Phylogenetic characterization of bovine parainfluenza 3 from contaminated cell cultures and field isolates from Brazil

Vaucher, Rodrigo de Almeida; Dezen, Diogenes; Simonetti, Amauri Braga; Spilki, Fernando Rosado; Roehe, Paulo Michel

doi:10.1590/s1517-83822011000400029

Cited by 6 publications

(5 citation statements)

References 14 publications

(17 reference statements)

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“…Viral abortions in cattle are reported in low frequencies in several studies (Vaucher et al 2011, Clothier & Anderson 2016. In this case series, one abortion was caused by BPI-3 virus.…”

Section: Discussionmentioning

confidence: 60%

Causes of abortion in dairy cows in Uruguay

et al. 2020

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A case series study was conducted to determine the frequency of causes of abortion in dairy cattle in Uruguay. The sample size of 102 cases was composed of 53 fetuses, 35 fetuses with placentas, and 14 placentas without an associated fetus. All cases underwent gross and microscopic pathologic examinations as well as microbiological and serological testing. The etiology was determined in 54 (53%) of cases, 51 of which were caused by infectious agents. Within the observed 102 cases, 30 (29%) were caused by Neospora caninum, six (6%) by Coxiella burnetii and two (2%) by Campylobacter fetus subsp. venerealis. Bovine Parainfluenza-3 virus and Salmonella enterica serovar Newport caused one abortion each. Opportunistic bacteria (Escherichia coli, Streptococcus sp., Staphylococcus sp., Mannheimia sp., Trueperella pyogenes, and Providencia stuartii) were associated with 11 abortions. In two cases the fetal death was attributed to dystocia, and in one case the fetus had a congenital mesothelioma. Bovine viral diarrhea virus (BVDV) infection was identified in three fetuses; two of which were co-infected with and had typical lesions of N. caninum. No lesions were observed in the other fetus infected by BVDV. Leptospira interrogans was identified in one fetus without lesions. Despite the relatively low overall success rate in establishing an etiological diagnosis in cases of abortion in cattle, a systemic workup of bovine abortion is necessary to establish prevention and control strategies. This also facilitates monitoring and surveillance of reproductive diseases in dairy cattle, some of which represent a risk to public health.

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“…Viral abortions in cattle are reported in low frequencies in several studies (Vaucher et al 2011, Clothier & Anderson 2016. In this case series, one abortion was caused by BPI-3 virus.…”

Section: Discussionmentioning

confidence: 60%

Causes of abortion in dairy cows in Uruguay

et al. 2020

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“…In case series from South America, including Uruguay, 7 Brazil, 5 and Argentina, 2 that collectively evaluated several hundred laboratory submissions, BPIV-3 was not reported as a cause of bovine abortion. However, BPIV-3a was isolated from an aborted bovine fetus in Brazil, 6 although no pathologic information was provided.…”

mentioning

confidence: 99%

“…hundred laboratory submissions, BPIV-3 was not reported as a cause of bovine abortion. However, BPIV-3a was isolated from an aborted bovine fetus in Brazil, 6 although no pathologic information was provided.…”

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confidence: 99%

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Fetal Pathology in an Aborted Holstein Fetus Infected With Bovine Parainfluenza Virus-3 Genotype A

et al. 2018

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Bovine parainfluenza virus-3 (BPIV-3) is a recognized respiratory pathogen of cattle, and it has also been identified in aborted fetuses. However, little is known of this agent as a reproductive pathogen and detailed descriptions of fetal pathology on natural cases are lacking in the scientific literature. This article describes and illustrates lesions in a fetus spontaneously aborted by a first-calving Holstein heifer, naturally infected with BPIV-3 genotype A, broadening the current knowledge on fetal pathology by this virus. Fetal autopsy revealed diffusely reddened, rubbery and unexpanded lungs. Histologically, there was necrotizing bronchiolitis/alveolitis with intraluminal fibrin exudate and syncytial cells in the bronchiolar/alveolar spaces, and non-suppurative peribronchiolitis and perivascular interstitial pneumonia. In the small intestine there was multifocal necrotizing cryptitis and occasional necrotic syncytial enterocytes. Intralesional and extralesional BPIV-3 antigen was detected by immunohistochemistry in the lung and small intestine, and BPIV-3a was identified in fetal tissues by RT-PCR and sequencing.

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“…Viruses HAdV, HRV, FLUAV, RSV, and RV have been in house assay, according to Menezes [38] , Demoliner [39] and Bortagaray [40] . HPIV was determined by RT-qPCR, as described by Vaucher [41] . HSV-1 and HSV-2 were determined by PCR as described by Sartori [42] .…”

Section: Methodsmentioning

confidence: 99%