Volatile fatty acids influence on the structure of microbial communities producing PHAs

Ciesielski, Sławomir; Przybyłek, Grzegorz

doi:10.1590/s1517-83822014000200005

Cited by 11 publications

(7 citation statements)

References 22 publications

(23 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Genetic analysis revealed that Thauera sp. predominated in the reactor with acetate, while Paracoccus denitrificans predominated in the reactor with propionate [86]. Additionally, Burniol-Figols et al [82], using fermented crude glycerol and mixed microbial consortia, confirmed the possibility of selectively converting VFAs into PHAs.…”

Section: Synthesis Of Phas From Vfas By Mixed Microbial Culturesmentioning

confidence: 87%

Volatile Fatty Acids as Carbon Sources for Polyhydroxyalkanoates Production

et al. 2021

Self Cite

View full text Add to dashboard Cite

Waste of industrial origin produced from synthetic materials are a serious threat to the natural environment. The ending resources of fossil raw materials and increasingly restrictive legal standards for the management of plastic waste have led to research on the use of biopolymers, which, due to their properties, may be an ecological alternative to currently used petrochemical polymers. Polyhydroxyalkanoates (PHAs) have gained much attention in recent years as the next generation of environmentally friendly materials. Currently, a lot of research is being done to reduce the costs of the biological process of PHA synthesis, which is the main factor limiting the production of PHAs on the industrial scale. The volatile fatty acids (VFAs) produced by anaerobic digestion from organic industrial and food waste, and various types of wastewater could be suitable carbon sources for PHA production. Thus, reusing the organic waste, while reducing the future fossil fuel, originated from plastic waste. PHA production from VFAs seem to be a good approach since VFAs composition determines the constituents of PHAs polymer and is of great influence on its properties. In order to reduce the overall costs of PHA production to a more reasonable level, it will be necessary to design a bioprocess that maximizes VFAs production, which will be beneficial for the PHA synthesis. Additionally, a very important factor that affects the profitable production of PHAs from VFAs is the selection of a microbial producer that will effectively synthesize the desired bioproduct. PHA production from VFAs has gained significant interest since VFAs composition determines the constituents of PHA polymer. Thus far, the conversion of VFAs into PHAs using pure bacterial cultures has received little attention, and the majority of studies have used mixed microbial communities for this purpose. This review discusses the current state of knowledge on PHAs synthesized by microorganisms cultured on VFAs.

show abstract

Section: Synthesis Of Phas From Vfas By Mixed Microbial Culturesmentioning

confidence: 87%

Volatile Fatty Acids as Carbon Sources for Polyhydroxyalkanoates Production

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Thus, in the last case (5.0 V and 7.0 V tests), the maximum PHA yield reached was 14.2 and 8.5% despite the butyric acid concentration was similar or even lower than in 1.5 B. PHA titer values are highly dependent on the operational conditions and configuration of the process for PHA production from VFAs by MMC. Ciesielski and Przybylek [51] observed PHA titers between 227.8 (acetic as feeding) and 673 mg/L (propionic as feeding) in batch reactors inoculated with activated sludge from wastewater treatment plant. In the present study, propionic was always under 70 mg/L in feeding while acetic acid was fed between 704 and 2426 mg/L and the PHA titer was over 200 mg/L just in 1.5B and 2.5B assays where acetic was in lower levels but higher butyric concentrations where supplied.…”

Section: Pha Yieldmentioning

confidence: 99%

Influence of the total concentration and the profile of volatile fatty acids on polyhydroxyalkanoates (PHA) production by mixed microbial cultures

Bravo-Porras

Fernández-Güelfo

Álvarez-Gallego

et al. 2021

Biomass Conv. Bioref.

