Detection of CDT toxin genes in Campylobacter spp. strains isolated from broiler carcasses and vegetables in São Paulo, Brazil

Carvalho, Aline Feola de; Silva, Daniela Martins da; Azevêdo, Sérgio Santos de; Piatti, Rosa Maria; Genovez, Margareth Élide; Scarcelli, E.

doi:10.1590/s1517-83822013000300005

Cited by 29 publications

(22 citation statements)

References 33 publications

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“…In this study, the cdtB gene was detected in all (24, 100%) Campylobacter isolates while cdtA and cdtC genes were detected in 83.3% and 75% of the isolates, respectively. These results concur with the findings of studies by Gonzalez-Hein et al 36 , Carvalho et al 37 and Casabonne et al 38 who detected cdtB genes in all Campylobacter species isolated from various sources. In contrast, Montwedi and Ateba 25 reported relatively lower detection rates of cdtA (18/50, 36%) and cdtC (9/50, 18%) genes from chicken meat samples.…”

Section: Pcr Detection Of Virulence Gene Determinants Of Campylobactesupporting

confidence: 92%

Detection of virulent thermophilic Campylobacter species in communal chickens

Bissong

Ateba

2019

S. Afr. J. Sci.

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Campylobacter is a foodborne pathogen found in the gut of poultry and other animals, which thereby act as reservoirs for human infection. Campylobacter produces various virulence factors which contribute to the establishment of disease. The aim of this study was to detect virulence genes in Campylobacter isolated from chicken faeces. A total of 408 poultry cloacal samples were analysed through culture, PCR and DNA sequencing. Campylobacter were detected in 24 (5.9 %) of the samples, of which 21 were C. jejuni while 3 were C. coli. The cdtB gene was detected in all Campylobacter isolates while cdtA and cdtC genes were detected in 20 (83.3%) and 18 (75.0%) of the isolates, respectively. The cadF gene was detected in 18 (85.7%) C. jejuni but not in C. coli. Eight genotypes designated G1 to G8 were detected among the isolates. Genotypes G1 and G7 possessed all three cdt genes, a potential for producing functionally active toxins. A large proportion (70.8%) of the isolates possessed both genes for toxin production and adhesion. The high prevalence of cdt and cadF genes in Campylobacter species in this study indicates the pathogenic potential of the isolates. There is a need to implement control measures to curb the spread of these virulent strains.

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Section: Pcr Detection Of Virulence Gene Determinants Of Campylobactesupporting

confidence: 92%

Detection of virulent thermophilic Campylobacter species in communal chickens

Bissong

Ateba

2019

S. Afr. J. Sci.

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“…cdtA, cdtB, and cdtC encode for a protein that releases cytotoxins that damage the nuclear DNA. Cell cycle arrest and cell death could also be caused by the translocation of the cdtB gene to the nucleus of the cells inducing genotoxic effects on the host DNA [22]. A high prevalence of cdtA (61%) and cdtC (90%) was observed in C. coli isolates only in retail meat products.…”

Section: Virulence Factorsmentioning

confidence: 99%

Characterisation of Campylobacter spp. Isolated from Poultry in KwaZulu-Natal, South Africa

et al. 2020

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This study investigated the antibiotic resistance, virulence profiles, and clonality of Campylobacter jejuni and Campylobacter coli isolated from an intensive poultry farming system in KwaZulu-Natal, South Africa. Following ethical approval, samples were collected over six weeks using the farm-to-fork approach. Campylobacter spp. were identified using culture, confirmed and differentiated to species level by PCR, and subjected to antibiotic susceptibility testing. Selected antibiotic resistance (and mutations) and virulence genes were screened by PCR and confirmed by DNA sequencing. Genetic relatedness amongst the isolates was ascertained using pulsed-field gel electrophoresis. In all, 105 isolates were confirmed as belonging to both Campylobacter coli (60; 57%) and C. jejuni (45; 43%). The highest resistance was recorded against erythromycin and clindamycin. The gyrA mutation, A20175C/A2074G point mutation, tet(O), and cmeB, all associated with antibiotic resistance, were detected. All the virulence genes (pldA, ciaB, cdtA, cdtB, cdtC, dnaJ, except for cadF) were also detected. Isolates were grouped into five pulsotypes displaying 85% similarity, irrespective of their resistance profiles. The numerous permutations of clonality, antibiotic resistance, and virulence profiles evident in Campylobacter spp. pose a challenge to food safety and necessitate a comprehensive understanding of the molecular epidemiology of this organism to decrease its spread in the food chain.

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“…Lettuce and spinach are described in the international literature as the main vegetable sources of human infection by Campylobacter spp. [ 1 , 16 , 25 , 26 ]. An increased interest in the campylobacteriosis risk assessment of raw vegetables is driven by several outbreaks of infections caused by consumption of fresh produce, such as leafy vegetables and salads [ 14 ], lettuce [ 15 ], and sprout and cabbage [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

The prevalence of Campylobacter spp. in vegetables, fruits, and fresh produce: a systematic review and meta-analysis

et al. 2018

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There are a number of reports indicating correlation between outbreaks of campylobacteriosis and the consumption of raw vegetables. This study is a meta-analysis on the prevalence of Campylobacter in fresh vegetables and fruits without any location limitation, which was performed through a documented review of the available resources. Relevant literature was reviewed by trained reviewers, who examined the results for the inclusion of articles in the meta-analysis. The prevalence of Campylobacter in raw vegetables, the sample source, the Campylobacter species, and the method of detection were extracted. The prevalence of Campylobacter in vegetables, fruits, and fresh produce were estimated to be 0.53%. Analysis of the various sample groups initially showed that the bean and sprouts group was the vegetable with the highest prevalence of Campylobacter (11.08%). The rate of contamination was higher when both the molecular and conventional methods were employed. The highest prevalence of Campylobacter was found in Asia (33.4%). Despite the low prevalence, consumption of raw vegetables is inherently risky because no treatment is used to inactivate the pathogens. Therefore, proper sanitation methods are recommended to treat the raw products.

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Detection of CDT toxin genes in Campylobacter spp. strains isolated from broiler carcasses and vegetables in São Paulo, Brazil

Cited by 29 publications

References 33 publications

Detection of virulent thermophilic Campylobacter species in communal chickens

Detection of virulent thermophilic Campylobacter species in communal chickens

Characterisation of Campylobacter spp. Isolated from Poultry in KwaZulu-Natal, South Africa

The prevalence of Campylobacter spp. in vegetables, fruits, and fresh produce: a systematic review and meta-analysis

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