Identification and biological activity of potential probiotic bacterium isolated from the stomach mucus of breast-fed lamb

Sepová, Hana Kiňová; Dubničková, M; Bilková, Andrea; Bukovský, M; Bezáková, L

doi:10.1590/s1517-83822011000300044

Cited by 4 publications

(1 citation statement)

References 2 publications

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“…Four studied L. reuteri isolates originated from lamb and goatling were identified on species level previously and are the subject of research for their potential probiotic properties. All strains assigned diverse phenotypical features Kiňová Sepová et al 2011;Kiňová Sepová and Bilková 2013).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of biochemical and molecular methods for Lactobacillus reuteri strains differentiation

et al. 2014

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Several biochemical and molecular methods were used for discrimination of four Lactobacillus reuteri strains isolated from goatling and lamb stomach mucosa. Internal transcribed spacer (ITS)-PCR method and protein analysis by SDS-PAGE and MALDI-TOF showed to be suitable for strain discrimination whereas ITS-PCR/RFLP and enterobacterial repetitive intergenic consensus (ERIC)-PCR were not strain specific. The used methods differentiated tested strains into distinct groups; however, the location of strains in groups varied. Consistency in results was observed in the case of L. reuteri E and L. reuteri KO4m that were clustered into the same groups using all techniques, except of MALDI-TOF MS. The last one grouped goatling strains and lamb isolate into separate clusters. All investigated methods, except of ITS-PCR/RFLP and ERIC-PCR, were assessed as appropriate for distinguishing of L. reuteri strains.

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Section: Introductionmentioning

confidence: 99%

Evaluation of biochemical and molecular methods for Lactobacillus reuteri strains differentiation

et al. 2014

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Evaluation of adhesion properties of lactobacilli probiotic candidates

et al. 2018

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Isolation and identification of new lactobacilli from goatling stomach and investigation of reuterin production in Lactobacillus reuteri strains

Sepová

Bilková

2012

Folia Microbiol

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Five new strains of lactobacilli isolated from goatling's stomach were identified by molecular-biological approaches. Profiles of fermentable saccharides, Gram staining, and cell morphology were also determined. They were identified as Lactobacillus reuteri (strains KO4b, KO4m, KO5) and as Lactobacillus plantarum (strains KG1z, KG4). In DNA samples of all newly isolated L. reuteri strains as well as in L. reuteri E (Lreu E; originated from lamb), the part of gldC gene, coding large subunit of glycerol dehydratase, that is necessary for 3-hydroxypropionaldehyde (3-HPA; reuterin) production, was amplified using two designed primer sets. However, the 3-HPA production was revealed only in the strain Lreu E. It produced five- or ten-fold lower amount of 3-HPA in comparison with probiotic L. reuteri ATCC 55730 in aerobic or anaerobic conditions, respectively. Moreover, Lreu E completely lost its production ability after ca. five passages in MRS medium. The co-incubation of Lreu E, but not other L. reuteri isolates, with Escherichia coli re-induced 3-HPA production. In the case of L. reuteri ATCC 55730, the 3-HPA production increased more than four times after co-incubation with E. coli.

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Identification and biological activity of potential probiotic bacterium isolated from the stomach mucus of breast-fed lamb

Cited by 4 publications

References 2 publications

Evaluation of biochemical and molecular methods for Lactobacillus reuteri strains differentiation

Evaluation of biochemical and molecular methods for Lactobacillus reuteri strains differentiation

Evaluation of adhesion properties of lactobacilli probiotic candidates

Isolation and identification of new lactobacilli from goatling stomach and investigation of reuterin production in Lactobacillus reuteri strains

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