Formation of biofilm by Listeria monocytogenes ATCC 19112 at different incubation temperatures and concentrations of sodium chloride

Lee, Hai Yen; Chai, Lay Ching; Pui, Chai Fung; Mustafa, Shuhaimi; Cheah, Yoke Kqueen; Nishibuchi, Mitsuaki; Radu, Son

doi:10.1590/s1517-83822013005000004

Cited by 19 publications

(12 citation statements)

References 25 publications

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“…The ability to form biofilms may assist its survival in the environment (Ammendolia et al 2014). However, the level of biofilm production depends upon strain, origin and environmental conditions (Barbosa et al 2013;Lee, Chai, et al 2013;Ochiai et al 2014). Though L. monocytogenes is not an established biofilm former than others (Kalmokoff et al 2001), its persistence in the environment makes it as an emerging problem for the food processing industry and other food-associated environments (e.g., farms and retail establishments) (Gilbert et al 2002;Galvao et al 2012;da Silva & De Martinis 2013;Ferreira et al 2014).…”

Section: Veterinary Quarterlymentioning

confidence: 99%

Listeriosis in animals, its public health significance (food-borne zoonosis) and advances in diagnosis and control: a comprehensive review

Dhama

Karthik

Tiwari

et al. 2015

Veterinary Quarterly

113

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Listeriosis is an infectious and fatal disease of animals, birds, fish, crustaceans and humans. It is an important food-borne zoonosis caused by Listeria monocytogenes, an intracellular pathogen with unique potential to spread from cell to cell, thereby crossing blood-brain, intestinal and placental barriers. The organism possesses a pile of virulence factors that help to infect the host and evade from host immune machinery. Though disease occurrence is sporadic throughout the world, it can result in severe damage during an outbreak. Listeriosis is characterized by septicaemia, encephalitis, meningitis, meningoencephalitis, abortion, stillbirth, perinatal infections and gastroenteritis with the incubation period varying with the form of infection. L. monocytogenes has been isolated worldwide from humans, animals, poultry, environmental sources like soil, river, decaying plants, and food sources like milk, meat and their products, seafood and vegetables. Since appropriate vaccines are not available and infection is mainly transmitted through foods in humans and animals, hygienic practices can prevent its spread. The present review describes etiology, epidemiology, transmission, clinical signs, post-mortem lesions, pathogenesis, public health significance, and advances in diagnosis, vaccines and treatment of this disease. Special attention has been given to novel as well as prospective emerging therapies that include bacteriophage and cytokine therapy, avian egg yolk antibodies and herbal therapy. Various vaccines, including advances in recombinant and DNA vaccines and their modes of eliciting immune response, are also discussed. Due focus has also been given regarding appropriate prevention and control strategies to be adapted for better management of this zoonotic disease.

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Section: Veterinary Quarterlymentioning

confidence: 99%

Listeriosis in animals, its public health significance (food-borne zoonosis) and advances in diagnosis and control: a comprehensive review

Dhama

Karthik

Tiwari

et al. 2015

Veterinary Quarterly

113

View full text Add to dashboard Cite

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“…Formation of L. monocytogenes biofilms can occur with various counts and under on various surfaces, such as on Buna-N rubber (23), at a level of 2.1 log CFU/ cm 2 on 0.8% minimal NaCl medium (24), and on glass and steel surfaces (25). The experimental conditions of this study favored formation of a biofilm on stainless steel after 48 h. Serotype 1/2a is commonly isolated from food so emphasis on this serotype can deepen our understanding of cross contamination of foods.…”

Section: Resultsmentioning

confidence: 99%

Transfer of Listeria monocytogenes between abiotic surfaces under different weights

Lee¹,

Chai²,

Mahyudin³

et al. 2014

Food Sci Biotechnol

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Cross contamination of Listeria monocytogenes from two different surfaces (plastic wrappers and stainless steel coupons) was simulated using a series of different weights. Enumeration of transfer was based on surface bacterial counts of biofilm stained surfaces. Direct contact between 2 surfaces has a constant rate of transfer that is independent of the pressure applied. This is the first report on the study of cross contamination between surfaces using pressure to illustrate transfer of bacteria in a food processing line.

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“…Moreover, subtypes from the same cluster had a close biofilm forming ability, which suggests a possible association between the genetic subtype and the amount of biofilm formed. There have been few studies that contradicted this theory but the relationship between biofilm formation and subtypes should be further investigated [29].…”

Section: Discussionmentioning

confidence: 99%

Genotypic and virulence characteristics of Listeria monocytogenes recovered from food items in Lebanon

Haidar-Ahmad

Kissoyan

Fadlallah

et al. 2016

J Infect Dev Ctries

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Introduction: Listeria monocytogenes is the agent of listeriosis, a life threatening foodborne disease for immunocompromised patients and pregnant women. This bacterium is not routinely screened for in Lebanon and there is lack of data about the prevalent strains and their potential pathogenicity. To that purpose, this study was undertaken to characterize L. monocytogenes from various food products, by assessing the in vitro biofilm forming ability, detecting their virulence potential, and characterizing them at the strain level. Methodology: Fifty-nine isolates were obtained from the Lebanese Agriculture Research Institute (LARI). They were collected in 2012-2013 from local and imported food products in the Lebanese market. Biofilm formation was measured using the Microtiter Plate Assay. PCR amplification was performed for three main virulence genes; hly, actA, and inlB. Pulsed field gel electrophoresis (PFGE) and BIONUMERICS analysis were carried out. Results: Lebanese isolates from cheese and raw meat showed higher biofilm formation than imported and Lebanese seafood isolates. A total of 100% of the isolates were PCR positive for hly and actA genes and 98.3% for inlB gene. PFGE analysis demonstrated the prevalence of 13 different subtypes with 100% similarity. Detected subtypes were grouped into 6 clusters of 90% genomic similarity. Clustered subtypes were particular to the country of origin. Conclusion: This study highlights the presence of L. monocytogenes in the Lebanese food market with high pathogenic potential and stresses the importance of enhanced surveillance and the implementation of strict regulations on local and imported food. Future investigations may be conducted on a larger food selection.

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Formation of biofilm by Listeria monocytogenes ATCC 19112 at different incubation temperatures and concentrations of sodium chloride

Cited by 19 publications

References 25 publications

Listeriosis in animals, its public health significance (food-borne zoonosis) and advances in diagnosis and control: a comprehensive review

Listeriosis in animals, its public health significance (food-borne zoonosis) and advances in diagnosis and control: a comprehensive review

Transfer of Listeria monocytogenes between abiotic surfaces under different weights

Genotypic and virulence characteristics of Listeria monocytogenes recovered from food items in Lebanon

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