vaccine. Moreover, an efficient method for transient expression of antigens based on viral vectors and Schizochytrium sp. as host has been recently developed. In this review, the potential of Schizochytrium sp. in vaccinology is placed in perspective, with emphasis on its use as an attractive oral vaccination vehicle.

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Section: Relevant Characteristics Of Schizochytrium Sp mentioning

confidence: 99%

Prospects on the Use of Schizochytrium sp. to Develop Oral Vaccines

Ramos‐Vega¹,

Rosales‐Mendoza

Bañuelos-Hernández

et al. 2018

Front. Microbiol.

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“…under different oxygen conditions . This metabolomics method was also used to analyze the metabolomics profile of the final fermentative cells of Schizochytrium sp …”

Section: Introductionmentioning

confidence: 99%

Metabolomics analysis reveals 6‐benzylaminopurine as a stimulator for improving lipid and DHA accumulation of Aurantiochytriumsp.

Sun

Zheng

et al. 2016

J of Chemical Tech & Biotech

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BACKGROUNDPhytohormones are chemical messengers that have a positive effect on biodiesel production of microalgae at low concentrations. However, the effect of phytohormone 6‐benzylaminopurine on lipid and docosahexaenoic acid (DHA) production in marine DHA‐producer Aurantiochytrium has never been reported. In this study, a GC‐MS‐based metabolomics method combined with a multivariate analysis is applied to reveal the metabolic mechanism of 6‐benzylaminopurine enhancing production of lipid and DHA in Aurantiochytrium sp.YLH70.RESULTSIn total, 71 metabolites were identified by GC‐MS. The PCA model revealed that 76.9% of metabolite variation was related to 6‐benzylaminopurine treatment, and overall metabolomics profiles between the 6‐benzylaminopurine and control groups were clearly discriminated. Forty‐six metabolites identified by the PLS‐DA model were responsible for responding to 6‐benzylaminopurine. Metabolic analysis showed that 6‐benzylaminopurine could accelerate the rate of utilization of glucose in Aurantiochytrium sp. YLH70, and the metabolic flux from glycolysis, TCA cycle and mevalonate pathway to fatty acids biosynthesis was promoted. Moreover, the anti‐stress mechanism in Aurantiochytrium sp.YLH70 might be induced by 6‐benzylaminopurine.CONCLUSIONMetabolomics is a suitable tool to discover the metabolic mechanism for improving lipid and DHA accumulation in a microorganism. 6‐benzylaminopurine has the potential to stimulate lipid and DHA production of Aurantiochytrium sp.YLH70 for industrial purposes. © 2015 The Authors. Journal of Chemical Technology & Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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“…A GC-MS chemical screening on the biomass of a marine protist of the Schizochytrium genus enables the authors [43] to identify 24 kinds of organic compounds belonging to the n-alkanes, 1-alkenes, 1-alkanols, free fatty acids, methyl and ethyl esters of saturated and unsaturated fatty acids, saturated and unsaturated glycerides, wax esters, sterols, mono-, sesqui-and triterpenes. Thus, this organism from the base of the food chain, which accumulates so many nutrients and does not produce toxins, has been proposed as a very interesting specimen for modern functional nutrition.…”

Section: Resultsmentioning

confidence: 99%

Marine Environmental Metabolomics

Marante¹

2016

Metabolomics - Fundamentals and Applications

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Marine environmental metabolomics studies the interactions of marine organisms with their environment using metabolomics to characterise these interactions. There are many advantages in using this method to study interactions between organisms and the environment and to assess the function and health of organisms at the molecular level. In fact, metabolomics is finding an increasing number of applications in the marine sciences. These range from understanding the response of organisms to abiotic pressure to researching the response of organisms to other biota. These interactions can be studied at different levels, from individuals to populations for more traditional ecophysiological or ecological studies. Marine organisms have developed a high diversity of chemical defences to avoid predators and parasites. This study therefore highlights the complexity of chemical interactions in the marine environment. The research methods include 1 Hand 13 C-NMR spectroscopy, mass spectrometry, analytical and preparative chromatography, and a multitude of bio-assays.

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Metabolite profiling of Schizochytrium sp. by GC-MS, an oleaginous microbial source of biodiesel

Cited by 10 publications

References 24 publications

Prospects on the Use of Schizochytrium sp. to Develop Oral Vaccines

Prospects on the Use of Schizochytrium sp. to Develop Oral Vaccines

Metabolomics analysis reveals 6‐benzylaminopurine as a stimulator for improving lipid and DHA accumulation of Aurantiochytriumsp.

Marine Environmental Metabolomics

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