Biological characteristics and probiotic effect of Leuconostoc lactis strain isolated from the intestine of black porgy fish

Abstract

A strain of lactic acid bacteria, Leuconostoc lactis, was isolated from the intestinal tract of black porgy, Sparus macrocephalus, and identified by conventional biochemical characteristics and 16S rDNA gene sequence analysis. The isolated strain had the ability of bile tolerance and resistance to low pH, and survived well in the trypsinase and pepsin solution. But the highly concentrated dose of trypsinase and pepsin affect the viability of the isolated strain. The isolate was resistant to several antibiotics, including Cephalothin, Ceftriaxone, Imipenem and Tobramycin. The isolate could auto-aggregate itself and coaggregate with other bacteria in vitro. The autoaggregation percentage increased to 23.29% after 20 h of incubation. The percentage of coaggregation were respectively 31.21%, 29.44%, 10.74%, 16.49%, 24.36%, 24.41% and 20.99% for Vibrio parahaemolyticus, Listeria monocytogenes, Escherichia coli O157, Salmonella typhimurium, Shigella, Staphylococcus aureus and Proteusbacillus vulgaris after 20 h incubation of a mixed suspension. The supernatant of the strain inhibited the growth of several pathogens, such as V.parahaemolyticus, Vibrio harveyi, Vibrio alginolyticus, Staphylococcus aureus, Escherichia coli O157, Salmonella typhimurium, Bacillus subtilis, Proteusbacillus vulgaris and Shigella. These results indicated that the isolate, Leuconostoc lactis, might be an attractive candidate for perspectival strain for probiotics in marine aquaculture.

Characterization of Lactic Acid Bacteria Isolated From the Gastrointestinal Tract of a Wild Boar as Potential Probiotics

“…And coaggregation is the intercellular adhesion between different strains, which is also bound up with the ability of interacting with pathogens. Therefore, aggregation may constitute one kind of defense mechanisms of host for anti-infection (52). In this study, five LAB isolated from the same wild boar were observed to have different levels of autoaggregation and coaggregation.…”

Microbial Surface Colonization and Biofilm Development in Marine Environments

“…Although investigations of coaggregation partnerships and their consequences have scarcely been undertaken with marine bacteria (635,636), it is reasonable to hypothesize that coaggregation-based cellcell interactions may play as important a role in marine biofilm formation as in freshwater environments (616,628). According to McCormick et al (628), microbial coaggregation associated with marine particles may help to establish a parsimonious food chain, increasing the energetic potential of metabolites of these resource islands in generally oligotrophic surroundings.…”

Probiotic potential of thermotolerant lactic acid bacteria isolated from “Gari “a cassava-based African fermented food

“…Salmonella enterica serovar Enteritidis, Salmonella enterica serovar Typhimurium, Listeria monocytogenes, Staphylococcus aureus and Bacillus Sp. The ability of inhibiting the growth of pathogenic strains is one of the most important probiotic properties common in lactic acid bacteria [12][13][14][15]. Scapin et al [16] have reported similar results in regard to the action of the probiotic strain Lactobacillus acidophilus LA10 against Salmonella Enteritidis SE86 in mice.…”