Mutacins of Streptococcus mutans

Kamiya, Regianne Umeko; Taiete, Tiago; Gonçalves, Reginaldo Bruno

doi:10.1590/s1517-83822011000400001

Cited by 22 publications

(14 citation statements)

References 60 publications

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“…Mutacins also represent an important virulence factor associated with the risk of dental caries. The adsorption capacity of active molecules of mutacins on the surface of sensitive bacteria through specific or not specific receptors can increase their antimicrobial efficiency (8). Furthermore, when a child is exposed to infection by an S. mutans strain exhibiting an increased level of mutacin production, it can be presumed that under favorable circumstances the strain will colonize, especially if the flora has not yet reached stability (9).…”

Section: Introductionmentioning

confidence: 99%

Virulence of Streptococcus mutans: An intrafamilial cohort study on transmission of genotypes

Cota¹,

Silva²,

Silva³

2020

J Clin Exp Dent

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Background: The main aims of this cohort study were to measure the intrafamilial risk of transmission, sharing and stability of the most virulent S. mutans genotypes. Material and Methods: A total of 392 clinical isolates of S. mutans obtained from caries-active adults and genotyped to evaluate their transmissibility over time. After extraction of the chromosomal DNA, PCR were performed to detect the genes involved in the production of GbpA (gbpA) and mutacin types I, II, III and IV (mutAI, mutAII, mutAIII and mutAIV). Results: The gbpA, mutAI, mutAII, mutAIII and mutAIV genes were detected in 77.3, 12.5, 51, 16.6 and 89.8% of S. mutans isolates, respectively. The virulence of S. mutans was associated with its transmission (P < 0.01) and stability (P = 0.01), with the most virulent genotypes having higher transmissibility (RR = 1.83, 95% CI 1.44 to 2.32) and higher stability in the oral cavity (RR = 1.52, 95% CI 1.06 to 2.19). Conclusions: Genotypes with the genetic information to synthesize GbpA and mutacins present an important ecological advantage in the process of colonization by S. mutans; they remain stable among the oral microbiota of the host and favor intrafamilial transmission.

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Section: Introductionmentioning

confidence: 99%

Virulence of Streptococcus mutans: An intrafamilial cohort study on transmission of genotypes

Cota¹,

Silva²,

Silva³

2020

J Clin Exp Dent

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“…Due to its multifactorial nature and microbial origin, the severity and prevalence of caries can be greatly affected by the endogenous conditions within each host individual 2 . Several studies have demonstrated that the colonization and accumulation of Streptococcus mutans is associated with dental caries in humans, since they are influenced by various factors in the oral cavity, such as nutrition and hygiene conditions of the host, salivary components, cleaning power and salivary flow 236 236 236 236 and characteristics related with microbial virulence factors 3 . Streptococcus mutans is the key contributor to the formation of polysaccharide-based extracellular matrices in dental biofilms 4 .…”

Section: Introductionmentioning

confidence: 99%

Using molecular markers to assess Streptococcus mutans variability and the biological risk for caries

Neiva¹,

Moreira²,

Gomes³

et al. 2014

Braz. J. Oral Sci.

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Aim: To characterize the genetic variability of Streptococcus mutans isolates and to correlate this variability with different colonization profiles observed during dental caries in a sample of children. Methods: S. mutans samples were isolated from the saliva of 30 children with varying histories of dental caries, and they were characterized according to morphological and biochemical markers and the sequences of their 16S-23S intergenic spacer region. The genetic variability of the isolates was first assessed using Random Amplified Polymorphic DNA (RAPD) markers. Next, the isolates were differentiated by sequencing a specific region of the gene encoding the enzyme glucosyltransferase B (gtfB). Results: Characterization using RAPD markers uncovered significant genetic variability among the samples and indicated the existence of clusters, which allowed us to reconstruct both the origin and clinical history of the disease. By sequencing the 16S-23S intergenic region, it was found that all of the isolates belonged to the species S. mutans. Based on the genetic similarity of the isolates and pattern of amino acid variations identified by partial sequencing of the gtfB gene, base-pair changes were identified and correlated with different virulence patterns among the isolates. Conclusions: The partial sequencing of the gtfB gene can be a useful tool for elucidating the colonization patterns of S. mutans. As amino acid variations are likely to be correlated with differences in biological risk, molecular characterization, such as that described in this paper, could be the key for assessing the development of dental caries in children.

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“…Elas representam um importante fator de virulência relacionado com o risco de cárie dentária (KURAMITSU, 1993;GRÖNROOS et al, 1998;NAPIMOGA et al, 2004). A capacidade de uma cepa bacteriana produzir mutacinas interfere na invasão e proliferação de outras bactérias no biofilme, aumentando seu potencial cariogênico e conferindo significativa vantagem ecológica para a cepa produtora (ROGERS; van der HOEVEN; MIKX, 1979;JOHNSON;YAPHE, 1984;BALAKRISHNAN et al, 2002;KRETH et al, 2006;NICOLAS;LAPOINTE, 2007;NGUYEN et al, 2009;KAMYIA;TAIETE;GONÇALVES, 2011).…”

Section: V4unclassified

“…Sua produção é rigorosamente controlada por mecanismos regulatórios (TSANG et al, 2005) e pode ser desencadeada diante de uma densa e complexa colonização do biofilme. Isso sugere que sua atuação seja, predominantemente, nas etapas tardias de infecção por S. mutans CAULFIELD, 2001;KAMYIA;TAIETE;GONÇALVES, 2011).…”

Section: V4unclassified

“…Grönroos et al (1998) destacaram que quando uma criança é exposta à infecção por uma cepa de S. mutans que exibe aumento do nível de produção de mutacinas, pode-se presumir que, sob circunstâncias favoráveis, a mesma vai ser hábil em colonizar o hospedeiro, especialmente se a microbiota bucal ainda não tiver atingido sua estabilidade. Acredita-se que a mutacinogênese influencie a implantação e colonização de S. mutans por meio da competição entre as espécies bacterianas, substituindo a microbiota residente ou excluindo bactérias exógenas (HILLMAN; JOHNSON;YAPHE, 1984;KAMYIA;TAIETE;GONÇALVES, 2011;LONGO;MAYER, 2003;KURAMITSU et al, 2007). Nesse sentido, há mais de 20 anos, alguns autores (BERKOWITZ; JORDAN, 1975;JOHNSON;YAPHE, 1984;ALALUUSUA, 1991) já haviam proposto que a propriedade biológica de antagonismo seletivo das mutacinas talvez ajude a explicar por que, uma vez que S. mutans se estabeleçam, eles são dificilmente eliminados da microbiota bucal, tornando-se prevalentes na saliva, biofilme e lesões de cárie dentária.…”

Section: Estudo Pilotounclassified

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Fatores de virulência de Streptococcus mutans e sua relação com a persistência e transmissão de genótipos entre membros de famílias brasileiras

Cota¹

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Mutacins of Streptococcus mutans

Cited by 22 publications

References 60 publications

Virulence of Streptococcus mutans: An intrafamilial cohort study on transmission of genotypes

Virulence of Streptococcus mutans: An intrafamilial cohort study on transmission of genotypes

Using molecular markers to assess Streptococcus mutans variability and the biological risk for caries

Fatores de virulência de Streptococcus mutans e sua relação com a persistência e transmissão de genótipos entre membros de famílias brasileiras

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