Methylobacterium-plant interaction genes regulated by plant exudate and quorum sensing molecules

Dourado, Manuella Nóbrega; Bogas, Andréa Cristina; Pomini, Armando M.; Andreote, Fernando Dini; Quecine, Maria Carolina; Marsaioli, Anita J.; Araújo, Welington Luiz

doi:10.1590/s1517-83822013000400044

Cited by 41 publications

(26 citation statements)

References 54 publications

(75 reference statements)

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“…The biofilm formation is regulated by bacterial density and quorum sensing (QS) molecules in several bacteria, such as N-acyl-homoserine lactones (AHLs) for Methylobacterium spp. These molecules were already reported in M. mesophilicum SR1.6/6 (Pomini et al 2009) and the role during plant colonization was described by Dourado et al (2013), whose observed that in the presence of this AHL, M. mesophilicum SR1.6/6 up-regulated genes related to plant-bacteria interaction, including mxaF (methanol metabolism), patatin (secretion system), acdS (ACC deaminase) and ctrI (carotenoid biosynthesis). These molecules were already reported in M. mesophilicum SR1.6/6 (Pomini et al 2009) and the role during plant colonization was described by Dourado et al (2013), whose observed that in the presence of this AHL, M. mesophilicum SR1.6/6 up-regulated genes related to plant-bacteria interaction, including mxaF (methanol metabolism), patatin (secretion system), acdS (ACC deaminase) and ctrI (carotenoid biosynthesis).…”

Section: Introductionsupporting

confidence: 66%

“…70 % ethanol for 1 min, sodium hypochlorite solution (1.5 % available ClO 3 ) for 2 min, 70 % ethanol for 1 min followed by two rinses in sterile distilled water], as previously reported (Dourado et al 2013). 70 % ethanol for 1 min, sodium hypochlorite solution (1.5 % available ClO 3 ) for 2 min, 70 % ethanol for 1 min followed by two rinses in sterile distilled water], as previously reported (Dourado et al 2013).…”

Section: Methodsmentioning

confidence: 66%

“…The selection of the reference gene to be used as a normalizer was decided by testing the Ct values obtained after reactions with two different primer sets, one for the recA gene and another for the zwf gene (Holmes et al 2005;Dourado et al 2013). The zwf presented the lowest DCt and the higher amplification efficiency, which was then selected as the normalizer for gene expression analyses.…”

Section: Real Time Qpcr Analysesmentioning

confidence: 99%

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Genes related to antioxidant metabolism are involved in Methylobacterium mesophilicum-soybean interaction

Araújo

Santos

Dini‐Andreote

et al. 2015

Antonie van Leeuwenhoek

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The genus Methylobacterium is composed of pink-pigmented methylotrophic bacterial species that are widespread in natural environments, such as soils, stream water and plants. When in association with plants, this genus colonizes the host plant epiphytically and/or endophytically. This association is known to promote plant growth, induce plant systemic resistance and inhibit plant infection by phytopathogens. In the present study, we focused on evaluating the colonization of soybean seedling-roots by Methylobacterium mesophilicum strain SR1.6/6. We focused on the identification of the key genes involved in the initial step of soybean colonization by methylotrophic bacteria, which includes the plant exudate recognition and adaptation by planktonic bacteria. Visualization by scanning electron microscopy revealed that M. mesophilicum SR1.6/6 colonizes soybean roots surface effectively at 48 h after inoculation, suggesting a mechanism for root recognition and adaptation before this period. The colonization proceeds by the development of a mature biofilm on roots at 96 h after inoculation. Transcriptomic analysis of the planktonic bacteria (with plant) revealed the expression of several genes involved in membrane transport, thus confirming an initial metabolic activation of bacterial responses when in the presence of plant root exudates. Moreover, antioxidant genes were mostly expressed during the interaction with the plant exudates. Further evaluation of stress- and methylotrophic-related genes expression by qPCR showed that glutathione peroxidase and glutathione synthetase genes were up-regulated during the Methylobacterium-soybean interaction. These findings support that glutathione (GSH) is potentially a key molecule involved in cellular detoxification during plant root colonization. In addition to methylotrophic metabolism, antioxidant genes, mainly glutathione-related genes, play a key role during soybean exudate recognition and adaptation, the first step in bacterial colonization.

