Taxonomic study and partial characterization of antimicrobial compounds from a moderately halophilic strain of the genus Actinoalloteichus

Abstract

A moderately halophilic actinomycete strain designated AH97 was isolated from a saline Saharan soil, and selected for its antimicrobial activities against bacteria and fungi. The AH97 strain was identified by morphological, chemotaxonomic and phylogenetic analyses to the genus Actinoalloteichus. Analysis of the 16S rDNA sequence of strain AH97 showed a similarity level ranging between 95.8% and 98.4% within Actinoalloteichus species, with A. hymeniacidonis the most closely related. The comparison of the physiological characteristics of AH97 with those of known species of Actinoalloteichus showed significant differences. Strain AH97 showed an antibacterial and antifungal activity against broad spectrum of microorganisms known to be human and plant pathogens. The bioactive compounds were extracted from the filtrate culture with n-butanol and purified using thin layer chromatography and high pressure liquid chromatography procedures. Two active products were isolated, one hydrophilic fraction (F1) and another hydrophobic (F2). Ultraviolet-visible, infrared, mass and 1H and 13C nuclear magnetic resonance spectroscopy studies suggested that these molecules were the dioctyl phthalate (F2) and an aminoglycosidic compound (F1).

Phylogenetic Analysis and Screening of Antimicrobial and Antiproliferative Activities of Culturable Bacteria Associated with the Ascidian Styela clava from the Yellow Sea, China

“…HQA030, belonging to the genus Actinoalloteichus , showed potent antimicrobial activity in our study. Two bioactive compounds, F1 (hydrophilic) and F2 (hydrophobic), were isolated from an Actinoalloteichus strain, which was isolated from a saline Saharan soil and showed antibacterial and antifungal activities against a broad spectrum of microorganisms known to be human and plant pathogens [91]. Further studies will be necessary to identify the bioactive substances produced by HQA030.…”

Antagonistic Properties of Some Halophilic Thermoactinomycetes Isolated from Superficial Sediment of a Solar Saltern and Production of Cyclic Antimicrobial Peptides by the Novel Isolate Paludifilum halophilum

“…Amplification was made using a Basic PCR protocol which consisted of an initial denaturation at 95°C for 10 min, followed by 30 amplification cycles of 94°C for 45 s, 52°C for 30 s, and 72°C for 1 min and 30 s and a final extension step of 72°C for 10 min [ 31 ]. The amplification result was detected by agarose gel (1%) electrophoresis and visualized by ultraviolet fluorescence after ethidium bromide staining [ 32 ]. The purification of DNA 16S fragment from PCR on agarose gels was performed using the PureLink Quick Gel Extraction and PCR Purification Combo Kit.…”

A new Streptomyces strain isolated from Saharan soil produces di-(2-ethylhexyl) phthalate, a metabolite active against methicillin-resistant Staphylococcus aureus

“…TN17 (Smaoui et al 2011) and S. mirabilis strain NSQu-25 (El-Sayed 2012), and also by Nocardia levis MK-VL_113 (Kavitha et al 2009), Actinoalloteichus sp. AH97 (Boudjelal et al 2011) and a brown algae Sargassum wightii (Sastry and Rao 1995). In addition to its antibacterial activity, it has been reported that di-(2-ethylhexyl) phthalate had other biological activities such as anti-leukemic and anti-mutagenic effects (Lee et al 2000).…”