Medium composition influence on Biotin and Riboflavin production by newly isolated Candida sp

Abstract

Complex B vitamins as Biotin and Riboflavin are required by living organisms, not only for growth but also for metabolite production, and the feed market classifies them as growth promoters. Since Brazil will soon be one of the world's biggest animal protein producers, feed production is a large consumer of vitamins and micronutrients. The industry requires 10 mg riboflavin/0.2 mg biotin per kilogram of feed; a ratio of 40 ~ 50:1. Although few studies have been conducted specifically on riboflavin production using factorial design and surface response method as an optimization strategy, it is a common practice in biotechnology with many research reports available. However, there are no reports on the use of statistical design for biotin production. This study set out to evaluate medium composition influence on biotin and riboflavin production using a statistical design. There are no studies relating biotin and riboflavin production by Candida sp LEB 130. In this preliminary study to improve the simultaneous production of biotin and riboflavin, the maximum riboflavin/biotin ratio of 8.3 µg/mL was achieved with medium component concentrations of: sucrose 30 g/L, KH 2 PO 4 2 g/L, MgSO 4 1 g/L and ZnSO 4 0.5mL/L.

Production of Vitamin B2 (Riboflavin) by Microorganisms: An Overview

“…Attempts to improve microbial riboflavin-producing strains were made by both metabolic and genetic engineering, which include the following: (1) random mutagenesis by chemical exposure and UV irradiation (Matsuyama et al, 1987;Park et al, 2007;Kavitha and Chandra, 2014); (2) random and site-directed mutagenesis by genetically engineered deletions, insertions, or substitutions (Monschau et al, 1998;Zamboni et al, 2003;Jiménez et al, 2005Jiménez et al, , 2008Zhu et al, 2006;Kato and Park, 2012;Lin et al, 2014;Ledesma-Amaro et al, 2015;Wang et al, 2015;Liu et al, 2019;Lu et al, 2019); (3) selection (Schmidt et al, 1996); and (4) optimization of the fermentation process depending on medium components and their concentrations (Leathers and Gupta, 1997;Kalingan and Liao, 2002;Lim et al, 2003;Pessoa et al, 2003;Wu et al, 2007;Sugimoto et al, 2010;Cheng et al, 2011;Suzuki et al, 2011). Duplications, insertions, deletions, modifications, substitutions, upregulations, and downregulations of genes directly or indirectly associated with riboflavin biosynthesis were often combined by manipulation with nutritional and other growth factors (Ledesma-Amaro et al, 2014, 2015Revuelta et al, 2016;Schwechheimer et al, 2016).…”

“…However, further studies on industrial riboflavin processes are needed to improve the main steps, such as fermentation conditions, purification, and availability to use recycled sources. Perhaps, usage of combined predictive models with advanced metabolic engineering techniques can help to optimize fermentation process and industrial strains for largescale production of vitamin B2 in the future (Wu et al, 2007;Suzuki et al, 2011;Oraei et al, 2018).…”

Valorization of residual yoghurt whey by lactic acid production: An optimized process

“…The significance of each coefficient was determined by student's t-test and p-values which are listed in Table 6. The larger the magnitude of the t-value and the smaller the p-value, the more significant is the corresponding coefficient [20,21]. The P-values were used as a tool to check the significance of each coefficient, which also indicates the interaction effects between each independent variable [22].…”

Isolamento e seleção de leveduras silvestres de biomas do Estado de São Paulo com potencial para produção de lipase e vitaminas do complexo B

“…Desta forma, uma sugestão para estudos futuros, seria ensaios com a redução deste componente, visando o aumento da produção de riboflavina extracelular, utilizando a linhagem Pichia caribbica.Em relação a produção de Biotina, o gênero Candida já foi estudado porHong et al. (2006) e por Suzuki;Macedo (2011), sendo alcançadas produções de Biotina extracelular de 1,8 mg/L e 8,3 µg/mL respectivamente. A linhagem isolada no presente trabalho RP.J1308 classificada como Candida oleophila, foi capaz de produzir 11,28 µg/mL demonstrando o seu potencial para a produção desta vitamina.…”