Rapid screening of tannase producing microbes by using natural tannin

Jana, Arijit; Maity, Chiranjit; Halder, Suman Kumar; Pati, Biswamoy; Mondal, Keshab Chandra; Mohapatra, Pradeep Kumar Das

doi:10.1590/s1517-83822012000300034

Cited by 13 publications

(6 citation statements)

References 6 publications

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“…Absorbency was measured at 550 nm after addition of 1 mL of FeCl 3 (0.13 M). One unit of tannase was defined as the amount of enzymerequired to hydrolyze 1μ mole of ester linkage of tannic acid in 1 minute under specific conditions [19].…”

Section: Djulis Fermentationmentioning

confidence: 99%

Enhanced antioxidant activity of Chenopodium formosanum Koidz. by lactic acid bacteria: Optimization of fermentation conditions

et al. 2021

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In this study, different probiotics commonly used to produce fermented dairy products were inoculated independently for Chenopodium formosanum Koidz. fermentation. The strain with the highest level of antioxidant activity was selected and the fermentation process was further optimized via response surface methodology (RSM). Lactobacillus plantarum BCRC 11697 was chosen because, compared to other lactic acid bacteria, it exhibits increased free radical scavenging ability and can produce more phenolic compounds, DPPH (from 72.6% to 93.2%), and ABTS (from 64.2% to 76.9%). Using RSM, we further optimize the fermentation protocol of BCRC 11697 by adjusting the initial fermentation pH, agitation speed, and temperature to reach the highest level of antioxidant activity (73.5% of DPPH and 93.8% of ABTS). The optimal protocol (pH 5.55, 104 rpm, and 24.4°C) resulted in a significant increase in the amount of phenolic compounds as well as the DPPH and ABTS free radical scavenging ability of BCRC 11697 products. The IC50 of the DPPH and ABTS free radical scavenging ability were 0.33 and 2.35 mg/mL, respectively, and both protease and tannase activity increased after RSM. An increase in lower molecular weight (<24 kDa) protein hydrolysates was also observed. Results indicated that djulis fermented by L. plantarum can be a powerful source of natural antioxidants for preventing free radical-initiated diseases.

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Section: Djulis Fermentationmentioning

confidence: 99%

Enhanced antioxidant activity of Chenopodium formosanum Koidz. by lactic acid bacteria: Optimization of fermentation conditions

et al. 2021

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“…Ellagitannase producers were screened with the help of methyl red (0.01 M methyl red in 0.01 N HCl) as pH indicator and flooded on the plates were kept for 5-10 min. 17 Ellagitannase producers that showed red hallow zone around the colony were recorded as positive.…”

Section: Isolation and Screening Of Ellagitannase Producermentioning

confidence: 99%

Production and perspective approach of ellagic acid from mango pulp industrial waste by the Micrococcus luteus

Anandan

Kannan

Sudalaimani

et al. 2023

Env Prog and Sustain Energy

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Ellagic acid (EA) has gained momentum recently due to its various properties like anti‐mutagenic, anti‐carcinogenic, anti‐oxidant, anti‐viral and many other benefits to human health. The present study focused on the microbial production of EA from mango pulp processing industrial waste as an alternate method for conventional chemical extraction. Our experiments demonstrated that the 100 μg/mL of EA was produced by Micrococcus luteus from 9% of mango pulp waste and the effect of several fermentation parameters of EA production with Pontecorvo medium has yielded 496.75 mg/g at pH 6.0, temperature 35°C, ammonium nitrate (nitrogen source), sucrose (carbon source), potassium chloride (mineral source) with 0.5% of inoculum after 48 h of incubation. EA synthesized was further confirmed with the standard EA in high performance liquid chromatography and Fourier transform infrared spectroscopy. Perspective approaches like drought resistant in plants, anti‐parasitic activity and anti‐cancer activities have been proven and they were docked against depression proteins (receptors), namely MAO‐A using Epic, LigPrep and Glide module of Schrödinger. EA was found to have an ability to bind with the candidate receptor proteins 2Z5X for depression. Microbial bioconversion of mango wastes from pulp processing industries which can reduce the environmental pollution and convert as value added product.

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“…The microbial mat growing on the oneyear-old aqueous areca extract was used as a source of inoculum. The sample collected was soaked in sterile distilled water and was spread on potato dextrose agar (PDA) and incubated at 30°C for 72 h 17,18 . The isolated pure cultures were further stored at 4°C.…”

Section: Isolation and Identificationmentioning

confidence: 99%

Production, Purification and Characterization of Extracellular Tannase from a Newly Isolated Yeast, Geotrichum cucujoidarum

Thangavelu¹,

Hugar²,

Belur³

2022

J. Pure Appl. Microbiol.

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With an aim to isolate a tannase positive organism, the microbial mat growing on the stored areca extract leachate surface was screened. Once the tannase positive organism was isolated, it was identified by ITS/18S rRNA gene sequencing. Further, the enzyme was purified and examined for its biochemical properties. A potent extracellular tannase-producing yeast was isolated and was identified as Geotrichum cucujoidarum. After the shake flask studies, the enzyme activity of 4.42 U/ml and specific activity of 29.86 U/mg were achieved in a medium with tannic acid as an inducer. Later, ethanol (70%) precipitation followed by purification through FPLC using SEC 650 column resulted in 166.37 U/mg specific activity and a recovery of 50.54%. The purified enzyme was a monomer with a molecular weight of 63 kDa. The optimum pH and the temperature of the enzyme were found to be 5.0 and 30°C, respectively. The Michaelis-Menten constant (Km) was found to be 2.9 mM, and the turn over number (kcat) and catalytic efficiency (kcat/km) of the purified tannase were 102 S-1 and 35.17 mM-1S-1 respectively. Temperature and pH stability profiles of the enzyme, influence of various metal ions, chelators and surfactants on enzyme activity and kinetic constants of enzyme shows that the tannase produced from Geotrichum cucujoidarum is unique and is a potential candidate for further studies.

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Rapid screening of tannase producing microbes by using natural tannin

Cited by 13 publications

References 6 publications

Enhanced antioxidant activity of Chenopodium formosanum Koidz. by lactic acid bacteria: Optimization of fermentation conditions

Enhanced antioxidant activity of Chenopodium formosanum Koidz. by lactic acid bacteria: Optimization of fermentation conditions

Production and perspective approach of ellagic acid from mango pulp industrial waste by the Micrococcus luteus

Production, Purification and Characterization of Extracellular Tannase from a Newly Isolated Yeast, Geotrichum cucujoidarum

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