Reduction of Aeromonas hidrophyla biofilm on stainless stell surface by essential oils

Abstract

This study demonstrates the possibility of using sanitizing detergents based on natural products for the elimination and/or reduction of Aeromonas hydrophila biofilm formed on stainless steel surfaces. The goal of this work was to determine the reduction effect of sanitizing detergents containing essential oils of Thymus vulgaris (thyme) and Cymbopogon citratus (lemongrass) on biofilm formed by A. hydrophila on AISI 304 stainless steel coupons, using UHT skimmed milk as substratum. There was adhesion and biofilm formation by A. hydrophila at 28 °C, presenting 7.60 log cfu.cm−2 after the fourth day of cultivation. There was no significant difference between the lemongrass treatment and that of the thyme oil (p < 0.05). However, both treatments significantly reduced the biofilm, differing significantly from the NaOH control (p > 0.05). The treatment with lemongrass solution reduced the biofilm by 4.51 log cfu cm−2 at 25 °C. The thyme detergent also reduced the number of cfu cm−2 by 3.84 log cycles at 25 °C. The use of the lemongrass and thyme solutions efficiently reduced the A. hydrophila biofilm.

Antibacterial potential of phytochemicals alone or in combination with antimicrobials against fish pathogenic bacteria

“…Biofilm formation can be harmful to aquaculture practices, as observed in the high susceptibility of the cage fishing nets to biofilm formation (Ashraf and Edwin 2016). In addition, the EO of C. citratus (with 78% citral) also reduced the A. hydrophila biofilm formed on stainless steel surfaces (Millezi et al 2013). Here, all phytochemicals tested inhibited biofilm formation, displaying a superior activity compared to the antimicrobials tested.…”

“…Biofilms are complex microbiologic ecosystems embedded in matrixes of organic polymers adhered to a surface (Millezi et al 2013). There is an increase in sessile cell resistance to host defences, biocides, antibiotics and various physiochemical agents, leading to persistence and survival even after sanitization processes (Chavant et al 2007).…”

“…It has been described as species has antibiotic resistance genes and also as responsible for ulcerative syndrome in Chinese longsnout catfish (Leiocassis longirostris) (Cai et al 2012;Sreedharan et al 2012). Bacteria of the genus Aeromonas are recognized for their capacity of haemolysis and biofilm formation, factors that interfere in the strain virulence (Millezi et al 2013;Sutili et al 2014). Citrobacter freundii is a member of the family Enterobacteriaceae that has been reported causing cutaneous haemorrhagic rash and renal granulomas in moonfish (Spring springs), gastroenteritis in fingerlings of rainbow trout (Oncorhynchus mykiss), acute haemorrhagic septicaemia in common carp (Cyprinus carpio) and lesions in the gills and kidneys, severe enteritis, and haemorrhagic septicaemia in catfish (Pseudoplatystoma reticulatum) (Sato et al 1982;Karunasagar et al 1992;Svetlana et al 2003;P adua et al 2014).…”

Anti-biofim and Antibacterial Effect of Essential Oils and Their Major Compounds

“…Essential oils and their compounds are known to be active against a wide variety of microorganisms, including Gram-positive and Gram-negative bacteria 7,8,13,16 in both planktonic form 24,26 and biofilm 25,27,30 . Bacteria in biofilm mode of growth have been much more resistant to antibiotics than their planktonic counterparts 21,33 .…”

Aeromonas biofilm on stainless steel: efficiency of commonly used disinfectants

“…More recently, Millezi et al . () also demonstrated the ability of A. hydrophila (strain ATCC 27853) to form biofilm on stainless steel after 48 h of incubation at 28 °C, reaching values of 6.6 log CFU mL −1 .…”