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Section: Introductionsupporting

confidence: 66%

Section: Methodsmentioning

confidence: 66%

Section: Real Time Qpcr Analysesmentioning

confidence: 99%

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Genes related to antioxidant metabolism are involved in Methylobacterium mesophilicum-soybean interaction

Araújo

Santos

Dini‐Andreote

et al. 2015

Antonie van Leeuwenhoek

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“…Quorum sensing signals are involved in plant colonization by Methylobacterium (Dourado et al, 2014). Metagenomic analysis revealed that three quorum sensing systemsautoinducer-2 system, the diffusible signal factor system and N-acyl homoserine lactone (AHL) system are present in endophytic rice microbiome (Sessitsch et al, 2012).…”

Section: Occurrence and Diversity Of Bacterial Endophytes In Agricultmentioning

confidence: 99%

Bacterial endophytes in agricultural crops and their role in stress tolerance: a review

Miliūtė

Buzaitė

Baniulis

et al. 2015

Zemdirbyste-Agriculture

197

112

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Bacterial endophytes are a class of endosymbiotic microorganisms widespread among plants that colonize intercellular and intracellular spaces of all plant compartments and do not cause plant disease or significant morphological changes. Plant and endophytic bacteria association includes vast diversity of bacterial taxa and plant hosts and in this review we present an overview of taxonomic composition of endophytes identified in common agricultural crops. Further, during the last decade, new aspects of the microbial diversity have emerged with application of new metagenomic analysis methods in studies of bacterial endophytes. Endophytic bacteria community structure is influenced by plant genotype, abiotic and biotic factors such as environment conditions, microbe -microbe interactions and plant -microbe interactions. Agricultural practices, such as soil tillage, irrigation, use of pesticides and fertilizers have a major effect on function and structure of soil and endophytic microbial populations. Therefore, the use of agricultural practices that maintain natural diversity of plant endophytic bacteria is becoming an important element of sustainable agriculture that could ensure plant productivity and quality of agricultural production. The diverse endophytic microbial communities play integral and unique role in the functioning of agroecosystems. Endophytic bacteria have been shown to have several beneficial effects on their host plant, including growth promoting activity, modulation of plant metabolism and phytohormone signalling that leads to adaptation to environmental abiotic or biotic stress. Use of endophytic bacteria presents a special interest for development of agricultural applications that ensure improved crop performance under cold, draught or contaminated soil stress conditions or enhanced disease resistance.

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“…The absence of these fungi may result in the emergence of X. fastidiosa as a pathogen (Aldrich et al ., 2015). Additionally, studies (Dourado et al ., 2013, 2015; Lacava et al ., 2004, 2006a) have shown that the development of CVC symptoms in citrus plants could be a result of an unbalanced endophytic population, including Methylobacterium spp. and X. fastidiosa .…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

The diversity of citrus endophytic bacteria and their interactions with Xylella fastidiosa and host plants

2016

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The bacterium Xylella fastidiosa is the causal agent of citrus variegated chlorosis (CVC) and has been associated with important losses in commercial orchards of all sweet orange [Citrus sinensis (L.)] cultivars. The development of this disease depends on the environmental conditions, including the endophytic microbial community associated with the host plant. Previous studies have shown that X. fastidiosa interacts with the endophytic community in xylem vessels as well as in the insect vector, resulting in a lower bacterial population and reduced CVC symptoms. The citrus endophytic bacterium Methylobacterium mesophilicum can trigger X. fastidiosa response in vitro, which results in reduced growth and induction of genes associated with energy production, stress, transport, and motility, indicating that X. fastidiosa has an adaptive response to M. mesophilicum. Although this response may result in reduced CVC symptoms, the colonization rate of the endophytic bacteria should be considered in studies that intend to use this endophyte to suppress CVC disease. Symbiotic control is a new strategy that uses symbiotic endophytes as biological control agents to antagonize or displace pathogens. Candidate endophytes for symbiotic control of CVC must occupy the xylem of host plants and attach to the precibarium of sharpshooter insects to access the pathogen. In the present review, we focus on interactions between endophytic bacteria from sweet orange plants and X. fastidiosa, especially those that may be candidates for control of CVC.

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Methylobacterium-plant interaction genes regulated by plant exudate and quorum sensing molecules

Cited by 41 publications

References 54 publications

Genes related to antioxidant metabolism are involved in Methylobacterium mesophilicum-soybean interaction

Genes related to antioxidant metabolism are involved in Methylobacterium mesophilicum-soybean interaction

Bacterial endophytes in agricultural crops and their role in stress tolerance: a review

The diversity of citrus endophytic bacteria and their interactions with Xylella fastidiosa and host plants

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