Biodegradation of petroleum hydrocarbons in hypersaline environments

Martins, Luiz Fernando; Peixoto, Raquel S.

doi:10.1590/s1517-83822012000300003

Cited by 63 publications

(31 citation statements)

References 47 publications

(77 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among bacteria, members of the genera Halomonas, Marinobacter, and Alcanivorax have been widely reported to degrade aliphatic and aromatic compounds over a broad range of salinities (1,(6)(7)(8)(9). The majority of published reports indicate that Halomonas spp.…”

mentioning

confidence: 99%

Arhodomonas sp. Strain Seminole and Its Genetic Potential To Degrade Aromatic Compounds under High-Salinity Conditions

Dalvi

Nicholson

Najar

et al. 2014

Appl Environ Microbiol

View full text Add to dashboard Cite

cArhodomonas sp. strain Seminole was isolated from a crude oil-impacted brine soil and shown to degrade benzene, toluene, phenol, 4-hydroxybenzoic acid (4-HBA), protocatechuic acid (PCA), and phenylacetic acid (PAA) as the sole sources of carbon at high salinity. Seminole is a member of the genus Arhodomonas in the class Gammaproteobacteria, sharing 96% 16S rRNA gene sequence similarity with Arhodomonas aquaeolei HA-1. Analysis of the genome predicted a number of catabolic genes for the metabolism of benzene, toluene, 4-HBA, and PAA. The predicted pathways were corroborated by identification of enzymes present in the cytosolic proteomes of cells grown on aromatic compounds using liquid chromatography-mass spectrometry. Genome analysis predicted a cluster of 19 genes necessary for the breakdown of benzene or toluene to acetyl coenzyme A (acetyl-CoA) and pyruvate. Of these, 12 enzymes were identified in the proteome of toluene-grown cells compared to lactate-grown cells. Genomic analysis predicted 11 genes required for 4-HBA degradation to form the tricarboxylic acid (TCA) cycle intermediates. Of these, proteomic analysis of 4-HBA-grown cells identified 6 key enzymes involved in the 4-HBA degradation pathway. Similarly, 15 genes needed for the degradation of PAA to the TCA cycle intermediates were predicted. Of these, 9 enzymes of the PAA degradation pathway were identified only in PAA-grown cells and not in lactate-grown cells. Overall, we were able to reconstruct catabolic steps for the breakdown of a variety of aromatic compounds in an extreme halophile, strain Seminole. Such knowledge is important for understanding the role of Arhodomonas spp. in the natural attenuation of hydrocarbon-impacted hypersaline environments.

show abstract

mentioning

confidence: 99%

Arhodomonas sp. Strain Seminole and Its Genetic Potential To Degrade Aromatic Compounds under High-Salinity Conditions

Dalvi

Nicholson

Najar

et al. 2014

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…(Abed et al 2006;Cohen 2002). Similar studies concerning degradation of hydrocarbon pollutants by aerobic cyanoheterotrophic bacteria (Abed and Köster (2005) and in various aquatic environments have also been described (Leahy and Colwell 1990;Martins and Peixoto 2012). The complexity of this population suggests that bioremediation involves metabolic complementary functions because oxygenic photosynthesis of these oil-insensitive cyanobacteria symbiotically supply molecular oxygen for efficient aerobic metabolism (Abed et al 2014).…”

Section: Degradation Of Petroleum Hydrocarbons By Microbial Communitiesmentioning

confidence: 76%

“…However, reviews concerning petroleum hydrocarbon degradation in the presence of substantial salt concentrations are limited in number (Fathepure 2014;Le Borgne et al 2008;Martins and Peixoto 2012;McGenity 2010;Patzelt 2005). Hydrocarbon biodegradation in a hypersaline or marine environment is important as it concerns the bioremediation of petroleum pollution of salt marshes and industrial wastewaters.…”

Section: Petroleummentioning

confidence: 99%

Halophiles: biology, adaptation, and their role in decontamination of hypersaline environments

