Detection of Genetic characterization of Porcine circovirus 2 (PCV2) in Brazilian wildlife boars

Castro, Alessandra Marnie Martins Gomes de; Castro, F.G.; Budiño, F.E.L.; Baldin, C.M.; Silva, S.O.S.; Brandão, P.E.; Richtzenhain, Leonardo José

doi:10.1590/s1517-83822012000300025

Cited by 13 publications

(9 citation statements)

References 5 publications

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“…In fact, despite PCV2 being identi ed as a swine pathogen in the late 90s, retrospective studies and analysis based on the molecular clock revealed a much more ancient origin and undetected circulation [6]. Similarly, di erent studies have revealed PCV2 infection in wild boars and other wild animals, although negligible evidence of clinical signs has been provided in these species [7][8][9][10][11][12][13]. Also, extensively raised pigs appeared less a ected.…”

Section: Introductionmentioning

confidence: 99%

Molecular Epidemiology and Genetic Characterization of PCV2 Circulating in Wild Boars in Southwestern Ethiopia

Tegegne

Tsegaye

Aman

et al. 2022

Journal of Tropical Medicine

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Porcine circovirus type 2 (PCV2) is one of the most relevant infectious agents affecting domestic pigs. Recently, a surprising PCV2 genetic heterogenicity has been reported in Africa. Nevertheless, the knowledge of the epidemiology of PCV2 in African countries, in both domestic and wild species, is limited and sparse. Having this in mind, in the present study, the PCV2 circulation and its molecular epidemiology in Southwestern Ethiopia have been investigated by collecting 64 samples from domestic pigs, wild boars, and warthogs. PCV2 genome presence was detected and quantified using qPCR and ORF2 sequencing was attempted on positive samples. Ten samples, 8 wild boars, 1 domestic pig, and 1 warthog, tested PCV2 positive. Complete ORF2 sequences were obtained from 5 wild boars; 4 of those were classified as PCV2d and 1 as PCV2b. Both PCV2b and PCV2d were related to strains of Asian origin, most commonly from China. The role of this country in the exportation of PCV2 strains in Ethiopia, and Africa in general, might be supported by the crescent economic relationship between the two continents. The obtained evidence also testifies to the inadequacy and/or poor application of biosecurity measures separating wild and domestic animals. Further, extensive and systematic studies should be performed to more deeply characterize the molecular epidemiology of PCV2 in this region, in order to improve our understanding of these ecological niches in the evolution and dispersal of PCV2.

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Section: Introductionmentioning

confidence: 99%

Molecular Epidemiology and Genetic Characterization of PCV2 Circulating in Wild Boars in Southwestern Ethiopia

Tegegne

Tsegaye

Aman

et al. 2022

Journal of Tropical Medicine

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“…PDCoV was first discovered in Hong Kong, China, in 2012 in a territory-wide molecular epidemiology study in mammals and birds (Woo et al, 2012). PDCoV was subsequently reported in the United States in 2014 (Castro et al, 2012;Wang et al, 2014) and then in South Korea, mainland China, Japan, Thailand, Viet Nam and Lao People's Democratic Republic (Lao PDR) (Lee and Lee, 2014;Lorsirigool et al, 2016;Saeng-Chuto et al, 2017a;Song et al, 2015). The coinfection of PDCoV with other enteric viral pathogens such as PEDV, rotavirus or kobuvirus are commonly reported (Mai et al, 2018;Marthaler et al, 2014a;Marthaler et al, 2014b).…”

Section: Introductionmentioning

confidence: 99%

Detection and spike gene characterization in porcine deltacoronavirus in China during 2016–2018

Cheng

Xing

et al. 2019

Infection, Genetics and Evolution

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A B S T R A C TPorcine deltacoronavirus (PDCoV) has been emerging in several swine-producing countries for years. In our study, 719 porcine diarrhoea samples from 18 provinces in China were collected for PDCoV and porcine epidemic diarrhoea virus (PEDV) detection. The epidemiological survey revealed that the positive rates of PDCoV, PEDV and coinfection were 13.07%, 36.72% and 4.73%, respectively. The entire spike (S) genes of eleven detected PDCoV strains were sequenced. Phylogenetic analysis showed that the majority of PDCoVs could be divided into three lineages: the China lineage, the USA/Japan/South Korea lineage and the Viet Nam/Laos/ Thailand lineage. The China and the Viet Nam/Laos/Thailand lineages showed much greater genetic divergences than the USA/Japan/South Korea lineage. The present study detected one new monophyletic branch that contained three PDCoVs from China, and this branch was separated from the China lineage but closely related to the Viet Nam/Laos/Thailand lineage. The strain CH-HA2-2017, which belongs to this new branch, had a possible recombination event between positions 27 and 1234. Significant amino acid substitutions of PDCoV S proteins were analysed and displayed with a three-dimensional cartoon diagram. The visual spatial location of these substitutions gave a conformational-based reference for further studies on the significance of critical sites on the PDCoV S protein.

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“…The detection of the PCV2 ORF1 and ORF2 genes, and PCV3 ORF1 gene were previously reported (Castro et al 2012;. The PCV2 strain Stoon 1010 obtained from The Scientific Veterinary Institute "Novi Sad" from Novi Sad, Serbia, and an internal reference PCV3 strain from the Department of Microbiology of the Faculty of Veterinary Medicine, University of Belgrade served as the controls.…”

Section: Pcv2 and Pcv3 Detectionmentioning

confidence: 91%

Detection and characterisation of porcine circoviruses in wild boars in northeastern Serbia

Nišavić¹,

Milic²,

Radalj³

et al. 2022

Vet. Med.

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The objective was to expand and update the knowledge on the presence and genotype diversity of porcine circoviruses 2 and 3 (PCV2 and PCV3) in the wild boar populations from the hunting grounds in northeastern Serbia. The presence of PCV3 was not determined, and PCV2 was confirmed in 40.32% of the organ samples from 124 wild boars hunted from 2018 to 2019, indicating their significance in virus circulation since traditional pig farms with irregular PCV2 vaccination strategies are widespread in this region. The most prevalent genotype was PCV2d, followed by PCV2b and PCV2a in 55.6%, 38.9%, and 5.5% of the examined samples, respectively. Nucleotide sequences of the detected strains were homogenous within the genotype and clustered within the subgroups PCV2d-2, PCV2b-1A/B, and PCV2a-2D with high identity to European, Chinese, and Serbian domestic pig sequences suggesting their origin. Wild boars presented with no clinical or pathological signs of infection, implying that these animals might be less susceptible to disease, particularly since the cofactors present in pig farming systems that support the disease development are absent in the wild. The high PCV2 detection frequency demonstrates the importance of wildlife monitoring to track virus population dynamics, especially in regions with free-range pig farming in order to plan adequate disease control strategies.

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Detection of Genetic characterization of Porcine circovirus 2 (PCV2) in Brazilian wildlife boars

Cited by 13 publications

References 5 publications

Molecular Epidemiology and Genetic Characterization of PCV2 Circulating in Wild Boars in Southwestern Ethiopia

Molecular Epidemiology and Genetic Characterization of PCV2 Circulating in Wild Boars in Southwestern Ethiopia

Detection and spike gene characterization in porcine deltacoronavirus in China during 2016–2018

Detection and characterisation of porcine circoviruses in wild boars in northeastern Serbia

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