Activity of disinfectants against foodborne pathogens in suspension and adhered to stainless steel surfaces

Cabeça, Tatiane Karen; Pizzolitto, Antônio Carlos; Pizzolitto, Elisabeth Loshchagin

doi:10.1590/s1517-83822012000300038

Cited by 41 publications

(25 citation statements)

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(1 reference statement)

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“…When sodium hypochlorite is dissolved in cold water, it reacts to form hypochlorous acid, which is a strong oxidizing agent that is effective against foodborne pathogens (S. aureus, L. monocytogenes and E. coli). Hypochlorous acid serves to disinfect surfaces, fruits and vegetables, by killing suspended and film-forming microorganisms [16][17][18][19] . Fruit immersion into sodium hypochlorite solution is usually performed for 15 min, using concentrations between 20 and 100 ppm to reduce the microbial load to permissible levels.…”

Section: Efficiency Is Necessarymentioning

confidence: 99%

Tropical Fruit Pulps: Processing, Product Standardization and Main Control Parameters for Quality Assurance

Silva

Abud

2017

Braz. arch. biol. technol.

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Section: Efficiency Is Necessarymentioning

confidence: 99%

Tropical Fruit Pulps: Processing, Product Standardization and Main Control Parameters for Quality Assurance

Silva

Abud

2017

Braz. arch. biol. technol.

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“…The absence of bacterial cells after sodium hypochlorite treatment in the case of the plate count method could be explained by the fact that bacteria might be dead, injury or none cultivate after treatment. Cabeça et al [35] evaluated five disinfectants against biofilms cells by using plate count method and scanning electron microscopy, and they showed that sodium hypochlorite was the most effective disinfectant against these biofilms compared to others such as biguanide and peracetic acid. While, their results obtained by scanning electron microscopy observations revealed that the bacteria still adhered to stainless steel after all concentrations treatment which is in agreement with our findings.…”

Section: Discussionmentioning

confidence: 99%

“…After 5 min of contact, the surfaces were rinsed 3 times. They were after placed in the glass tubes containing 10 mL of sterile physiological saline, and sonicated at 35 kHz for 10 min and vortexed [4,35]. To quantify viable cells, bacteria were resuspended, serially diluted 10-fold with sterilized physiological saline and cultured on nutrient Agar at the temperature of 37°C for 24 hours [45,47,48].…”

Section: Efficiency Of Sodium Hypochlorite Against Biofilm Cellsmentioning

confidence: 99%

“…These methods include McFarland turbidity test [18], swabbing method [34], microplate absorbance measurement [18], and plate count method. For this latter many techniques are used for biofilm dislodging from a surface such as ultrasonic [34,35], shaking with beads [18] and vortexing [37]. In addition to that, many other techniques are used for biofilm visualization including epifluorescence microscopy [9,[37][38][39], scanning electron microscopy [35,36,40] and atomic force microscopy [41,42].…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of sodium hypochlorite efficiency on the elimination of Pseudomonas aeruginosa biofilm using two methods

Amzil¹,

Hamadi²,

Mimouni³

et al. 2019

Russ Open Med J

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Aims -The purposes of the present work were to study the biofilm formation of Pseudomonas aeruginosa on stainless steel (316 and 304) and to investigate the effectiveness of sodium hypochlorite with different concentrations after 5 min of treatment, against P. aeruginosa biofilm formed on the same substrata. Methods -The methods used in this study were plate count method (PCM) and atomic force microscopy (AFM). Results -The results obtained using PCM showed that bacteria adhered more to stainless steel 316 than stainless steel 304. Furthermore, sodium hypochlorite was effective against cells of P. aeruginosa adhered to stainless steel 304 at the concentration of 0.5 %, but it was not effective against the ones adhered to stainless steel 316 until the concentration of 1%. The AFM results appeared that some bacteria still adhered to two types of stainless steel after sodium hypochlorite treatment at all concentrations. Conclusion -The type of surface and the disinfectant concentration have an effect on the efficiency of the disinfectant against biofilm. In addition the PCM and AFM do not give the same results.

