Comparison of phenotypic and molecular tests to identify lactic acid bacteria

Moraes, Paula Mendonça; Perin, Luana Martins; Júnior, Abelardo Silva; Nero, Luís Augusto

doi:10.1590/s1517-83822013000100015

Cited by 60 publications

(34 citation statements)

References 12 publications

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“…In this study, biochemical test was used to identify bacteria species based on differences of biochemical activities of bacteria. It can be carried out conventionally or through commercial identification kit such as API system (Moraes et al, 2013). Commercial identification kit has been widely used as it is fast and its software databases mainly contain clinically important bacteria.…”

Section: Discussionmentioning

confidence: 99%

Description of novel marine bioflocculant-producing bacteria isolated from biofloc of Pacific whiteleg shrimp,Litopenaeus vannameiculture ponds

et al. 2018

Preprint

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19Description of marine bioflocculant-producing bacteria isolated from biofloc of 20 Pacific whiteleg shrimp, Litopenaeus vannamei culture ponds was prompted to 21 explore the bacteria that enhanced bioflocculation process in aquaculture wastewater 22 treatment. Certain marine bacteria were potentially secreted extracellular polymeric 23 substances (EPS) which response to the physiological stress encountered in the natural 24 environment that can act as bioflocculants. This study aimed to identify marine 25 2 bioflocculant-producing bacteria isolated from biofloc; to evaluate their flocculating 26 activities; and to characterize their protein in EPS. Phenotypic and genotypic 27 identification of the bacteria including morphological and molecular approaches were 28 employed, while their flocculating activities were examined via Kaolin clay 29 suspension method and statistically analyzed. The EPS that acted as bioflocculants 30 were extracted using cold ethanol precipitation method. Protein concentration was 31 determined by Bradford assay and protein profiling was finally completed with 32 Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) method. 33 Six species of marine bacteria known as Halomonas venusta, Bacillus cereus, Bacillus 34 subtilis, Bacillus pumilus, Nitratireductor aquimarinus and Pseudoalteromonas sp. 35 were successfully identified as bioflocculant-producing bacteria. The highest 36 flocculating activity was exhibited by Bacillus cereus at 93%, while Halomonas 37venusta showed the lowest record at 59%. All bioflocculant-producing bacteria 38 species showed different protein concentration that ranged between 1.377 µg/mL to 39 1.455 µg/mL. Several protein bands with different molecular weight that ranged 40 between 16 kDa to 100 kDa were observed. This study revealed that all the identified 41 bacteria species have high potential characteristics to initiate aquaculture wastewater 42 treatment and may play important roles in bioflocculation process. 43 Importance: 46 Six species of marine bacteria isolated from biofloc of Pacific whiteleg shrimp, 47 Litopenaeus vannamei culture ponds were identified as bioflocculant-producing 48 bacteria. Among those six species, Bacillus cereus, Bacillus pumilus, Nitratireductor 49 3 aquimarinus and Pseudoalteromonas sp. were highly potential to be used as booster 50 for rapid formation of biofloc due to their high flocculating activities. Protein content 51 in EPS of novel marine biofocculant-producing bacteria has beneficial consequences 52 on degradation process of organic substances, denitrification of wastes and ions 53 elimination from aquaculture wastes.54

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Section: Discussionmentioning

confidence: 99%

Description of novel marine bioflocculant-producing bacteria isolated from biofloc of Pacific whiteleg shrimp,Litopenaeus vannameiculture ponds

et al. 2018

Preprint

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“…Furthermore, the inhibitory effect on the growth of several enteropathogenic bacteria is likely associated with the antimicrobial compounds produced by lactic acid bacteria, such as bacteriocin and lactic, acetic and other short-chain organic acids, which are responsible for a reduction in the intestinal pH (Servin & Coconnier 2003, Cheng et al 2003, Varalakshmi et al 2013. Lactic acid represents the main antimicrobial compound present in cultures of lactic acid bacteria (Earnshaw 1992, Navarro et al 2000, Todorov & Dicks 2005, Rossland et al 2005, Moraes et al 2013. Weak acids possess higher antimicrobial activity than strong acids, which ionize completely in an aqueous solution (Axe & Bailey 1995).…”

Section: Introductionmentioning

confidence: 99%

Effect of Lactobacillus sp. isolates supernatant on Escherichia coli O157:H7 enhances the role of organic acids production as a factor for pathogen control

et al. 2015

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RESUMO.-[Efeito do sobrenadante de isolados de Lactobacillus sp. sobre Escherichia coli O157:H7 reforça o papel da produção de ácidos orgânicos como fator de controle patogênico.] Muitas tentativas têm sido feitas para se estabelecer o controle de patógenos de origem alimentar através do uso de estirpes de Lactobacillus e dos seus produtos de metabolismo, com sucesso sendo sucedido em várias situações. O objetivo deste trabalho foi investigar o efeito antagônico do sobrenadante de culturas de oitosobre Escherichia coli amostra O157:H7. Os efeitos inibidores de culturas puras e de dois "pools" de cultura de Lactobacillus sobre o crescimento da bactéria foram avaliados através do método de inibição em ágar e através do monitoramento da turbidez da cultura bacteriana. A atividade antimicrobiana foi confirmada para Lactobacillus reuteri e Lactobacillus delbrueckii subsp. delbrueckii e para o "pool" de bactérias acido-láctica. O sobrenadante neutralizado do "pool" de Lactobacillus exerceu uma atividade antimicrobiana mais elevada do que aquela das estirpes individuais. Além disso, ácido D-láctico e ácido acético foram produzidos durante o crescimento dos Lactobacillus estudados. TERMOS DE INDEXAÇÃO:Lactobacillus, antagonismo, Escherichia coli O157:H7, ácido orgânico.

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“…Because many LAB have similar nutritional and growth requirements, biochemical-based methodologies for identification might not be conclusive in many cases. The conventional microbiological methods for bacterial identification are based on morphological and physiological characteristics such as Gram staining, cell shape, spore formation, enzyme production and the fermentation of different carbohydrates (Moraes et al, 2013). Phenotypic identification and biochemical tests have been considered for a long time the conventional procedure for routine identification of bacteria.…”

Section: Introductionmentioning

confidence: 99%

Biochemical and molecular identification of lactic acid bacteria isolated from camel milk in Tunisia

Fguiri

Ziadi

Atigui

et al. 2015

Emir. J. Food Agric

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Comparison of phenotypic and molecular tests to identify lactic acid bacteria

Cited by 60 publications

References 12 publications

Description of novel marine bioflocculant-producing bacteria isolated from biofloc of Pacific whiteleg shrimp,Litopenaeus vannameiculture ponds

Description of novel marine bioflocculant-producing bacteria isolated from biofloc of Pacific whiteleg shrimp,Litopenaeus vannameiculture ponds

Effect of Lactobacillus sp. isolates supernatant on Escherichia coli O157:H7 enhances the role of organic acids production as a factor for pathogen control

Biochemical and molecular identification of lactic acid bacteria isolated from camel milk in Tunisia

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