An in-house multiplex pcr method to detect of putative virulence factors in Aeromonas species

Aguilera‐Arreola, Ma. Guadalupe; Martínez, Alma Aidee Carmona; Castro-Escarpulli, Graciela

doi:10.1590/s1517-83822011000400011

Cited by 2 publications

(2 citation statements)

References 14 publications

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“…The five most important virulence factors of Aeromonas spp. are believed to be lateral flagella, the cytolytic enterotoxins ast and alt, aerolysins and haemolysins and GCAT (Aguilera-Arreola, Martinez, & Castro-Escarpulli, 2011).…”

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Diagnostic methods for identifying different Aeromonas species and examining their pathogenicity factors, their correlation to cytotoxicity and adherence to fish mucus

Jung‐Schroers

Jung

Ryll

et al. 2018

Journal of Fish Diseases

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Aeromonas spp. are ubiquitous in the aquatic environment, acting as facultative or obligate pathogens for fish. Identifying Aeromonas spp. is important for pathogenesis and prognosis in diagnostic cases but can be difficult because of their close relationship. Forty‐four already characterized isolates of Aeromonas spp. were analysed by 16S rRNA gene sequencing, by gyrase B sequencing, by analysing their fatty acid profiles, by biochemical reactions and by MALDI‐TOF MS. To determine their pathogenicity, cytotoxicity, adhesion to mucus and the expression of 12 virulence factors were tested. The susceptibility of the isolates towards 13 different antibiotics was determined. MALDI‐TOF MS was found to be an acceptable identification method for Aeromonas spp. Although the method does not detect all species correctly, it is time‐effective and entails relatively low costs and no other methods achieved better results. A high prevalence of virulence‐related gene fragments was detected in almost all examined Aeromonas spp., especially in A. hydrophila and A. salmonicida, and most isolates exhibited a cytotoxic effect. Single isolates of A. hydrophila and A. salmonicida showed multiple resistance to antibiotics. These results might indicate the potentially pathogenic capacity of Aeromonas spp., suggesting a risk for aquatic animals and even humans, given their ubiquitous nature.

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confidence: 99%

Diagnostic methods for identifying different Aeromonas species and examining their pathogenicity factors, their correlation to cytotoxicity and adherence to fish mucus

Jung‐Schroers

Jung

Ryll

et al. 2018

Journal of Fish Diseases

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“…It was also decided to include Aeromonas, because some species have a broad host spectrum, causing intestinal and extra-intestinal infections in humans. Besides, in Mexico, it has been shown in the last fifteen years that Aeromonas represent an important group of pathogens involved in diarrhea (Castro-Escarpulli et al, 2003;Aguilera-Arreola et al, 2005;Aguilera-Arreola et al, 2011).…”

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confidence: 99%

Design and standardization of four multiplex polymerase chain reactions to detect bacteria that cause gastrointestinal diseases

Hernández-Cortez¹,

Méndez‐Tenorio²,

Ma.Guadalupe³

et al. 2013

Afr. J. Microbiol. Res.

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Gastrointestinal diseases and diarrhea are the major cause of morbidity and mortality in children in the developing world, including Mexico. In order to diagnose acute diarrhea and dysentery, four-multiplex polymerase chain reaction (mPCR) sets were designed and standardized in this study. Main virulence genes of relevant enteropathogens, that is, Escherichia coli pathotypes, Shigella spp., Salmonella enterica, Yersinia enterocolitica, Campylobacter jejuni and Aeromonas spp., were detected. Sixteen primer pairs were designed using extensive in silico analysis. As predicted in vitro, specific amplicons were obtained and four multiplex PCR sets were standardized. Our sixteen primer pairs and four multiplex PCR results demonstrate the simultaneous amplification of different pathogens according to the type of diarrhea in the same reaction. In the future, these methods might be used as rapid, sensitive and specific epidemiological and diagnostic tools.

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An in-house multiplex pcr method to detect of putative virulence factors in Aeromonas species

Cited by 2 publications

References 14 publications

Diagnostic methods for identifying different Aeromonas species and examining their pathogenicity factors, their correlation to cytotoxicity and adherence to fish mucus

Diagnostic methods for identifying different Aeromonas species and examining their pathogenicity factors, their correlation to cytotoxicity and adherence to fish mucus

Design and standardization of four multiplex polymerase chain reactions to detect bacteria that cause gastrointestinal diseases

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