Application of response surface methodology to enhancement of biomass production by Lactobacillus rhamnosus E/N

Abstract

Response surface methodology (RSM) was employed to study the effects of various medium components on biomass production by Lactobacillus rhamnosus E/N. This strain is commonly used in the pharmaceutical and food industries due to its beneficial effect on the human gut and general health. The best medium composition derived from RSM regression was (in g/l) glucose 15.44, sodium pyruvate 3.92, meat extract 8.0, potassium phosphate 1.88, sodium acetate 4.7, and ammonium citrate 1.88. With this medium composition biomass production was 23 g/l of dry cell weight after 18 h of cultivation in bioreactor conditions, whereas on MRS the yield of biomass was 21 g/l of dry cell weight. The cost of 1 g of biomass obtained on MRS broth was calculated at the level of 0.44 € whereas on the new optimal medium it was 25% lower. It may be concluded then, that the new medium, being cheaper than the control MRS allows large scale commercial cultivation of the L. rhamnosus strain. This study is of relevance to food industry because the possibility to obtain high yield of bacterial biomass is necessary step in manufacturing of probiotic food.

High-Throughput Optimization of Effective Microbes Nutrient Formulation via Automated Media Optimization System (AMOS)

“…3). It can be concluded that both tested nutrients provide greater bacterial growth performance as well as bacteria biomass productivity, hence, in agreement to research by Polack-Berecka [11] and Lei Yu [23] that additionally enhanced the production of bacteriocin produced by lactic acid bacteria although pediocin activity and yield determination were not conducted in this study.…”

“…According to Fung [21], meat extract has a significant impact on the growth of L. acidophilus. Furthermore, Polak-berecka [11] study showed that a complex nitrogen source is vital for the growth of lactic acid bacteria. They also highlight that meat extract is label as the best complex nitrogen source compared to other nitrogen sources, in example peptone and yeast extract.…”

“…They also reported that the production of pediocin activity of P. acidilactici reached its peak casein peptone medium. According to Polak-Berecka [11], media supplemented with meat extract yielded a slightly higher dry weight compared to other media supplemented with yeast extract and peptone. This is because the amount of total nitrogen percentage per weight is greater in beef extract than the other sources.…”

Production of Lactobacillus rhamnosus BRM 029693 in feed-batch fermentation

“…Source: Authors, Hwang et al (2015) used a similar culture medium (10 g/L glucose, 10 g/L yeast extract, 10 g/L soy peptone, 2 g/L ammonium citrate, 0.2 g/L magnesium sulfate, 5 g/L sodium acetate, 0.05 g/L manganese sulfate, and supplementation with 10 ml/L corn syrup) to Research, Society and Development, v. 9, n. 7, e531974280, 2020 (CC BY 4.0) | ISSN 2525-3409 | DOI: http://dx.doi.org/10.33448/rsd-v9i7.4280 the simplified MRS in producing Lactobacillus acidophilus cells and obtained a maximum biomass production of 4.54 g/L in dry weight. Berecka et al (2011) studied the effect of various components of the culture medium on Lactobacillus rhamnosus biomass production and determined that a medium composed of 15.44 g/L glucose, 3.92 g/L sodium pyruvate, 8.0 g/L meat extract, 1.88 g/L potassium phosphate, 4.7 g/L sodium acetate and 1.88 g/L ammonium citrate resulted in 23 g/L of dry matter biomass after 18 hours of cultivation in a bioreactor, with a mixed source of carbon, glucose and sodium pyruvate being the preferred substrate for the growth of this microorganism.…”

Multivariate optimization of Staphylococcus xylosus AD1 biomass production using sugarcane molasses plus yeast extract and soybean meal

“…), and for this its efficient utilization by microbial cell should not be taken for granted (Basso, Basso, & Rocha, 2011;Lino, Basso, & Sommer, 2018). Considering that multivariate optimization can be used to evaluate the relative significance of several factors in the presence of complex interactions (Polak-Berecka, Waśko, Kordowska-Wiater, Targoński, & Kubik-Komar, 2011), in this study a Box Behnken multivariate design was employed with the aim to develop a culture medium based on sugarcane molasses (SCM) supplemented with yeast extract (YE) and soybean meal (SM) for the production of S. xylosus AD1 biomass.…”