Statistical evaluation of nutritional components impacting phycocyanin production in Synechocystis SP

Deshmukh, Devendra V.; Puranik, Pravin R.

doi:10.1590/s1517-83822012000100041

Cited by 14 publications

(5 citation statements)

References 8 publications

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“…Highest CPC was produced in 100% C-deprived condition and highest APC and PE was found in 50% deprived condition. Some researchers studied the effect of the carbonate source on the phycocyanin pigment production in the Synecocystis spp also (17). It was seen that the effect of lower concentration of carbonate, the phycocyanin content was 13.8 m g/L which on further decrease in concentration of carbonate, was found to be 0.04 g/L and the CPC concentration increased to 16.7m g/L.…”

Section: Effect Of Various Carbonate Concentrations On the Biomass Pr...mentioning

confidence: 99%

Optimization of physico-chemical parameters for the production of phycobilin protein blue pigment, phycocyanin from the cyanobacterial strain Pseudanabaena limnetica (Lemmermann) Komarek

Tribhuvan¹,

Deodhar²,

Kengar³

2023

Plant Sci. Today

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Pseudanabaena limnetica, the cyanophycean microalga, like other members of Cyanophycea, is an excellent source of pigments such as phycocyanin, proteins, carotenoids and polysaccharides. These strains also form a large proportion of algal biomass. The P.limnetica strain can grow in the extreme environmental conditions and it grows well in SW-BG 11 medium under laboratory conditions. In the present investigation, this cyanobacteria strain was isolated from the salt pans of Mulund Mumbai areas and it was cultivated in the lab under controlled conditions of light and temperature with optimum parameters of nitrate and carbonate concentrations. The culture was cultivated in the 60L photo bioreactor systems with the (65,000-85,000 lux) at 45?C in the SW-BG11 medium. The optimization experiments were carried out at the indoor and outdoor conditions. The nitrate and carbonate concentrations were optimized for obtaining maximum amount of algal biomass along with the blue-green phycocyanin pigment. The phycocyanin pigment was lyophilized for its further incorporation into the food and cosmetics products. The spectroscopic calculations of phycocyanin, allophycocyanin and phycoerythrin was done at 620, 650 and 562 nm using the Bennett and Bogorad equation. From the results obtained, it was concluded that 0.1gms/L and 1.5gms/L of the carbonate and nitrate concentrations, respectively, were the ideal concentrations for the further experiments for the cost effective production of P. limnetica in the SW-BG 11 medium. The outdoor conditions were found to be favorable for obtaining the maximum biomass and phycocyanin pigment production which would - make it more cost effective, commercially.

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Section: Effect Of Various Carbonate Concentrations On the Biomass Pr...mentioning

confidence: 99%

Optimization of physico-chemical parameters for the production of phycobilin protein blue pigment, phycocyanin from the cyanobacterial strain Pseudanabaena limnetica (Lemmermann) Komarek

Tribhuvan¹,

Deodhar²,

Kengar³

2023

Plant Sci. Today

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“…1). and Geitlerinema sulphureum at optimal conditions for development of these strains, the phycocyanin concentrations reported were 0.023 and 0.071 mg/mL, respectively [29,30]. An exception was Wilmottia murayi, where the production of phycocyanin was twice higher than that of allophycocyanin; another exception was found in the two strains of Leptolyngbya boryana, where one of them (Leptolyngbya boryana CCALA 084) produced a significant amount of both pigments PC and APC (0.264 and 0.171 mg/mL, respectively) and showed significant potential for use in biotechnological production.…”

Section: Resultsmentioning

confidence: 92%

“…In similar studies with Synechocystis sp. and Geitlerinema sulphureum at optimal conditions for development of these strains, the phycocyanin concentrations reported were 0.023 and 0.071 mg/mL, respectively [29,30].…”

