Antimycobacterial activity of a Brevibacillus Laterosporus strain isolated from a moroccan soil

Abstract

The treatment of tuberculosis has become more difficult with the worldwide spread of multidrug-resistant (MDR) and extensively drug-resistant (XDR) strains of Mycobacterium tuberculosis. Moreover, the prevalence of human disease caused by atypical mycobacteria has also increased in the past two decades and has further complicated the problem of the treatment of mycobacterial infections. It is therefore urgent to develop new highly active molecules against these bacteria. The present study reports the isolation from a Moroccan soil of a Bacillus strain that exhibits an important antimycobacterial activity. The strain was identified as Brevibacillus laterosporus using DNA sequencing of the 16S ribosomal RNA gene. The antimycobacterial activity was assigned to a substance with a protein nature. This nature was revealed using a liquid-liquid extraction with organic solvents, precipitation with ammonium sulfate and treatment with a protease. This study suggested the identification and the characterization of this active metabolite enabling therapeutic investigations further.

Enriching Beneficial Microbial Diversity of Indoor Plants and Their Surrounding Built Environment With Biostimulants

“…The microbiome shift was also visible at genus level; here the proportion of potentially beneficial microorganisms like Methylobacterium, Stenotrophomonas , and Caulobacter increased relatively and significantly for Brevibacillus, Actinoallomurus, Paenibacillus , and Sphaerisporangium . Species of Brevibacillus have potential to act as biological control agents as they were shown to produce chitinases to degrade fungal cell walls (Hassi et al, 2012) or can act pesticidal against insects, nematodes and mollusks (Ruiu, 2013) and plant-associated endophytic Actinobacteria like Actinoallomurus and Sphaerisporangium were already suggested as plant-growth promoting agents in the past (Qin et al, 2011; Hamedi and Mohammadipanah, 2014). Furthermore, the overall loss of microbial diversity was reduced, which took place under our experimental conditions, and the built environment became more plant-like.…”

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“…Similarly, culture filtrate and crude extract of Brevibacillus laterosporus isolated from soil inhibit the growth of Mycobacterium sp. [25]. Penialidin C produced by endophytic fungi Penicillium sp.…”

Medium optimization for production of antifungal active substances from antagonistic Brevibacillus laterosporus and its antifungal activity analysis

“…Brevibacillus spp. can produce a wide variety of metabolites with antifungal activity, which can control plant diseases as biocontrol agents (Sunita et al, 2010;Hassi et al, 2012;Prasanna et al, 2013). A number of these active metabolites are fungicidal or fungistatic peptides that are non-ribosomally synthesized by multienzyme-catalyzed systems (Jing et al, 2012).…”