Diagnosis of paratuberculosis in cattle: microbiological culture, serology and PCR

Carvalho, Isabel Azevedo; Campos, Vinicius E.B.; Souza, Iana M; Zanardo, Larissa G.; Filho, José Dantas Ribeiro; Gomes, Marcos José Pereira; Moreira, Maria Aparecida Scatamburlo

doi:10.1590/s1517-83822012000200020

Cited by 7 publications

(7 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Pasteurized milk: Reported bio-load of MAP by different workers using milk culture was 2. 8, 1.8-6.7, 2.7, 2.8, 15.0, 6.9, 1.6% in USA, UK, Brazil, Argentina, Ontario, N. Ireland and Czech republic, respectively (Millar et al 1996;Grant et al 2002a;Stabel 2002;O'Reilly et al 2004;Ayele et al 2005;Ellingson et al 2005;Grant 2005;Carvalho et al 2012aCarvalho et al , 2012bPaolicchi et al 2012).…”

Section: Map In Dairy Productsmentioning

confidence: 99%

Mycobacterium aviumsubspeciesparatuberculosis –an important food borne pathogen of high public health significance with special reference to India: an update

Chaubey

Singh

Gupta

et al. 2017

Veterinary Quarterly

View full text Add to dashboard Cite

This review underlines the public health significance of 'Indian Bison Type' of Mycobacterium avium subspecies paratuberculosis (MAP) and also its potential as 'zoonotic infection'. In the absence of control programs, bio-load of MAP is increasing and if we take total population of animals (500 million plus) and human beings (1.23 billion plus) into account, the number of infected animals and human beings will run into millions in India. Our research on screening of over 26,000 domestic livestock for MAP infection using 4 different diagnostic tests (microscopy, culture, ELISA and PCR), during last 31 years has shown that the average bio-load of MAP in the livestock population of India is very high (cattle 43%, buffaloes 36%, goats 23% and sheep 41%). 'Mass screening' of 28,291 human samples between 2008-2016 revealed also high bio-load of MAP. It has been proved that MAP is not in-activated during pasteurization and therefore live bacilli are continuously reaching human population by consumption of even pasteurized milk and other milk products. Live bacilli have also been recovered from meat products and the environment thus illustrating the potential of MAP as pathogen of public health concern. However, at present, there is inadequate scientific evidence to confirm a conclusive link between MAP infection and Johne's disease in ruminants and some cases of Crohn's disease in human beings.

show abstract

Section: Map In Dairy Productsmentioning

confidence: 99%

Mycobacterium aviumsubspeciesparatuberculosis –an important food borne pathogen of high public health significance with special reference to India: an update

Chaubey

Singh

Gupta

et al. 2017

Veterinary Quarterly

View full text Add to dashboard Cite

show abstract

“…; Carvalho et al . ; Paolicchi et al . ) suggesting that the pathogen can enter the human food chain and that current high temperature, short‐time pasteurization may not always ensure complete inactivation of this pathogenic bacterium.…”

Section: Introductionmentioning

confidence: 99%

“…Consumption of milk, and possibly meat, from infected animals is currently viewed as a potential source of zoonotic transmission of the pathogen from animals to humans. MAP has been isolated from retail milk and dairy products in many parts of the world (Grant et al 2002;Ayele et al 2005;Ellingson et al 2005;Carvalho et al 2012;Paolicchi et al 2012) suggesting that the pathogen can enter the human food chain and that current high temperature, short-time pasteurization may not always ensure complete inactivation of this pathogenic bacterium.…”

Section: Introductionmentioning

confidence: 99%

Sensitive and specific detection of viableMycobacterium aviumsubsp.paratuberculosisin raw milk by the peptide-mediated magnetic separation-phage assay

Foddai

Grant

2017

J Appl Microbiol

View full text Add to dashboard Cite

show abstract

“…El diagnóstico de la PTB en rumiantes incluye varios tipos de pruebas. La sensibilidad (Se) y la especificidad (Sp) de las pruebas de diagnóstico varían según el estadio clínico de la infección (2). Debido a la rusticidad de los búfalos, es probable que la PTB se produzca de forma asintomática, lo que lleva a una diseminación silenciosa de la bacteria, no solo dentro del hato, sino a otras poblaciones de rumiantes (3,4).…”

Section: Introductionunclassified

Mycobacterium avium subsp. paratuberculosis (MAP) en un hato lechero de búfalos en Colombia

et al. 2021

View full text Add to dashboard Cite

Objetivo. Determinar la presencia de Mycobacterium avium subsp. paratuberculosis (MAP) mediante métodos serológicos, microbiológicos y moleculares, para genotipar los aislamientos de MAP y explorar factores asociados a la seropositividad a MAP en un hato de búfalos del departamento de Antioquia (Colombia). Materiales y métodos. Se realizó un estudio transversal para analizar muestras fecales y de suero de 21 búfalas adultas asintomáticas mediante cultivo y ELISA, respectivamente. Los cultivos aislados sospechosos se confirmaron mediante PCR de MAP-IS900 (IS900-PCR). El ADN positivo se volvió a analizar mediante PCR cuantitativo de MAP-IS900 (IS900-qPCR), y luego, se subtipificó utilizando unidades repetitivas intercaladas de micobacterias-número variable de repeticiones en tándem (MIRU-VNTR) y repetición de secuencia corta de múltiples locus (MLSSR). Resultados. Al muestreo, la edad promedio de los animales era de 4.7 años, el 90.5% (19/21) eran hembras y el 84.2% (16/19) estaban preñadas. El puntaje promedio de condición corporal fue 4.2 (escala de 1 a 5). La producción de leche promedio fue de 3.5 L/día/animal. No se detectaron animales seropositivos a MAP. Una hembra de los 21 búfalos fue positiva mediante cultivo fecal en la semana seis de incubación y luego se confirmó mediante IS900-PCR. El análisis de subtipado reveló que el aislado de MAP correspondía al genotipo INMV 3 por MIRU-VNTR. Conclusiones. Según el conocimiento de los autores, este es el primer diagnóstico y aislamiento de MAP en búfalos en Colombia y la primera vez que se encuentra un perfil INMV 3 de MAP en esta especie en el país.

show abstract

Diagnosis of paratuberculosis in cattle: microbiological culture, serology and PCR

Cited by 7 publications

References 18 publications

Mycobacterium aviumsubspeciesparatuberculosis –an important food borne pathogen of high public health significance with special reference to India: an update

Mycobacterium aviumsubspeciesparatuberculosis –an important food borne pathogen of high public health significance with special reference to India: an update

Sensitive and specific detection of viableMycobacterium aviumsubsp.paratuberculosisin raw milk by the peptide-mediated magnetic separation-phage assay

Mycobacterium avium subsp. paratuberculosis (MAP) en un hato lechero de búfalos en Colombia

Contact Info

Product

Resources

About