Optimization and characterization of alkaline protease and carboxymethyl-cellulase produced by Bacillus pumillus grown on Ficus nitida wastes

Gomaa, Eman Zakaria

doi:10.1590/s1517-83822013005000048

Cited by 32 publications

(17 citation statements)

References 26 publications

(27 reference statements)

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“…In susceptibility testing the largest zones of inhibition were found against S. aureus and the smallest against Aspergillus flavus . These results were in accordance with previously reported antibacterial, antifungal and antiviral activity of biosurfactants (Gomaa ; Borsanyiova et al . ; Jemil et al .…”

Section: Antimicrobial and Antifungal Properties Of Biosurfactantssupporting

confidence: 94%

Microbial biosurfactants: current trends and applications in agricultural and biomedical industries

et al. 2019

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Synthetic surfactants are becoming increasingly unpopular in many applications due to previously disregarded effects on biological systems and this has led to a new focus on replacing such products with biosurfactants that are biodegradable and produced from renewal resources. Microbially derived biosurfactants have been investigated in numerous studies in areas including: increasing feed digestibility in an agricultural context, improving seed protection and fertility, plant pathogen control, antimicrobial activity, antibiofilm activity, wound healing and dermatological care, improved oral cavity care, drug delivery systems and anticancer treatments. The development of the potential of biosurfactants has been hindered somewhat by the myriad of approaches taken in their investigations, the focus on pathogens as source species and the costs associated with large‐scale production. Here, we focus on various microbial sources of biosurfactants and the current trends in terms of agricultural and biomedical applications.

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Section: Antimicrobial and Antifungal Properties Of Biosurfactantssupporting

confidence: 94%

Microbial biosurfactants: current trends and applications in agricultural and biomedical industries

et al. 2019

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“…D r a f t 11 casein, 10.0; and agar, 20.0 (Gomaa 2013). Plates containing this medium were inoculated and incubated as described previously for other extracellular enzymes.…”

Section: Protease Activitymentioning

confidence: 99%

Isolation and characterization ofBacillusspp. strains as potential probiotics for poultry

Peñaloza‐Vázquez

Rayas‐Duarte

2019

Can. J. Microbiol.

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Canadian Journal of Microbiology D r a f t 2 ABSTRACT Probiotics have become one of the potential solutions to global restriction on antibiotic use in food animal production. Bacillus species have been attractive probiotics partially due to their long-term stability during storage. In this study, 200 endospore-forming bacteria isolates were recovered from sourdough and the gastrointestinal tract (GIT) of young broiler chicks. Based on the production of a series of exoenzymes and survivability under stress conditions similar to those in the poultry gastrointestinal tract (GIT), 42 isolates were selected and identified by 16S rRNA gene sequencing. Seven strains with a profile of high enzymatic activities were further evaluated for sporulation efficiency, biofilm formation, compatibility among themselves (Bacillus spp.) and antagonistic effects against three pathogenic bacteria to poultry and human including Enterococcus cecorum, Salmonella enterica, and Shiga Toxin-Producing-Escherichia coli. The strains from sourdough were identified as Bacillus amyloliquefaciens while the ones from the chicks' GIT were Bacillus subtilis. These strains demonstrated remarkable potential as probiotic for poultry.

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“…The protease production recorded for isolates "E," "F," and "G" was 174 U/ml, 143 U/ml, and 134 U/ml, respectively. Maximum growth and enzymes production were recorded after 72 h of incubation [19][20][21]. Effect of incubation period (pH 9.0; 37°C) Fig.…”

Section: Effect Of Incubation Period (Ph 70; 37°c)mentioning

confidence: 94%

Isolation, Purification, and Optimization of Thermophilic and Alkaliphilc Protease Originating From Hot Water Spring Bacteria

Puntambekar

Dake

2017

Asian J Pharm Clin Res

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Objective: The main objective of this study is to investigate the industrial applications of a thermophillic alkaline protease from a hot water spring bacterial isolate "A" and to study its production, optimization, and purification. Methods:The alkaline protease was produced using shake flask studies maintaining a pH of 9.0 and a temperature of 50°C. Optimization studies of the enzyme were carried out using variable pH, temperature, organic carbon, and nitrogen sources followed by purification of the enzyme using DEAE-cellulose ion exchange chromatography technique. Stability of the enzyme was analyzed in the presence of organic solvents and surfactants. The efficiency of the enzyme in the removal of proteinaceous stains in the presence of strong detergents under extreme conditions was assessed. The fibrinolytic activity of the enzyme in dissolving the blood clot was confirmed. Results:The isolated alkaline protease was purified to homogeneity with a 16-fold increase. Media optimization studies revealed that 1% glucose and 1 % casein-induced the production of alkaline protease. The purified enzyme retained stability in the presence of ethanol, methanol, and acetone and surfactants such as 0.5% (w/v) sodium dodecyl sulfate (SDS) and 0.5% (v/v) Triton-X-100. The isolated alkaline protease successfully removed the proteinaceous stains and showed significant results in the dissolution of blood clot. Conclusion:The above experimental results confirm that the isolated enzyme has both thermophilic and alkaliphilic protease properties. Thereby the enzyme finds promising industrial applications even in extreme conditions.

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Optimization and characterization of alkaline protease and carboxymethyl-cellulase produced by Bacillus pumillus grown on Ficus nitida wastes

Cited by 32 publications

References 26 publications

Microbial biosurfactants: current trends and applications in agricultural and biomedical industries

Microbial biosurfactants: current trends and applications in agricultural and biomedical industries

Isolation and characterization ofBacillusspp. strains as potential probiotics for poultry

Isolation, Purification, and Optimization of Thermophilic and Alkaliphilc Protease Originating From Hot Water Spring Bacteria

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