Prevalence and phenotypic characterization of Enterococcus spp. isolated from food in Brazil

Camargo, Carlos Henrique; Bruder‐Nascimento, Ariane; Lee, Sarah Hwa In; Júnior, Ary Fernandes; Kaneno, Ramon; Rall, Vera Lúcia Mores

doi:10.1590/s1517-83822014000100016

Cited by 17 publications

(14 citation statements)

References 29 publications

(69 reference statements)

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“…have intrinsic and acquired resistance to most of the antibiotics used in humans. [14][15][16] Therefore, treatment of enterococcal infections may be difficult. [10] Enterococci are described to be intrinsically resistant to β-lactam antibiotics, with penicillins (ampicillin, amoxicillin/clavulanic acid, penicillin G, methicillin, piperacillin), followed by carbapenems (imipenem) and cephalosporins (cefoperazone, ceftriaxone).…”

Section: Introductionmentioning

confidence: 99%

Prevalence, characterization and antibiotic resistance of enterococci from traditional cheeses in Turkey

Şanlibaba

Şentürk

2018

International Journal of Food Properties

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The aim of the present work is to provide information about Enterococcus strains isolated from traditional Turkish cheese samples in Ankara (Turkey), focusing on their prevalence, phenotypic and genotypic characteristics, and antibiotic resistance. A total of 213 probable enterococcal isolates isolated from 215 samples were identified by phenotypic and genotypic methods. As a result of 16S rDNA sequence analysis, 88 of the 213 enterococci strains were identified as Enterococcus faecium and 125 as Enterococcus faecalis. The E. faecalis strains (58.7%) were identified as the dominant species isolated from cheese samples in Turkey. The 213 Enterococcus strains were tested for susceptibility to 12 different antimicrobial agents. The resistance phenotype were as follow: nalidixic acid (100%), kanamycin (98.6%), rifampicin (78.4%), ampicillin (48.8%), ciprofloxacin (45.5%), erythromycin (18.8%), tetracycline (11.7%), penicillin G (5.6%), chloramphenicol (4.2%), gentamycin (3.8%) and streptomycin (1.4%). None of the strains was resistant to vancomycin. E. faecium strains showed more resistant phenotypes than E. faecalis strains as shown by the antibiotic resistance levels. It was also observed that the resistance of E. faecium and E. faecalis strains against the antibiotics was statistically significant (p ˂ 0.05). In total, 100% of E. faecium and 88.8% of E. faecalis strains were resistant to multiple drugs.

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Section: Introductionmentioning

confidence: 99%

Prevalence, characterization and antibiotic resistance of enterococci from traditional cheeses in Turkey

Şanlibaba

Şentürk

2018

International Journal of Food Properties

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“…In addition, production of hazardous compounds by some enterococcal strains can be toxic in food production (Franz et al, 2001; Choi and Woo, 2013; Camargo et al, 2014). Due to properties of enterococci as both beneficial organisms and nosocomial pathogens, verifying the safety of a novel probiotic strain is of utmost importance.…”

Section: Introductionmentioning

confidence: 99%

Evaluating the Safety of Potential Probiotic Enterococcus durans KLDS6.0930 Using Whole Genome Sequencing and Oral Toxicity Study

Zhan

Evivie

et al. 2018

Front. Microbiol.

