Statistical optimisation of cell growth and carotenoid production by Rhodotorula mucilaginosa

Maldonade, Iriani Rodrigues; Rodriguez–Amaya, Délia B.; Scamparini, Adilma Regina Pippa

doi:10.1590/s1517-83822012000100012

Cited by 28 publications

(18 citation statements)

References 17 publications

(14 reference statements)

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“…The carotenoid composition was determined according to procedures described by Maldonade et al . Freeze‐dried samples were macerated with Hyflosupercel with a pestle and mortar and exhaustively extracted with acetone.…”

Section: Methodsmentioning

confidence: 99%

“…The carotenoid composition was determined according to procedures described by Maldonade et al 16 Freeze-dried samples were macerated with Hyflosupercel with a pestle and mortar and exhaustively extracted with acetone. The carotenoids were transferred to petroleum ether in a separatory funnel by the addition of water and washed free of acetone.…”

Section: Total Carotenoidsmentioning

confidence: 99%

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Characterization and oxidative stability of oils and bioactive compounds of the fruits of Byrsonima cydoniifolia A. Juss. at different ripening stages

et al. 2019

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BACKGROUND Fruits present high concentrations of bioactive compounds that are beneficial to health due to their antioxidant properties. New alternatives to vegetable oils with such properties have been studied. We determined the chemical compounds of Byrsonima cydoniifolia A. Juss. fruits in three ripening stages as well as the optical behavior and quality level of the oils. RESULTS The ripening stage affected the chemical composition of the fruits and oils. The fruits presented high values of bioactive compounds, as ascorbic acid (1.46–1.82 g kg−1) and total phenols (3.54–15.91 g gallic acid equivalents kg−1), as well as showed excellent antioxidant activity. The ripe fruits showed high content of carotenoids (45.90 mg kg−1) were lutein is the major carotenoid, representing 55.56%. The oil of the ripe fruits showed high lipid content (252.6 g kg−1) and contained oleic (521.83 g kg−1), palmitic (209.13 g kg−1) and linoleic (195.4 g kg−1) fatty acids. The oil of ripe fruits showed the best oxidative stability, with longer induction period (22.29 h) than the other oils. CONCLUSION Fruits of B. cydoniifolia A. Juss. demonstrate to possess a promising potential for the use as functional ingredients. The oil of the ripe fruits was more stable and presents a greater potential to be used for edible purposes. © 2018 Society of Chemical Industry

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Section: Methodsmentioning

confidence: 99%

Section: Total Carotenoidsmentioning

confidence: 99%

Characterization and oxidative stability of oils and bioactive compounds of the fruits of Byrsonima cydoniifolia A. Juss. at different ripening stages

et al. 2019

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“…R. mucilaginosa is commonly found in humans, animals, food and beverages (Wirth and Goldani, 2012;Deligios et al, 2015). Over the years, this species has been widely studied for its carotenoids production (Aksu and Eren, 2005;Maldonade et al, 2012). The ability of this yeast to accumulate high oil content has also been reported, but most of these studies only highlighted its substrate utilization Reyna-Martinez et al, 2015;Gientka et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Development of Rhodotorula mucilaginosa strain via random mutagenesis for improved lipid production

Wong¹,

Vincent²

2019

MJM

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Aims:Oleaginous yeasts are widely used for the production of biodiesel feedstocks because of their high lipid content. This research was aimed to conduct random mutagenesis of Rhodotorula mucilaginosa using ethyl methane sulfonate (EMS) and identify the mutants with improved lipid production. Methodology and results: A total of twenty-two mutant isolates prescreened with cerulenin were produced and further characterized via M13 PCR fingerprinting to determine their polymorphism and genetic distances. Eight strains, namely M1, M2, M3, M4, M7, M10, M11 and M18, were chosen based on their genetic distances from the parental strain for biomass production. Six mutants (M1, M2, M3, M4, M7 and M18) showing the highest dry cell weights were further selected for evaluation of lipid production in a laboratory-scale bioreactor using glucose as a carbon source. Results indicated that parental strain exhibited lipid content of 1.83 g/L, while strains M1, M2, M3, M7 and M18 generated 2.37 g/L, 2.27 g/L, 2.27 g/L, 3.10 g/L and 3.83 g/L of intracellular lipid, respectively. These five mutants were identified to have significant increase in lipid production compared to the parental strain. Conclusion, significance and impact of study: This study demonstrated enhanced lipid production in R. mucilaginosa by random mutagenesis. New generated strains had higher lipid productivity compared to parental strain and application of these strains in industry may reduce the overall cost of biodiesel production.

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“…Accordingly, a number of strains of R . mucilaginosa have been explored for the commercial production of carotenoids, mainly β ‐carotene, torulene and torularhodin (Aksu and Eren, ; Maldonade et al ., ), for liquid bioremediation processes (Jarboui et al ., ), for heavy metal biotransformation (Rajpert et al ., ) and for their antifungal and plant growth‐promoting actions (Ignatova et al ., ). In addition, there is also interest concerning their role as emergent opportunistic pathogens in immunocompromised individuals (Wirth and Goldani, ).…”

Section: Introductionmentioning

confidence: 99%

Proteomic analysis of Rhodotorula mucilaginosa: dealing with the issues of a non‐conventional yeast

Addis

Tanca

Landolfo

et al. 2016

Yeast

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Red yeasts ascribed to the species Rhodotorula mucilaginosa are gaining increasing attention, due to their numerous biotechnological applications, spanning carotenoid production, liquid bioremediation, heavy metal biotransformation and antifungal and plant growth-promoting actions, but also for their role as opportunistic pathogens. Nevertheless, their characterization at the 'omic' level is still scarce. Here, we applied different proteomic workflows to R. mucilaginosa with the aim of assessing their potential in generating information on proteins and functions of biotechnological interest, with a particular focus on the carotenogenic pathway. After optimization of protein extraction, we tested several gel-based (including 2D-DIGE) and gel-free sample preparation techniques, followed by tandem mass spectrometry analysis. Contextually, we evaluated different bioinformatic strategies for protein identification and interpretation of the biological significance of the dataset. When 2D-DIGE analysis was applied, not all spots returned a unambiguous identification and no carotenogenic enzymes were identified, even upon the application of different database search strategies. Then, the application of shotgun proteomic workflows with varying levels of sensitivity provided a picture of the information depth that can be reached with different analytical resources, and resulted in a plethora of information on R. mucilaginosa metabolism. However, also in these cases no proteins related to the carotenogenic pathway were identified, thus indicating that further improvements in sequence databases and functional annotations are strictly needed for increasing the outcome of proteomic analysis of this and other non-conventional yeasts.

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Statistical optimisation of cell growth and carotenoid production by Rhodotorula mucilaginosa

Abstract: Sequential statistical methods were used to maximise carotenoid production by a strain of Rhodotorula mucilaginosa, isolated from the Brazilian ecosystem. Initially, a factorial 2 5-1 experimental design was used, and the variables were pH and the levels of glucose, yeast extract,

Cited by 28 publications

References 17 publications

Characterization and oxidative stability of oils and bioactive compounds of the fruits of Byrsonima cydoniifolia A. Juss. at different ripening stages

Characterization and oxidative stability of oils and bioactive compounds of the fruits of Byrsonima cydoniifolia A. Juss. at different ripening stages

Development of Rhodotorula mucilaginosa strain via random mutagenesis for improved lipid production

Proteomic analysis of Rhodotorula mucilaginosa: dealing with the issues of a non‐conventional yeast

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