New antimicrobial combinations: substituted chalcones- oxacillin against methicillin resistant Staphylococcus aureus

Talia, Juan Manuel; Debattista, Nora B.; Pappano, Nora B.

doi:10.1590/s1517-83822011000200010

Cited by 19 publications

(10 citation statements)

References 20 publications

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“…Investigation of in vitro synergism between several chalcones substituted in combination with oxacillin, an antibiotic used conventionally against S. aureus ATCC 43 300 that is resistant to meticillin, using the kinetic turbidimetric method developed earlier. The results were satisfactory for all assayed combinations and in accordance with the mechanism of bacteriostatic inhibition previously proposed, except for 2′,4′-dihydroxy-3′-methoxychalconeoxacillin (Talia et al 2011).…”

Section: Discussionsupporting

confidence: 88%

Antimicrobial and antioxidant activities of different extracts from Aspergillus unguis SPMD-EGY grown on different media

Hamed

Abdel‐Aziz

El-Hady³

2018

Bull Natl Res Cent

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Background: Recently, researchers demonstrate the importance of associated microorganisms as potential sources of bioactive natural products. Especially, marine-derived fungi consider a treasure of new pharmaceutical leads due to the chemical diversity of their secondary metabolites. Results: Aspergillus unguis SPMD-EGY was isolated from the soft coral Sinularia sp. and was cultivated on different media. Three broth media potato dextrose broth (PDB), peptone yeast extract malt extract glucose (PYMG), and Dox media in addition to one solid medium (rice medium) were used for this purpose. The fungus was grown under shake and static conditions (for broth media only). Ethyl acetate extract from both mycelia and culture supernatant and the extract from the direct extraction of rice solid medium were tested for their antimicrobial and antioxidant activities. It has been found that PYMG medium (mycelia static and culture static) exhibited the highest antimicrobial activity against all test microbes except Aspergillus niger followed by PDB medium (culture static and mycelia static). From the antioxidant point of view, the PDB medium showed the highest antioxidant activity (culture-shake) followed by Dox medium (culture static and mycelia static). The obtained results have been assessed and compared to the results of previous works constructed. GC/MS analysis of the fungal extracts showing high significant antimicrobial activities was evaluated. Conclusion: Three different culture media were tested for the cultivation of the locally isolated fungus Aspergillus unguis isolate SPMD-EGY and were tested as antimicrobial and antioxidant agents. It has been found that growth media and growth incubation conditions have a very important role in secondary metabolites production.

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Section: Discussionsupporting

confidence: 88%

Antimicrobial and antioxidant activities of different extracts from Aspergillus unguis SPMD-EGY grown on different media

Hamed

Abdel‐Aziz

El-Hady³

2018

Bull Natl Res Cent

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“…The emergence of a pathogen community depends on its ability to survive in different enviroments and to interact successfully with the host (18). As an additional stress, they may be exposed to a wide range of antimicrobially active agents, such as antibiotics, that can act as a selective pressure for the development of microorganisms resistance.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial activity of meta-alkoxyphenylcarbamates containing substituted N-phenylpiperazine fragment

Malík¹,

Bukovský²,

Andriamainty³

et al. 2012

Braz. J. Microbiol.

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In the present investigation, the basic esters of meta-alkoxyphenylcarbamic acid bearing variously substituted N-phenylpiperazine fragment were screened for their in vitro antimicrobial activity against Staphylococcus aureus, Escherichia coli and Candida albicans, respectively. The most effective againstEscherichia coli was found the compound 6d (MIC=195,3 μg/mL) bearing simultaneously para-fluoro substituent at the 4-phenylpiperazin-1-yl core and meta-methoxy side chain in the lipophilic part of the molecule. From whole analyzed set of the molecules the substance 8e with propoxy side chain forming meta-alkoxyphenylcarbamoyl fragment and lipophilic, sterically bulky meta-trifluoromethyl group attached at N-phenylpiperazine moiety was evaluated as the most active against Candida albicans (MIC=97,7 μg/mL). On the contrary, all investigated structures were practically inactive against Staphylococcus aureus (MIC>1000 μg/mL)

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“…Antistaphylococcal activity of various chalcones is strongly correlated with their chemical characteristics. It is generally considered that lipophilicity of the ring A (Nowakowska, 2007) and presence of free hydroxyl group/s at various positions of the ring B are necessary for antistaphylococcal activity of these compounds (Sato et al, 1996;Alcaraz et al, 2000;Kromann et al, 2004;Talia et al, 2011). Three compounds investigated in our study carrying free hydroxyl group at various positions of the B ring exerted strong anti-MRSA activity, with 1,3-Bis-(2-hydroxy-phenyl)-propenone (O-OH) bearing the hydroxyl group at the position 2 of ring B as the most active one.…”