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Enterococcus durans KLDS6.0930 has previously been shown to have probiotic potential. However, being a potential clinical pathogen, it becomes necessary to evaluate its safety status for novel potential probiotic use. The purpose of this study is to systematically evaluate the safety of E. durans KLDS6.0930 based on its genomics, phenotypic characteristics and oral toxicity. The complete genome of E. durans KLDS6.0930 was sequenced and analyzed for safety-related genes. Antibiotic susceptibility and the production of harmful metabolites were tested. A 28-day repeated oral dose toxicity test was implemented in rats. In vitro, E. durans KLDS6.0930 was resistant to five antibiotics, with intrinsic resistances to four antibiotics and no identified genes for the last. E. durans KLDS6.0930 was not hemolytic and virulence factors were non-functional in its genome. E. durans KLDS6.0930 produced a small amount of tyramine and phenethylamine; genes encoding tyramine decarboxylase were identified. In addition, genotype and phenotype analyses showed that the strain did not have the ability to generate D-lactic acid, indole, or nitroreductase. In vivo, E. durans KLDS6.0930 did not induce adverse effects on the organs, hematological and serum biochemical parameters, or cecal bacterial populations in the oral toxicity test. These results indicate that E. durans KLDS6.0930 can be safely used as a potential probiotic for human consumption and animal feed.

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“…Enterococci, due to their fermentative potential, were thought to have the capacity when present in food matrices to extend shelf‐life and to contribute to the improvement of both flavor and texture (Dziewit et al., ). However, production of toxic compounds by some of these bacteria raises concerns about the safety of these isolates in food production (Camargo et al., ; Choi & Woo, ; Franz et al., ). Additionally, several studies reported that enterococci can transfer resistance to even more virulent bacteria, such as Staphylococcus aureus (Bellanger et al., ; Durand, Brueckner, Sampadian, Willett, & Belliveau, ).…”

Section: Introductionmentioning

confidence: 99%

Genomic insights into the pathogenicity and environmental adaptability of Enterococcus hirae R17 isolated from pork offered for retail sale

Peng

Wang

et al. 2017

MicrobiologyOpen

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Genetic information about Enterococcus hirae is limited, a feature that has compromised our understanding of these clinically challenging bacteria. In this study, comparative analysis was performed of E. hirae R17, a daptomycin‐resistant strain isolated from pork purchased from a retail market in Beijing, China, and three other enterococcal genomes (Enterococcus faecium DO, Enterococcus faecalis V583, and E. hirae ATCC ™9790). Some 1,412 genes were identified that represented the core genome together with an additional 139 genes that were specific to E. hirae R17. The functions of these R17 strain‐specific coding sequences relate to the COGs categories of carbohydrate transport and metabolism and transcription, a finding that suggests the carbohydrate utilization capacity of E. hirae R17 may be more extensive when compared with the other three bacterial species (spp.). Analysis of genomic islands and virulence genes highlighted the potential that horizontal gene transfer played as a contributor of variations in pathogenicity in this isolate. Drug‐resistance gene prediction and antibiotic susceptibility testing indicated E. hirae R17 was resistant to several antimicrobial compounds, including bacitracin, ciprofloxacin, daptomycin, erythromycin, and tetracycline, thereby limiting chemotherapeutic treatment options. Further, tolerance to biocides and metals may confer a phenotype that facilitates the survival and adaptation of this isolate against food preservatives, disinfectants, and antibacterial coatings. The genomic plasticity, mediated by IS elements, transposases, and tandem repeats, identified in the E. hirae R17 genome may support adaptation to new environmental niches, such as those that are found in hospitalized patients. A predicted transmissible plasmid, pRZ1, was found to carry several antimicrobial determinants, along with some predicted pathogenic genes. These data supported the previously determined phenotype confirming that the foodborne E. hirae R17 is a multidrug‐resistant pathogenic bacterium with evident genome plasticity and environmental adaptability.

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Prevalence and phenotypic characterization of Enterococcus spp. isolated from food in Brazil

Cited by 17 publications

References 29 publications

Prevalence, characterization and antibiotic resistance of enterococci from traditional cheeses in Turkey

Prevalence, characterization and antibiotic resistance of enterococci from traditional cheeses in Turkey

Evaluating the Safety of Potential Probiotic Enterococcus durans KLDS6.0930 Using Whole Genome Sequencing and Oral Toxicity Study

Genomic insights into the pathogenicity and environmental adaptability of Enterococcus hirae R17 isolated from pork offered for retail sale

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