Isolation of the first IMP-4 metallo-&amp;#946;-lactamase producing Klebsiella pneumoniae in Tianjin, China

Li, Jing; Hu, Zhidong; Hu, Qiaojuan

doi:10.1590/s1517-83822012000300010

Cited by 10 publications

(3 citation statements)

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“…PCR was carried out in 25 μL reactions using 12.5 μL of PCR master mix (Nippon genetics, Europe), 1 μL (12.5 pmol) of each primer, 2μL (20ng) of template DNA and 8.5 μL of sterile water. The amplification reactions were carried out by conventional PCR using Biometra, UNO II thermal cycler under the following conditions: for blaIMP-1, blaIMP-2 and blaVIM-1; initial denaturation at 94°C for 5 minutes, followed by 35 cycles each consists of denaturation at 94°C for 30 seconds, annealing at 55°C for 30 seconds, extension at 72 °C for 90 seconds with a final extension step at 72°C for 10 minutes; for blaVIM-2 gene the same conditions were used except that the initial denaturation was for 3 minutes [12,13]. The resulting products were loaded in 1.5% agarose gel and were visualized under UV illumination system.…”

Section: Dna Extraction and Pcrmentioning

confidence: 99%

High incidence of MBL-mediated imipenem resistance among Pseudomonas aeruginosa from surgical site infections in Egypt

Raouf

Sayed

Rizk

et al. 2018

J Infect Dev Ctries

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Introduction: Surgical wound infection is a serious problem, especially with metallo-beta lactamases (MBLs)-producing gram-negative bacteria as Pseudomonas aeruginosa. The main objective of this work was to evaluate for the first time in Minia-Upper Egypt, the incidence of imipenem-resistant Pseudomonas aeruginosa infection of surgical wounds particularly that mediated by MBL production. Methodology: P. aeruginosa was isolated from infected wounds by swabs and underwent full microbiological identification and Antibiotic Susceptibility testing. MBL production was tested by E-test and PCR was used for imipenemase (blaIMP) and Verona integron-encoded metallobeta-lactamase (blaVIM) gene detection. Results: Out of 200 pus samples collected from surgical site infections, P. aeruginosa had the prevalence rate of 35%. Imipenem resistance was found in 28.57% of the isolated Pseudomonas aeruginosa. The prevalence of MBL-producing isolates among Imipenem-resistant P. aeruginosa (IRPA) was 85 % by phenotypic method with 29% of them harboring blaVIM gene. High resistance rates to other classes of antibiotics were reported among the isolates with multi-drug resistance (MDR) detected in 97.3% of the isolates. Conclusion: To our knowledge, this is the first report in Minia, Upper Egypt describing the relatively high incidence of IRPA in infected surgical wounds with MBLs involved in the majority of isolates.

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Section: Dna Extraction and Pcrmentioning

confidence: 99%

High incidence of MBL-mediated imipenem resistance among Pseudomonas aeruginosa from surgical site infections in Egypt

Raouf

Sayed

Rizk

et al. 2018

J Infect Dev Ctries

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“…MBL-producing K. pneumoniae isolates have usually been reported sporadically in China (Mendes et al 2008;Liu et al 2009;Li et al 2012;Wang et al 2014), and some researches have demonstrated, at most, a low-level endemicity of MBL producers in hospitals where similar strains have been detected (Chen et al 2011;Liu et al 2014). Here, we describe the emergence of high-level endemicity for MBL-producing K. pneumoniae, which has recently occurred in a teaching hospital of southeastern China.…”

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confidence: 99%

High prevalence of metallo-β-lactamase among carbapenem-resistant Klebsiella pneumoniae in a teaching hospital in China

Zhao

et al. 2014

Can. J. Microbiol.

