Pseudorabies virus can be classified into five genotypes using partial sequences of UL44

Abstract

Suid herpesvirus 1 (SuHV-1) is the causative agent of pseudorabies (PR), a disease of great importance due to the huge losses it causes in the swine industry. The aim of this study was to determine a method for genotyping SuHV-1 based on partial sequences of the gene coding for glycoprotein C (gC) and to elucidate the possible reasons for the variability of this region. A total of 109 gCsequences collected from GenBank were divided into five major groups after reconstruction of a phylogenetic tree by Bayesian inference. The analysis showed that a portion of gC (approximately 671 bp) is under selective pressure at various points that coincide with regions of protein disorder. It was also possible to divide SuHV-1 into five genotypes that evolved under different selective pressures. These genotypes are not specific to countries or continents, perhaps due to multiple introduction events related to the importation of swine.

Genomic characterization of emergent pseudorabies virus in China reveals marked sequence divergence: Evidence for the existence of two major genotypes

“…The gC gene has long been used for phylogenetic analysis of PRV strains mainly because the sequence is prone to variation and because numerous gC sequences have been deposited in public databases (Goldberg et al, 2001;Ishikawa et al, 1996). The partial sequence of gC that is approximately 600 bp away from the 5 0 terminus of the sequence has been used for phylogenetic analysis in many previous studies (Fonseca et al, 2010(Fonseca et al, , 2012Goldberg et al, 2001). As described above, a phylogenetic tree was constructed using the 5 0 -terminal 643 bp of the PRV gC gene.…”

Characterisation of pseudorabies virus in domestic pigs and wild boars in Croatia

“…A correct diagnosis can be obtained by PrV isolation or by molecular detection of the viral DNA, but ELISA tests are highly sensitive and specific, and the PrV seroprevalence thus determined gives us valuable information on the circulating virus strains and may help to identify epidemiological links (Belák, 2005;Fonseca et al, 2012).…”

Different methods of real-time PCR for detection of pseudorabies virus

“…Virus isolates used in this research were the standard strain Shope, vaccine strain Bartha and fourteen Brazilian SuHV-1 isolates (four genotype I and ten genotype II) previously characterized by FONSECA JR. et al (2010a) andFONSECA JR et al (2012). Viruses were grown in PK15 cells and DNA extracted as described by FONSECA JR et al (2010b) (Group 1).…”

“…The inclusion tests were conducted using fourteen SuHV-1 isolates from two different genetic groups and vaccine strain Bartha (FONSECA JR et al, 2012). Exclusion tests were performed with classical swine fever virus, African swine fever virus, bovine herpesvirus 1, porcine parvovirus, porcine circovirus 2 and Streptococcus suis.…”