Section: T H I S C O P Y I S F O R P E R S O N a L U S E O N L Y -D Imentioning

confidence: 99%

Inhibitory Effect of Newly-Synthesized Chalcones on Hemolytic Activity of Methicillin-Resistant Staphylococcus aureus

Božić¹,

Milenković²,

Ivković³

et al. 2015

Pol J Microbiol

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A b s t r a c tPathogenicity of methicillin-resistant Staphylococcus aureus (MRSA) is associated with a broad spectrum of virulence factors, amongst which is α-hemolysin. The aim of this study was to investigate the effect of three newly-synthesized chalcones (1,3-Bis-(2-hydroxy-phenyl)-propenone, 3-(3-Hydroxy-phenyl)-1-(2-hydroxy-phenyl)-propenone and 3-(4-Hydroxy-phenyl)-1-(2-hydroxy-phenyl)-propenone) on α-hemolysin production of clinical isolates of MRSA. Subinhibitory concentrations of the tested compounds reduced hemolytic activity of MRSA strains, with almost complete abolishment of hemolysis at concentrations in the range of 1/2-1/4 x MIC (25-12.5 μg/ml). In conclusion, newly-synthesized chalcones tested in this study showed potent inhibitory activity on α-hemolysin production of multiresistant and genetically diverse MRSA strains. 380auditory canal (1) and one laboratory control strain of methicillin-resistant S. aureus ATCC 43300 (KWIK-STIK TM , Microbiologics, USA). Identification of the isolates and methicillin resistance were determined by VITEK 2 test cards GP and AST-P580 (bioMérieux, France) and confirmed by PCR for nuc (Brakstad et al., 1992) and mecA (Bignardi et al., 1996) genes. Genotyping (SCCmec, agr, pvl and spa typing) of MRSA was performed according to previously described protocols (Boye et al., 2007;Lina et al., 2003;Harmsen et al., 2003). The spa types were clustered into spa clonal complexes (CCs) using the algorithm based upon repeat pattern (BURP) with Ridom Staph-Type 1.4 software (http://www.ridom.de). The multiresistance profile of MRSA strains was determined by VITEK 2 test card AST-P580 and further supplemented with disc diffusion test according to CLSI guidelines (CLSI, 2007). Chalcones tested in this study ( Fig. 1) Clinical isolates of MRSA were genetically heterogeneous and expressed multiresistance phenotype (Table I). MRSA strains were classified into SCCmec type I (55.5%), II (5.0%), III (20.0%), IV (10.0%) and V (10.0%) and agr type I (35.0%), II (60.0%) and III (5.0%). Strains belonged to 10 spa types and were clustered into 5 spa CCs, with most frequent CC5 (55.0%) and CC8 (20.0%).Tested chalcones exerted inhibitory activity against MRSA, with the order of potency of chalcones (average MIC ± SD) as following: O-OH (MIC = 37.5 ± 13.2 μg/ml) > M-OH (MIC = 97.5 ± 27.5 μg/ml) > P-OH (MIC = 110.8 ± 21.1 μg/ml). The most significant dosedependent inhibition of hemolysis was observed in MRSA supernatants cultivated with O-OH chalcone (Fig. 2). A 91.6-99.7% reduction of hemolysis was detected in all MRSA strains cultivated with ½ × MIC of O-OH. Hemolytic activity of MRSA strains cultivated with 1/2 × MIC (25.0 µg/ml) and 1/4 × MIC (12.5 µg/ml) of O-OH was in the range of 3.8-8.2% of the positive control (p < 0.001). Mild increase of the hemolytic activity was detected after cultivation with 1/8 × MIC (6.2 µg/ml) up to 19% (p < 0.01), and 1/16 × MIC (3.1 µg/ml) up to 34.6% (p < 0.01). The effect of M-OH 1 .2 µg/ml) did not reduce the hemolytic activity of MRSA (p > 0.0...

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New antimicrobial combinations: substituted chalcones- oxacillin against methicillin resistant Staphylococcus aureus

Cited by 19 publications

References 20 publications

Antimicrobial and antioxidant activities of different extracts from Aspergillus unguis SPMD-EGY grown on different media

Antimicrobial and antioxidant activities of different extracts from Aspergillus unguis SPMD-EGY grown on different media

Antimicrobial activity of meta-alkoxyphenylcarbamates containing substituted N-phenylpiperazine fragment

Inhibitory Effect of Newly-Synthesized Chalcones on Hemolytic Activity of Methicillin-Resistant Staphylococcus aureus

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