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“…Cabeça et al reported that NaClO was the most effective disinfectant against S. aureus biofilm and biguanide was the least effective, while NaClO and PAA were the most effective disinfectants against Listeria monocytogenes biofilm while biguanide, iodine, and quaternary ammonium composites were the least effective (28).…”

Section: Discussionmentioning

confidence: 99%

The comparison of various disinfectants’ efficacy on Staphylococcus aureus and Pseudomonas aeruginosa biofilm layers

Köse¹,

Yapar²

2017

Turk J Med Sci

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IntroductionA biofilm can be defined as a well-organized microbial community in the extracellular polymeric substance (EPS) that adheres to living or inanimate surfaces (1). Owing to their resistance to antibiotics and antiphagocytic effects, biofilms can frequently cause persistent chronic infections that are difficult to treat. Biofilms are significant causes of morbidity and mortality (2,3). At least 65% of all bacterial infections are associated with biofilm (2,3). Infections such as natural valve endocarditis, otitis media, chronic bacterial prostatitis, cystic fibrosis, and periodontitis develop as a result of biofilms that form on living surfaces. The medical devices on which biofilms can develop include prosthetic heart valves, central venous catheters, urinary catheters, contact lenses, and intrauterine devices (4). Due to the high rates of mortality and morbidity associated with biofilms, several studies have been conducted on antimicrobials and particularly on the effectiveness of antibiotics against biofilms. It has been demonstrated that antibiotics show limited efficacy against biofilms as the biofilm layer persists. For this reason, it should be borne in mind that disinfectants serve as a significant alternative against human mucosa and biofilms on the surfaces of medical device (5). This study aims at investigating the effectiveness of orthophthalaldehyde, peracetic acid, hydrogen peroxide, and sodium hypochlorite against Pseudomonas aeruginosa and Staphylococcus aureus biofilm layers and the live microbial cells on the biofilm layers. Material and methods Bacterial strainsS. aureus (ATCC 6538) and P. aeruginosa (PA01) bacterial strains that are known to produce biofilms were selected (5,6). Bacterial cells stocked at -80 °C were passaged to a tryptic soy agar (TSA) plate (Merck). After being incubated for 24 h at 35-37 °C, a bacterial solution was Background/aim: Because biofilms are resistant to antibiotics and biocides, they usually cause chronic persistent infections, which are arduous to cure and have high mortality and morbidity. Our study aimed to investigate the efficiency of orthophthalaldehyde, peracetic acid, hydrogen peroxide, and sodium hypochlorite on Pseudomonas aeruginosa and Staphylococcus aureus biofilm layers and live microbial cells.Materials and methods: Biofilm layers were determined by crystal violet assay and live microbial cells were determined using a resazurin assay.Results: For Pseudomonas aeruginosa, sodium hypochlorite showed the most influential disinfection because it diminished 83.6% of the biofilm layer and decreased 99.7% of live microbial cells. For Staphylococcus aureus, hydrogen peroxide was determined the most active disinfectant with 80.3% reduction of the biofilm layer. Sodium hypochlorite was also determined to be the most efficient disinfectant with 99.8% reduction of live microbial cells. Sodium hypochlorite was the most influential disinfectant on biofilm layers and live microbial cells of both microorganisms. Conclusion:We concluded that if we use sodi...

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Activity of disinfectants against foodborne pathogens in suspension and adhered to stainless steel surfaces

Cited by 41 publications

References 1 publication

Tropical Fruit Pulps: Processing, Product Standardization and Main Control Parameters for Quality Assurance

Tropical Fruit Pulps: Processing, Product Standardization and Main Control Parameters for Quality Assurance

Evaluation of sodium hypochlorite efficiency on the elimination of Pseudomonas aeruginosa biofilm using two methods

The comparison of various disinfectants’ efficacy on Staphylococcus aureus and Pseudomonas aeruginosa biofilm layers

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