Characterization and lytic activity of Pseudomonas fluorescens phages from sewage

Radhakrishnan, Ananthi; Ananthasubramanian, M

doi:10.1590/s1517-83822012000100042

Cited by 3 publications

(2 citation statements)

References 17 publications

(17 reference statements)

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“…Similarly, Nocardiophages were recently isolated from wastewater for control of Nocardia species that pose a foaming problem in activated sludge process (ASP), worldwide 28 . Likewise, other reports have also shown phage application in pathogen reduction 17,39,[44][45][46] . Phage application as therapeutic agent to combat bacterial infection needs to be further explored along with in vivo models and clinical trials.…”

Section: Discussionmentioning

confidence: 83%

Isolation and Characterization of Lytic Bacteriophages from Wastewater and its Application in Pathogen Reduction

et al. 2020

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Present work wasperformedto isolate, enrich and characterizebacteriophageusing basic laboratory set-up, and to demonstrate their lytic potential in pathogen reduction. Following standard microbiological and molecular biological procedures, we examined cultivable bacteria and bacteriophages in wastewater sample. Subsequently, from cultures of wastewater sample, we isolated and characterized bacterial isolate, which was thenusedtoenrichlytic bacteriophages, using a combination of double layer plaque-assay, isolation and genetic techniques to isolate bacteriophage against Pseudomonas aeruginosa. Subsequently decontamination capability of this phage was assessed in form of spray and matrix adsorbed formulations. Storage stability of phagepreparations at different temperatures was also studied by calculating reduction in phage titer. Presentstudies demonstratelytic potential of phageas an eco-friendly alternative to conventional control approachesagainstbacterial contamination.

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Section: Discussionmentioning

confidence: 83%

Isolation and Characterization of Lytic Bacteriophages from Wastewater and its Application in Pathogen Reduction

et al. 2020

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“…The authors of another study (Radhakrishnan and Ananthasubramanian 2012 ) conducted on phages specific for Pseudomonas fluorescens , suggest that the molecular weights of the major proteins do not vary among phage lysates specific to the same bacterial strains.…”

Section: Discussionmentioning

confidence: 99%

Protein profiles of bacteriophages of the family Myoviridae-like induced on M. haemolytica

et al. 2018

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The aim of study was to isolate, characterize and analyse the protein profiles of Myoviridae-like bacteriophages obtained from M. haemolytica using MALDI TOF mass spectrometry. The material consisted of the M. haemolytica reference strain ATCC® BAA410, reference serotypes A1, A2, A5, A6, A7, A9, and A11, and wild-type isolates of serotype A1. Bacteriophage morphology was examined with a transmission electron microscope. The proteins were separated in SDS-PAGE and two-dimensional electrophoresis and characterized by MALDI-TOF. Among the phages obtained, seven were specific for strains A1, A2, A5, A6, A7 and 25, and PHL-1 was specific for the BAA410 strain. The protein profiles for the phages were very similar to one another, but differed from the reference phage in that they lacked protein fractions with molecular weights of 22.9, 56.3 and 73.1 kDa. 2D electrophoresis revealed significant differences in the size of proteins and their localization in the pH gradient. The most similar profiles were observed in phages specific for strains BAA-410 and A6. In all profiles two main spots were observed in the molecular weight range from 44 to 70 kDa at pH < 4. The results indicate that 2D electrophoresis is a very useful tool for characterization of phage protein profiles. An important objective was to determine the molecular differences between morphologically similar phages belonging to one family and to find similarities to phages specific for other pathogens. The study also assessed the suitability of the methods used to characterize phages.Electronic supplementary materialThe online version of this article (10.1186/s13568-018-0630-3) contains supplementary material, which is available to authorized users.

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Isolation, characterization, and application of bacteriophage cocktails for the biocontrol of Pseudomonas fluorescens group strains in whole and skimmed milk

Tayyarcan,

Boyaci

2023

Braz J Microbiol

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Characterization and lytic activity of Pseudomonas fluorescens phages from sewage

Cited by 3 publications

References 17 publications

Isolation and Characterization of Lytic Bacteriophages from Wastewater and its Application in Pathogen Reduction

Isolation and Characterization of Lytic Bacteriophages from Wastewater and its Application in Pathogen Reduction

Protein profiles of bacteriophages of the family Myoviridae-like induced on M. haemolytica

Isolation, characterization, and application of bacteriophage cocktails for the biocontrol of Pseudomonas fluorescens group strains in whole and skimmed milk

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