View full text Add to dashboard Cite

Polyhydroxyalkanoates (PHAs) production from lignocellulosic biomass using mixed microbial cultures (MMC) is a potential cheap alternative for reducing the use of petroleum-based plastics. In this study, an MMC adapted to acidogenic effluent from dark fermentation (DF) of exhausted sugar beet cossettes (ESBC) has been tested in order to determine its capability to produce PHAs from nine different synthetic mixtures of volatile fatty acids (VFAs). The tests consisted of mixtures of acetic, propionic, butyric, and valeric acids in the range of 1.5–9.0 g/L of total acidity and with three different valeric:butyric ratios (10:1, 1:1, and 1:10). Experimental results have shown a consistent preference of the MMC for the butyric and valeric acids as carbon source instead other shorter acids (propionic or acetic) in terms of PHA production yield (estimated in dry cell weight basis), with a maximum value of 23% w/w. Additionally, valeric-rich mixtures have demonstrated to carry out a fast degradation process but with poor final PHA production compared with high butyric mixtures. Finally, high initial butyric and valeric concentrations (1.1 g/L and 4.1 g/L) have demonstrated to be counterproductive to PHA production.

show abstract

“…The end product spectrum of VFA is another crucial factor in post stream and upstream applications. For example, acetic acid dominant VFA as a carbon source achieved the highest polyhydroxyalkanoates (PHA) production yield (Ciesielski and Przybylek, 2014;Kedia et al, 2014) and acetic acid has been used as an efficient carbon source for denitrification processes (Du et al, 2019). Furthermore, every industry has different feedstock requirements, for example, acetic acid has been extensively used in the polymer industry for the production of vinyl acetate monomer (Gunjan and Haresh, 2020).…”

Section: Introductionmentioning

confidence: 99%

Bioaugmented Mixed Culture by Clostridium aceticum to Manipulate Volatile Fatty Acids Composition From the Fermentation of Cheese Production Wastewater

Atasoy

Çetecioğlu

2021

Front. Microbiol.

View full text Add to dashboard Cite

Production of targeted volatile fatty acid (VFA) composition by fermentation is a promising approach for upstream and post-stream VFA applications. In the current study, the bioaugmented mixed microbial culture by Clostridium aceticum was used to produce an acetic acid dominant VFA mixture. For this purpose, anaerobic sequencing batch reactors (bioaugmented and control) were operated under pH 10 and fed by cheese processing wastewater. The efficiency and stability of the bioaugmentation strategy were monitored using the production and composition of VFA, the quantity of C. aceticum (by qPCR), and bacterial community profile (16S rRNA Illumina Sequencing). The bioaugmented mixed culture significantly increased acetic acid concentration in the VFA mixture (from 1170 ± 18 to 122 ± 9 mgCOD/L) compared to the control reactor. Furthermore, the total VFA production (from 1254 ± 11 to 5493 ± 36 mgCOD/L) was also enhanced. Nevertheless, the bioaugmentation could not shift the propionic acid dominancy in the VFA mixture. The most significant effect of bioaugmentation on the bacterial community profile was seen in the relative abundance of the Thermoanaerobacterales Family III. Incertae sedis, its relative abundance increased simultaneously with the gene copy number of C. aceticum during bioaugmentation. These results suggest that there might be a syntropy between species of Thermoanaerobacterales Family III. Incertae sedis and C. aceticum. The cycle analysis showed that 6 h (instead of 24 h) was adequate retention time to achieve the same acetic acid and total VFA production efficiency. Biobased acetic acid production is widely applicable and economically competitive with petroleum-based production, and this study has the potential to enable a new approach as produced acetic acid dominant VFA can replace external carbon sources for different processes (such as denitrification) in WWTPs. In this way, the higher treatment efficiency for WWTPs can be obtained by recovered substrate from the waste streams that promote a circular economy approach.

show abstract

Volatile fatty acids influence on the structure of microbial communities producing PHAs

Cited by 11 publications

References 22 publications

Volatile Fatty Acids as Carbon Sources for Polyhydroxyalkanoates Production

Volatile Fatty Acids as Carbon Sources for Polyhydroxyalkanoates Production

Influence of the total concentration and the profile of volatile fatty acids on polyhydroxyalkanoates (PHA) production by mixed microbial cultures

Bioaugmented Mixed Culture by Clostridium aceticum to Manipulate Volatile Fatty Acids Composition From the Fermentation of Cheese Production Wastewater

Contact Info

Product

Resources

About