Biosynthesis, purification and characterization of endoglucanase from a xylanase producing strain Aspergillus niger B03

Dobrev, Georgi; Zhekova, Boriana

doi:10.1590/s1517-83822012000100008

Cited by 9 publications

(2 citation statements)

References 20 publications

(19 reference statements)

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“…BcsZ could hydrolyze CMC-Na, RAC and glucan. BcsZ had a higher affinity for CMC than Thcel9A (12.02 mg/mL) from Thermobifida halotolerans YIM 90462 T [25], the purified endoglucanase (21.01 mg/mL) from Aspergillus niger B03 [26] and C67–1 (37 mg/mL) from metagenomes of buffalo rumens [27]. It had a weaker affinity for CMC than the endoglucanase from Bacillus sp.…”

Section: Discussionmentioning

confidence: 99%

Identification and characterization of an Endo-glucanase secreted from cellulolytic Escherichia coli ZH-4

et al. 2019

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Background In the previous study, the cellulolytic Escherichia coli ZH-4 isolated from bovine rumen was found to show extracellular cellulase activity and could degrade cellulose in the culture. The goal of this work was to identify and characterize the secreted cellulase of E. coli ZH-4. It will be helpful to re-understand E. coli and extend its application in industry. Results A secreted cellulase was confirmed to be endo-glucanase BcsZ which was encoded by bcsZ gene and located in the cellulose synthase operon bcsABZC in cellulolytic E. coli ZH-4 by western blotting. Characterization of BcsZ indicated that a broad range of pH and temperature tolerance with optima at pH 6.0 and 50 °C, respectively. The apparent Michaelis–Menten constant (K m ) and maximal reaction rate (V max ) for BcsZ were 8.86 mg/mL and 0.3 μM/min·mg, respectively. Enzyme activity of BcsZ was enhanced by Mg 2+ and inhibited by Zn 2+ , Cu 2+ and Fe 3+ . BcsZ could hydrolyze carboxymethylcellulose (CMC) to produce cello-oligosaccharides, cellotriose, cellobiose and glucose. Conclusions It is confirmed that extracellular cellulolytic capability of E. coli ZH-4 was attributed to BcsZ, which explained why E. coli ZH-4 can grow on cellulose. The endo-glucanase BcsZ from E. coli -ZH4 has some new characteristics which will extend the understanding of endo-glucanase. Analysis of the secretion characteristics of BcsZ provided a great reference for applying E. coli in multiple industrial fields.

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Section: Discussionmentioning

confidence: 99%

Identification and characterization of an Endo-glucanase secreted from cellulolytic Escherichia coli ZH-4

et al. 2019

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“…This result was attributed to the higher level of hydrolysis of CMC compared with the other substrates. Endoglucanase has high substrate specificity for CMC and lower specificity for crystalline forms of cellulose (Kim 1995;Dobrev and Zhekova 2012;Miotto et al 2014). Hence, endoglucanase, produced by the medium, must have hydrolysed the CMC better, thus producing higher amounts of reducing sugar than the other substrates.…”

Section: Reducing Sugar Productionmentioning

confidence: 99%

Enhanced Endoglucanase Production by Bacillus aerius on Mixed Lignocellulosic Substrates

2016

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Selected carbon sources including soluble carboxymethyl cellulose (CMC), insoluble microcrystalline cellulose (MCC), and single (SS)/mixed lignocellulosic substrates (MS), were evaluated for endoglucanase production by B. aerius S5.2. The lignocellulosic substrates of oil palm empty fruit bunch (EFB), oil palm frond (OPF), rice husk (RH), and their mixture (MS) supported growth of the strain better than CMC and MCC. The maximum endoglucanase activity on MS was 7.3-, 2.6-, 1.7-, and 1.2-fold higher than those recorded on MCC, CMC, EFB/OPF, and RH, respectively. While the reducing sugar concentration of the CMC medium was comparable to that of the EFB and MS media, wide variability was observed in the reducing sugar concentrations among the lignocellulosic substrates. Extremely low levels of sugar were detected in the MCC medium, reflecting its poor digestibility and hence unsuitability for growth and endoglucanase production. Endoglucanase production was predominantly extracellular when the strain was grown on CMC and MS. After seven days of fermentation, there was an approximately 25% reduction in MS dry weight. These findings show that the use of mixed lignocellulosics could potentially reduce the cost of cellulase production. Certain novel aspects of the cellulase system of B. aerius are reported in this study.

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Purification, Characterization, and Application of Endoglucanase from Rhizopus oryzae as Antibiofilm Agent

Karmakar

Lahiri

Nag

et al. 2022

Appl Biochem Biotechnol

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Biosynthesis, purification and characterization of endoglucanase from a xylanase producing strain Aspergillus niger B03

Cited by 9 publications

References 20 publications

Identification and characterization of an Endo-glucanase secreted from cellulolytic Escherichia coli ZH-4

Identification and characterization of an Endo-glucanase secreted from cellulolytic Escherichia coli ZH-4

Enhanced Endoglucanase Production by Bacillus aerius on Mixed Lignocellulosic Substrates

Purification, Characterization, and Application of Endoglucanase from Rhizopus oryzae as Antibiofilm Agent

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