Effect of sub-minimum inhibitory concentrations of ciprofloxacin, amikacin and colistin on biofilm formation and virulence factors of Escherichia coli planktonic and biofilm forms isolated from human urine

Wojnicz, Dorota; Tichaczek−Goska, Dorota

doi:10.1590/s1517-83822013000100037

Cited by 30 publications

(24 citation statements)

References 28 publications

(33 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The motilities of the bacterial cells were determined according to Wojnicz and Tichaczek-Goska (2013). Bacterial strains were incubated for 24, 48 and 72 h at 37°C with and without eugenol and linalool.…”

Section: Motility Testmentioning

confidence: 99%

Eugenol and linalool: Comparison of their antibacterial and antifungal activities

El-Baky¹,

Hashem²

2016

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

Antimicrobial resistance is one of the greatest threats to human health. Alternatives to antimicrobials are needed to combat the rise of bacterial resistance. Essential oils (EOs) and their components are potential sources of new antimicrobials. The present study was conducted to evaluate the antibacterial and antifungal activities of two components of EOs. The antimicrobial mechanisms of eugenol and linalool were investigated against five bacterial strains and four Candida strains. Broth macrodilution method was used to compare the antibacterial and anticandidal activities of the two compounds. They exhibited antimicrobial activity against all tested strains. Germ tube formation by Candida albicans was investigated and it was found that it was completely inhibited at sub-MICs of eugenol while linalool showed minor activity compared to eugenol. Time kill kinetic studies indicated that eugenol was highly toxic to all bacterial and fungal strains within 2.5 h of exposure. Absorbance of intracellular constituents was measured at 260 nm. Only eugenol was highly effective toward lysis and cellular content leakage compared to control drugs. In addition, scanning electron microscopy (SEM) was used to charactize the effect of the two components on cell morphology and showed that both compounds induced cellular deformity of nearly all tested cells. Also, it was found that only eugenol inhibited the Beta-lactamase production and urease activity and it diminished bacterial motility of all tested bacterial strains. These results indicate that eugenol and linalool are effective antimicrobial agents and both antibacterial and antifungal activities of linalool were much weaker than that of eugenol.

show abstract

Section: Motility Testmentioning

confidence: 99%

Eugenol and linalool: Comparison of their antibacterial and antifungal activities

El-Baky¹,

Hashem²

2016

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

show abstract

“…Bacterial culture was inoculated into the tube with Motility Agar. Control cultures contained no drugs [30].…”

Section: Effect Of Mics Of the Tested Agents On Motility Of Ps Aerugmentioning

confidence: 99%

Antibacterial, Anti-biofilm Activity of Some Non-steroidal Anti-Inflammatory Drugs and N-acetyl Cysteine against Some Biofilm Producing Uropathogens

Mohsen¹,

Gomaa²,

Mohammed³

et al. 2015

AJEID

View full text Add to dashboard Cite

Ureteral catheters are indispensable devices used in the management of ureteral obstruction. Although the stent is essential for treatment, it also has complications, which are encrustation, stone formation and biofilm formation. Biofilm infections result in a complication in the course of treatment, increasing the length of patients stay in hospital and overall cost. Catheter-associated infections are difficult to be treated with antibiotics and there is a need to change catheters due to the formation of biofilm on their surfaces. In this study, In this study, we examine the effect of some of prescribed drugs as NSAIDs and N-acetylcysteine on the adherence of S. aureus, K. pneumoniae, P. aeruginosa and Proteus mirabilis on the surface of catheters, and their effects on the preformed mature biofims. Also, we determine their antibacterial activity. The results showed that the tested agents had good antibacterial activity, a significant effect on the inhibition of adherence of the tested strains to plastic surfaces and a high disruptive effect on mature biofilms. In conclusion, the tested drugs can be used in the treatment of catheterassociated infections.

show abstract

“…It has also been shown that biofilm production is among the bacterial traits affected by sub-MICs of antibiotics. While some studies reported inhibitory effects of sub-MICs of antibiotics on biofilm formation [11], several studies carried out over the past decade showed the opposite effect. That is, it has convincingly been shown that sub-MICs of some antibiotics actually induce biofilm formation in different bacteria [12,13].…”

Section: Introductionmentioning

confidence: 99%

Influence of subinhibitory antibiotic concentration on Streptococcus pyogenes adherence and biofilm production

Šmitran

Vuković

Opavski

et al. 2018

AMicr

View full text Add to dashboard Cite

In this study, the focus was on the effects of sub-MICs of the antibiotics on adherence, hydrophobicity, and biofilm formation by two groups of Streptococcus pyogenes strains, which were responsible for different clinical cases. The aim of this study was to explore the effects of sub-MICs of penicillin, ceftriaxone, erythromycin, and clindamycin on adherence, surface hydrophobicity, and biofilm biomass in two selected collections of group A streptococcus (GAS): strains isolated from carriers (CA) and strains isolated from patients with tonsillopharyngitis (TPh). Isolates were tested for hydrophobicity to xylene, adherence, and biofilm production in uncoated microtiter plates before and after treatment with 1/2 and 1/4 MICs of antibiotics. Penicillin reduced adherence and biofilm production in TPh strains, whereas ceftriaxone diminished adherence and biofilm formation in CA group. On the contrary, clindamycin enhanced adherence and biofilm production in both groups of strains. Erythromycin did not significantly alter adherence, but triggered biofilm production in both groups of isolates. Hydrophobicity of both groups of strains was significantly reduced after exposure to all antibiotics. Beta-lactams displayed anti-biofilm activity; penicillin diminished both adherence and biofilm production in TPh strains, whereas ceftriaxone reduced it in strains isolated from CA.

show abstract

Effect of sub-minimum inhibitory concentrations of ciprofloxacin, amikacin and colistin on biofilm formation and virulence factors of Escherichia coli planktonic and biofilm forms isolated from human urine

Cited by 30 publications

References 28 publications

Eugenol and linalool: Comparison of their antibacterial and antifungal activities

Eugenol and linalool: Comparison of their antibacterial and antifungal activities

Antibacterial, Anti-biofilm Activity of Some Non-steroidal Anti-Inflammatory Drugs and N-acetyl Cysteine against Some Biofilm Producing Uropathogens

Influence of subinhibitory antibiotic concentration on Streptococcus pyogenes adherence and biofilm production

Contact Info

Product

Resources

About