Section: Resultsmentioning

confidence: 99%

Content of phycoerythrin, phycocyanin, alophycocyanin and phycoerythrocyanin in some cyanobacterial strains: Applications

Basheva

Moten

Stoyanov

et al. 2018

Engineering in Life Sciences

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Cyanobacteria are unique prokaryotes, which are capable to perform oxygenic photosynthesis. Within these organisms, phycobilisomes (PBS) act as an antenna of the photosynthetic pigment apparatus. Phycobilisomes contain several phycobiliproteins (PBP): phycoerythrin (PE), phycocyanin (PC), allophycocyanin (APC) and phycoerythrocyanin (PEC). The application of phycobiliproteins in the biotechnology, food industry and medicine during the last years is rapidly increasing. The aim of our study was to assess the qualitative and quantitative content of phycoerythrin, phycocyanin, allophycocyanin and phycoerythrocyanin in 14 cyanobacterial strains kept in Plovdiv Algal Culture Collection (PACC) and 4 strains purchased from the Culture Collection of Autotrophic Organisms (CCALA). Our data demonstrated that three strains of Microcoleus autumnalis (PACC 5505, PACC 5522 and PACC 5527) have high potential to produce phycoerythrins (0.132, 0.201 and 0.136 mg/mL, respectively). Similarly, one Microcoleus autumnalis strain (PACC 5522) and one strain of Leptolyngbya boryana (CCALA 084) are suitable for biotechnological production of phycocyanins (0.051 and 0.264 mg/mL, respectively) as well as allophycocyanins (0.102 and 0.171 mg/mL, respectively). In addition, the data about the pigment content could be used as a biochemical marker for taxonomic purposes within the group.

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“…The method helps to identify the essential components of the medium and to assess their influence on the production rates of secondary metabolites. All the experiments were carried out in triplicate using equation ( 1) (Deshmukh and Puranik, 2012).…”

Section: Optimisation Of the Independent Variables Plackett-burman De...mentioning

confidence: 99%

Medium optimization and downstream process design for the augmented yield of β-Carotene using fungi Blakeslea trispora

Luthra

Babu

Renuka

et al. 2021

PRT

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Purpose β-Carotene is the most appropriate and significant precursor of vitamin A. Synthetic carotene supplements have been known to pose a threat to human health, making natural sources such as the indefensible choice for the production and extraction of carotene. Design/methodology/approach This study considers Blakeslea trispora, a filamentous fungus, as a source of production of carotenoids by fermentation and wet and dry mycelium were used to analyse and obtain better extraction results. Findings In this study, natural oils such as soy oil and cottonseed oil were incorporated into fermentation media to increase the production of carotene. For the optimization process, Plackett–Burman and one-factor-at-a-time (OVAT) models were identified as being of great value. Originality/value OVAT was carried out for corn starch because it plays a major role in the production of carotene and the corn starch at 30 g/L concentration has shown the maximum activity of 3.48 mg/gm. After optimizing process variables, submerged fermentation was eventually carried out under highly controlled media conditions. The resulting product was quantified using UV spectroscopy and extraction of carotene has been observed in the presence of various solvents. Among a range of solvents used, the methylene Di chloride produced-carotene at 86% recovery at a significantly lower temperature of 35°C.

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Statistical evaluation of nutritional components impacting phycocyanin production in Synechocystis SP

Cited by 14 publications

References 8 publications

Optimization of physico-chemical parameters for the production of phycobilin protein blue pigment, phycocyanin from the cyanobacterial strain Pseudanabaena limnetica (Lemmermann) Komarek

Optimization of physico-chemical parameters for the production of phycobilin protein blue pigment, phycocyanin from the cyanobacterial strain Pseudanabaena limnetica (Lemmermann) Komarek

Content of phycoerythrin, phycocyanin, alophycocyanin and phycoerythrocyanin in some cyanobacterial strains: Applications

Medium optimization and downstream process design for the augmented yield of β-Carotene using fungi Blakeslea trispora

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