Modification of c and n sources for enhanced production of cyclosporin 'a' by Aspergillus Terreus

Abstract

Most of the studies regarding cyclosporin 'A' production through fungi concentrate around Tolypocladium inflatum. This is mainly due to lower reported production of this drug in other fungi. The present study was therefore conducted to explore indigenous isolates of Aspergillus terreus for synthesis of this drug and defining a production medium for obtaining high yield of cyclosporin 'A'. For this purpose carbon and nitrogen sources were optimized for the selected best strain of A. terreus. Overall results depicted that the best cyclosporin 'A' yield from selected Aspergillus terreus (FCBP58) could be obtained by using production medium containing glucose 10% as carbon source and peptone 0.5% as nitrogen source. This modification in production medium enhanced drug synthesis by selected fungi significantly. The production capabilities when compared with biomass of fungi there was found no relationship between the two confirming that the medium modification increased overall drug synthesis powers of the fungi.

Transfigured Morphology and Ameliorated Production of Six Monascus Pigments by Acetate Species Supplementation in Monascus ruber M7

“…This argument is supported by a previous study in which different salts and media were used and fungi showed almost same pattern of growth as given in this study. At low concentration, the biomass increased but with increase in salt concentration decreased the biomass of Aspergillus terreus [40]. Furthermore, different salts used in media affected the biomass of Fusarium oxysporum and Aspergillus nidulans [41].…”

“…Experiments performed to investigate the role of cellulose and lactose showed freely dispersed mycelia and entangled mycelia in Trichoderma reesei [46]. Another research showed that the biomass of A. terreus is significantly affected by changes in salt concentration [40]. Furthermore, the biomass of F. oxysporum and Aspergillus nidulans is also affected by use of salts in culture media [41].…”

Comparative proteomics reveals the mechanism of cyclosporine production and mycelial growth in Tolypocladium inflatum affected by different carbon sources

“…Zaleski in a fructosecontaining medium, and the culture reached the maximum between days 12 and 15 (Pessoni et al, 2015). Some studies have attempted to replace CsA-producing strains with selected Aspergillus terreus Thom (FCBP58), and the CsA yield was effectively increased by optimizing the ratio of the carbon source to the nitrogen source in the medium (Tanseer and Anjum, 2011). However, few studies have analyzed how carbon sources affect the production of secondary metabolite CsA and its producing fungal mycelium growth.…”