Synergistic growth effect among bacteria recovered from root canal infections

Júnior, Gil Moreira; Sobrinho, Antônio Paulino Ribeiro; Bambirra, Bernardo Henrique Silva; Bambirra, Felipe Henrique Silva; Carvalho, Maria Auxiliadora Roque de; Farias, Luiz M.; Nicoli, Jacques R.; Moreira, E. S. A.

doi:10.1590/s1517-83822011000300017

Cited by 5 publications

References 34 publications

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Unlocking the Mechanism of Action: A Cost-Effective Flow Cytometry Approach for Accelerating Antimicrobial Drug Development

Mermans,

Baets,

García-Timermans

et al. 2023

Preprint

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Antimicrobial resistance is one of the greatest challenges to global health. While the development of new antimicrobials can combat resistance, low profitability is reducing the number of new compounds brought to the market. Elucidating the mechanism of action is crucial for developing new antimicrobials. This process can become expensive as there are no universally applicable pipelines and scientific expertise in different fields is required. One way to determine the mechanism of action is the use of predictive modeling, as antimicrobials can be classified into limited groups.. We demonstrate a cost-effective flow cytometry approach for determining the mechanisms of action of new compounds. Cultures ofActinomyces viscosusandFusobacterium nucleatumwere treated with a range of antimicrobials and measured by flow cytometry. A Gaussian mixture mask was applied over the data to construct a phenotypic fingerprint. The fingerprints were used to train random forest classifiers, and classifiers were used to predict the mechanism of action of cephalothin. Significant statistical differences were found among the 10 different treatment groups. A pairwise comparison between treatment groups showed a statistical difference for 35 out of 45 pairs forActinomyces viscosusand 32 out of 45 pairs forFusobacterium nucleatumafter 3.5h of treatment. The best performing random forest classifier yielded a Matthews correlation coefficient of 0.92 and the mechanism of action of cephalothin could be successfully predicted. These findings suggest that flow cytometry can be a cheap and fast alternative for determining the mechanism of action of new antimicrobials.

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Unlocking the Mechanism of Action: A Cost-Effective Flow Cytometry Approach for Accelerating Antimicrobial Drug Development

Mermans,

Baets,

García-Timermans

et al. 2023

Preprint

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Microbiota Associated with Infections of the Jaws

Gaetti‐Jardim

Landucci

Oliveira

et al. 2012

International Journal of Dentistry

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The microbial infections involving the craniofacial skeleton, particularly maxilla and mandible, have direct relationship with the dental biofilm, with predominance of obligate anaerobes. In some patients, these infections may spread to bone marrow or facial soft tissues, producing severe and life-threatening septic conditions. In such cases, local treatment associated with systemic antimicrobials should be used in order to eradicate the sources of contamination. This paper discuss the possibility of spread of these infections and their clinical implications for dentistry, as well as their etiology and aspects related to microbial virulence and pathogenesis.

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