Occurrence of Candida orthopsilosis in Brazilian tomato fruits (Lycopersicum esculentum Mill.)

Robl, Diogo; Thimoteo, S.S.; Souza, Gisela; Beux, Márcia Regina; Dalzoto, Patricia R.; Pinheiro, Rosângela Lameira; Pimentel, Ida Chapaval

doi:10.1590/s1517-83822014000100015

Cited by 13 publications

(5 citation statements)

References 30 publications

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“…For Saccharomycetes, we found a total of 1849 sequences (Table 2, Fig. 4), most of them are from several published works, including taxonomic and phylogenetic studies with new records and species from Brazil [94][95][96][97][98][99][100][101]. For Schizosaccharomycetes, the only sequence recovered (JQ726610) is part of a study with yeasts isolated from Brazilian cocoa box fermentations [102].…”

Section: Discussionmentioning

confidence: 99%

Brazilian fungal diversity represented by DNA markers generated over 20 years

Menolli

Sánchez-García

2019

Braz J Microbiol

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Molecular techniques using fungal DNA barcoding (ITS) and other markers have been key to identifying the biodiversity of different geographic areas, mainly in megadiverse countries. Here, we provide an overview of the fungal diversity in Brazil based on DNA markers of phylogenetic importance generated since 1996. We retrieved fungal sequences of ITS, LSU, SSU, tef1-α, β-tubulin, rpb1, rpb2, actin, chitin synthase, and ATP6 from GenBank using different field keywords that indicated their origin in Brazil. A total of 19,440 sequences were recovered. ITS is the most representative marker (11,209 sequences), with 70.1% belonging to Ascomycota, 18.6% Basidiomycota, 10.2% unidentified, 1.1% Mucoromycota, two sequences of Olpidium bornovanus (Fungi incertae sedis), one sequence of Blastocladiomycota (Allomyces arbusculus), and one sequence of Chytridiomycota (Batrachochytrium dendrobatidis). Considering the sequences of all selected markers, only the phyla Cryptomycota and Entorrhizomycota were not represented. Based on ITS, using a cutoff of 98%, all sequences comprise 3047 OTUs, with the majority being Ascomycota (2088 OTUs) and Basidiomycota (681 OTUs). Previous numbers based mainly on morphological and bibliographical data revealed 5264 fungal species from Brazil, with a predominance of Basidiomycota (2741 spp.) and Ascomycota (1881 spp.). The unidentified ITS sequences not assigned to a higher taxonomic level represent 1.61% of all ITS sequences sampled and correspond to 38 unknown class-level lineages (75% cutoff). A maximum likelihood phylogeny based on LSU illustrates the fungal classes occurring in Brazil.

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Section: Discussionmentioning

confidence: 99%

Brazilian fungal diversity represented by DNA markers generated over 20 years

Menolli

Sánchez-García

2019

Braz J Microbiol

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“…To date, our study is the first to report that C. spaethianum infects P. cyrtonema and causes rhizome rot in the Guizhou Province, China. On the other hand, our study showed that the diseased plants were more enriched in other fungi, such as Candida and Clonostachys , and Candida is recognized as a potential genus that suppresses pathogenic fungi and is used in controlling plant diseases ( 72 , 73 ). Therefore, more work needs to be conducted with further inoculation of P. cyrtonema crops.…”

Section: Discussionmentioning

confidence: 86%

Multiomics Reveals the Effect of Root Rot on Polygonati Rhizome and Identifies Pathogens and Biocontrol Strain

et al. 2022

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“…is species is closely related to C. parapsilosis, commonly described as a commensal in human skin and pathogenic yeast, capable to develop human tissue invasion and damage [20]. Scarce reports describe this yeast associated to fruits of Opuntia stricta [21] and tomatoes [22].…”

Section: Discussionmentioning

confidence: 99%

Yeasts with Fermentative Potential Associated with Fruits of Camu-Camu (Myrciaria dubia, Kunth) from North of Brazilian Amazon

Matos

Andrade-Souza

D’Angelo

et al. 2021

The Scientific World Journal

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Considering the high biotechnological potential of yeasts associated to edible fruits, a screening for these microorganisms, capable of alcoholic fermentation, was performed in ripe fruits of camu-camu (Myrciaria dubia, Kunth). The fruits were collected from north of Brazilian Amazon, in the floodplain of the Cauamé River. Yeasts were isolated, and fermentation capability was evaluated using Durham tubes. Quantitative assays were performed to calculate ethanol yield (g g−1), specific growth rate (h−1), and ethanol productivity (g L−1·h−1). Taxonomic identification was performed by ribosomal gene nucleotide sequence analysis by alignment using BLASTN. A total of fifteen yeast colonies were isolated, and three of them presented promising ability to ferment glucose to ethanol. These isolates were identified as Candida orthopsilosis, Pichia kudriavzevii, and Meyerozyma caribbica. When cultured in broth containing 180 g·L−1 of glucose, M. caribbica CC003 reached 91.7 percent of the maximum theoretical ethanol concentration (84.4 g·L−1), presenting an ethanol yield and productivity of 0.4688 g·g−1 and 0.781 g·L−1·h−1, respectively. These results indicate a promising potential of this isolate for bioprocess applications. This paper is a rare report of C. orthopsilosis with endophytic habit because most of the references indicate it as a human pathogen. Besides this, M. caribbica is a promising fermenter for alcoholic beverages due to its osmotolerance and high ethanol yield. This is the first paper reporting endophytic yeasts associated with fruits of Myrciaria dubia.

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Occurrence of Candida orthopsilosis in Brazilian tomato fruits (Lycopersicum esculentum Mill.)

Cited by 13 publications

References 30 publications

Brazilian fungal diversity represented by DNA markers generated over 20 years

Brazilian fungal diversity represented by DNA markers generated over 20 years

Multiomics Reveals the Effect of Root Rot on Polygonati Rhizome and Identifies Pathogens and Biocontrol Strain

Yeasts with Fermentative Potential Associated with Fruits of Camu-Camu (Myrciaria dubia, Kunth) from North of Brazilian Amazon

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