Detection of Bartonella henselae in defibrinated sheep blood used for culture media supplementation

Drummond, Marina Rovani; Pitassi, Luiza Helena Urso; Lania, Bruno Grosselli; Santos, Sílvio Rogério Cardozo dos; Gilioli, Rovílson; Velho, Paulo Eduardo Neves Ferreira

doi:10.1590/s1517-83822011000200003

Cited by 6 publications

(4 citation statements)

References 9 publications

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“…A negative control flask containing only BAPGM medium was added to each batch of samples tested and subjected to the same laboratory procedures and culture conditions. Subsequently, 1 mL of the blood-inoculated liquid culture was sub-inoculated onto agar enriched slant tubes containing 30% Bartonella spp.-negative sheep blood (confirmed by PCR and culture methods) [ 24 ] for additional 42 days. BAPGM-negative controls were also subcultured onto blood-agar.…”

Section: Methodsmentioning

confidence: 99%

Bartonella spp. Bacteremia in Blood Donors from Campinas, Brazil

et al. 2015

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Bartonella species are blood-borne, re-emerging organisms, capable of causing prolonged infection with diverse disease manifestations, from asymptomatic bacteremia to chronic debilitating disease and death. This pathogen can survive for over a month in stored blood. However, its prevalence among blood donors is unknown, and screening of blood supplies for this pathogen is not routinely performed. We investigated Bartonella spp. prevalence in 500 blood donors from Campinas, Brazil, based on a cross-sectional design. Blood samples were inoculated into an enrichment liquid growth medium and sub-inoculated onto blood agar. Liquid culture samples and Gram-negative isolates were tested using a genus specific ITS PCR with amplicons sequenced for species identification. Bartonella henselae and Bartonella quintana antibodies were assayed by indirect immunofluorescence. B. henselae was isolated from six donors (1.2%). Sixteen donors (3.2%) were Bartonella-PCR positive after culture in liquid or on solid media, with 15 donors infected with B. henselae and one donor infected with Bartonella clarridgeiae. Antibodies against B. henselae or B. quintana were found in 16% and 32% of 500 blood donors, respectively. Serology was not associated with infection, with only three of 16 Bartonella-infected subjects seropositive for B. henselae or B. quintana. Bartonella DNA was present in the bloodstream of approximately one out of 30 donors from a major blood bank in South America. Negative serology does not rule out Bartonella spp. infection in healthy subjects. Using a combination of liquid and solid cultures, PCR, and DNA sequencing, this study documents for the first time that Bartonella spp. bacteremia occurs in asymptomatic blood donors. Our findings support further evaluation of Bartonella spp. transmission which can occur through blood transfusions.

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Section: Methodsmentioning

confidence: 99%

Bartonella spp. Bacteremia in Blood Donors from Campinas, Brazil

et al. 2015

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“…After a 14-day incubation period, a 1-ml aliquot of liquid culture medium was used for DNA extraction and another 1-ml aliquot was plated onto a blood agar plate and incubated for an additional 42 days. Blood agar plates were prepared with 30% of sheep blood confirmed to be free of Bartonella DNA by PCR (2). The liquid culture negative-control blood-agar plate was incubated under the same conditions.…”

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Bartonella clarridgeiae Bacteremia Detected in an Asymptomatic Blood Donor

et al. 2015

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“…The pellets were then inoculated on BHI agar (Biolife, Ref. 4012352) containing 5% sheep blood that was confirmed to be Bartonella negative ( 20 , 21 ) and 2% Brucella growth supplement (HiMedia, FD005) and then incubated at 35°C in an atmosphere of 5% carbon dioxide (CO 2 ) for 4–8 weeks ( 18 , 22 ). Oral swabs and claw samples were enriched in 5 ml of BHI broth containing 5% sheep blood and Brucella growth supplement, before being incubated at 35°C in an atmosphere of 5% CO 2 for 10 days ( 21 , 23 , 24 ).…”

Section: Methodsmentioning

confidence: 99%

Serological and Molecular Detection of Bartonella henselae in Cats and Humans From Egypt: Current Status and Zoonotic Implications

et al. 2022

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Bartonellosis is a vector-borne zoonotic disease caused by the intracellular bacterium of genus Bartonella. The disease has a worldwide distribution and cats represent the major reservoir of this disease. Despite its global distribution, very limited previous studies have investigated the occurrence of bartonellosis in cats and their owners in Egypt. In an endeavor to explore this topic, we investigated the occurrence of Bartonella henselae (B. henselae) infection in 225 samples (blood, saliva, and claw) obtained from 75 healthy cats in Upper Egypt. These samples were routinely obtained during veterinary clinic visits. This study also involved an examination of 100 humans, including cat owners and people with a history of contact with cats. Attempted isolation and identification of B. henselae in cats were also performed. Furthermore, PCR was performed for molecular identification of B. henselae in blood samples from cats. Meanwhile, an immunofluorescent assay was performed to study the seroprevalence of B. henselae infection in humans. In this study, B. henselae could not be isolated from any of the examined blood, saliva, or claw samples from cats. Interestingly, B. henselae was identified molecularly in 8% (6/75) of blood samples from cats. The seroprevalence of B. henselae in humans was 46% and its occurrence was higher in females (46.6%) than in males (41.7%) (P = 0.748). B. henselae infection was higher among cat owners [51.4% (19/37)] than among people with a history of contact with cats [42.9% (27/63)] (P = 0.410). Infection was higher in rural regions [79.5% (31/39)] than in urban regions [24.6% (15/61)] (P < 0.001). Collectively, this data provide interesting baseline information about the occurrence of B. henselae in cats and humans in Upper Egypt, which reflects the potential zoonotic transmission of this bacterium. Future study is mandatory to explore the occurrence of B. henselae in major reservoirs in Egypt.

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Detection of Bartonella henselae in defibrinated sheep blood used for culture media supplementation

Cited by 6 publications

References 9 publications

Bartonella spp. Bacteremia in Blood Donors from Campinas, Brazil

Bartonella spp. Bacteremia in Blood Donors from Campinas, Brazil

Bartonella clarridgeiae Bacteremia Detected in an Asymptomatic Blood Donor

Serological and Molecular Detection of Bartonella henselae in Cats and Humans From Egypt: Current Status and Zoonotic Implications

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