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The aim of this study was to characterize the carbapenemases in carbapenem-resistant Klebsiella pneumoniae (CR-KP) from a Chinese teaching hospital. A total of 40 CR-KPs were screened for the presence of carbapenemases. Minimum inhibitory concentrations were determined by agar dilution. The modified Hodge test was used for the detection of carbapenemase production. Carbapenemase, extended-spectrum β-lactamase, and AmpC genes were detected using polymerase chain reaction (PCR) and sequencing. A conjugation test was performed using a broth culture mating method, transferred plasmids were typed by PCR-based replicon typing, and clonal relatedness was investigated by enterobacterial repetitive intergenic consensus sequences PCR (ERIC-PCR) and multilocus sequence typing (MLST). The results revealed that modified Hodge test was positive for 28 CR-KPs, and CR-KPs exhibited high resistance rates against various antibiotics, except colistin (5.0%) and tigecycline (22.5%). ERIC and MLST profiles showed no clonal outbreak. PCR demonstrated a high prevalence rate (55.0%, 22/40) of metallo-β-lactamases (MBLs) in CR-KPs. IMP-4, IMP-8, NDM-1, and KPC-2 were identified in 14 (35.0%), 7 (17.5%), 2 (5.0%), and 7 (17.5%) isolates, respectively. Notably, 2 CR-KPs coproduced 2 carbapenemases simultaneously (IMP-8/NDM-1 and IMP-4/KPC-2). In vitro transfer of carbapenem resistance was successful for 11 MBL-producing CR-KPs. The extended spectrum β-lactamase genes were detected in 30 (75.0%) of these CR-KPs. To the best of our knowledge, this is the first report focusing on carbapenem resistance in K. pneumoniae due to metalloenzymes in China. Screening and surveillance of MBLs in Enterobacteriaceae is urgently needed in this region to control and prevent the spread of these resistance determinants.

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“…9,10 Plasmid-borne bla IMP-4 has been sporadically reported in different Gram-negative bacilli in China. 6,[11][12][13] However, only a few studies have reported the complete sequence of bla IMP-4 -harbouring plasmids, 6,10,14 which limits our understanding of the transmission mechanism of bla IMP-4 between different Gram-negative bacilli.…”

Section: Introductionmentioning

confidence: 99%

<p>Characterization of an IMP-4-Producing <em>Klebsiella pneumoniae</em> ST1873 Strain Recovered from an Infant with a Bloodstream Infection in China</p>

Lin

Chen

et al. 2020

IDR

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Imipenemase (IMP), an Ambler class B metallo-β-lactamase, is an important carbapenemase that confers resistance to almost all β-lactams. In this study, we characterized the genomic feature of an IMP-4-producing Klebsiella pneumoniae ST1873 strain, a rare sequence type (ST) isolated from an infant with a bloodstream infection in China. Patients and Methods: K. pneumoniae strain, BKP19, was collected from a bloodstream infection in an infant who was hospitalized at the department of paediatrics. The whole genome sequence of the strain was sequenced using the Illumina NovaSeq 6000 platform and long-read MinION sequencer. Multilocus sequence typing, antimicrobial resistance gene identification, plasmid and phylogenetic relationship analysis of the strain were analysed by various bioinformatics approaches. Results: K. pneumoniae BKP19 was resistant to multiple antimicrobials, including carbapenems. Eleven antimicrobial resistance genes corresponding to beta-lactam resistance, quinolone resistance, phenicol resistance and fosfomycin resistance could be identified in the genome. The carbapenem resistance gene bla IMP-4 was located in an IS26-associated class 1 integron of an IncN-type plasmid with 39,033 bp (pIMP-4-BKP19). Sequence alignment revealed that pIMP-4-BKP19 is closely related to the common plasmid carrying IMP-4 in K. pneumoniae (pIMP-HZ1-like plasmid) but is smaller, lacking the quinolone resistance gene qnrS1 and multiple tra gene orthologs. Conjugation experiment revealed that pIMP-4-BKP19 is a non-conjugative plasmid. According to in silico MLST analysis, K. pneumoniae strain BKP19 belongs to a sporadic clone ST1873. Conclusion: In summary, our study reports the first genome sequence of a K. pneumoniae ST1873 strain harbouring the class B β-lactamase bla IMP-4 in an IncN-type plasmid recovered from an infant with a bloodstream infection in China. Considering the global emergence of IMP-4 carbapenemase, more attention must be paid to prevent its future prevalence.

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Isolation of the first IMP-4 metallo-β-lactamase producing Klebsiella pneumoniae in Tianjin, China

Cited by 10 publications

References 0 publications

High incidence of MBL-mediated imipenem resistance among Pseudomonas aeruginosa from surgical site infections in Egypt

High incidence of MBL-mediated imipenem resistance among Pseudomonas aeruginosa from surgical site infections in Egypt

High prevalence of metallo-β-lactamase among carbapenem-resistant Klebsiella pneumoniae in a teaching hospital in China

<p>Characterization of an IMP-4-Producing <em>Klebsiella pneumoniae</em> ST1873 Strain Recovered from an Infant with a Bloodstream Infection in China</p>

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