Edbeib

Wahab

Huyop

2016

World J Microbiol Biotechnol

120

View full text Add to dashboard Cite

The unique cellular enzymatic machinery of halophilic microbes allows them to thrive in extreme saline environments. That these microorganisms can prosper in hypersaline environments has been correlated with the elevated acidic amino acid content in their proteins, which increase the negative protein surface potential. Because these microorganisms effectively use hydrocarbons as their sole carbon and energy sources, they may prove to be valuable bioremediation agents for the treatment of saline effluents and hypersaline waters contaminated with toxic compounds that are resistant to degradation. This review highlights the various strategies adopted by halophiles to compensate for their saline surroundings and includes descriptions of recent studies that have used these microorganisms for bioremediation of environments contaminated by petroleum hydrocarbons. The known halotolerant dehalogenase-producing microbes, their dehalogenation mechanisms, and how their proteins are stabilized is also reviewed. In view of their robustness in saline environments, efforts to document their full potential regarding remediation of contaminated hypersaline ecosystems merits further exploration.

show abstract

“…Studies of [26] and [27] it is clear that very low salt concentrations reduce hydrocarbonoclastic activity and the optimum biodegradation results are reached whitin moderate salinity ranges. First of all, it is necessary to consider that hydrocarbons are less bioavailable in hypersaline environments that in nonsaline ones [28].…”

Section: Resultsmentioning

confidence: 99%

Efficiency of pseudomonas aeruginosa and Escherichia coli to treatment of effluent petroleum wastewater from AI-Nasiriya refinery – Iraq

AI-Jawhari

Mhail

AI-RuthaAli

2015

IJBAS

View full text Add to dashboard Cite

Fourbacteria isolated from the soil contaminated with petroleum hydrocarbon and wastewaters from the refinerylocated in the south part of AI-Nasiriya city in Iraq,by using dilution method.The results showed that Pseudomonas aeruginosawas more dominant with 100% in soil and wastewaters in inside of refinery,and 93%,95% in soil and wastewaters in outside the refinery respectively.The results showed that the second dominant of bacteria was Esherchia coil with 92%,98% in soil and wastewaters in inside the refinery,and 83%,88% in soil and wastewaters in outside of refinery.The remaining two species of bacteria isolated with a very low frequency,these bacteria were Bacillus subtilis and Klebsiella sp., B.subtilis was obtained with 10%,8% in soil and wastewaters in inside the refinery,and 10%,3% in soil and wastewaters in outside of refinery .In the same time that Klebsiella sp. Was obtained with 10% in soil and wastewaters in inside the refinery,and 8%,3% in soil and wastewaters in outside the refinery.The results showed that mixed two bacteria (high frequency)were more efficient to decrease Biological Oxygen Demande(BOD)with 59% in pH 4,0.5gm / I salt (NaCI) concentration.Chemical Oxygen Demande (COD) was decreased to 75%.The results showed that the Total Acidity(TA) in wastewater were increased to 82%mg / I in 40c o ,pH 8 and 2.0 gm/ I NaCI , and Total Suspended Solid (TSS) was decreased to 50 mg / I in 40c o , pH 8,2.0 gm / I NaCI . The results showed that the Total Petroleum Hydrocarbon (TPH) was removal with 99.99%, and in the same time the numbers of mixed bacteria were increased to 5x10 8 after incubation 48h in 40c o .

show abstract

Biodegradation of petroleum hydrocarbons in hypersaline environments

Cited by 63 publications

References 47 publications

Arhodomonas sp. Strain Seminole and Its Genetic Potential To Degrade Aromatic Compounds under High-Salinity Conditions

Arhodomonas sp. Strain Seminole and Its Genetic Potential To Degrade Aromatic Compounds under High-Salinity Conditions

Halophiles: biology, adaptation, and their role in decontamination of hypersaline environments

Efficiency of pseudomonas aeruginosa and Escherichia coli to treatment of effluent petroleum wastewater from AI-Nasiriya refinery – Iraq

Contact Info

Product

Resources

About