Comparative analysis of two component signal transduction systems of the Lactobacillus acidophilus group

Abstract

The Lactobacillus acidophilus group is a phylogenetically distinct group of closely related lactobacilli. Members of this group are considered to have probiotic properties and occupy different environmental niches. Bacteria generally sense and respond to environmental changes through two component systems (TCSs) which consist of a histidine protein kinase (HPK) and its cognate response regulator (RR). With the use of in silico techniques, the five completely sequenced L. acidophilus group genomes were scanned in order to predict TCSs. Five to nine putative TCSs encoding genes were detected in individual genomes of the L. acidophilus group. The L. acidophilus group HPKs and RRs were classified into subfamilies using the Grebe and Stock classification method. Putative TCSs were analyzed with respect to conserved domains to predict biological functions. Putative biological functions were predicted for the L. acidophilus group HPKs and RRs by comparing them with those of other microorganisms. Some of TCSs were putatively involved in a wide variety of functions which are related with probiotic ability, including tolerance to acid and bile, production of antimicrobial peptides, resistibility to the glycopeptide antibiotic vancomycin, and oxidative condition.

Comparative Genomics and Specific Functional Characteristics Analysis of Lactobacillus acidophilus

“…In this study, predictions of antibiotic resistance genes have been made for each strain. Additionally, L. acidophilus has the most resistance genes of fluoroquinolone, glycopeptide, lincosamide, macrolide and tetracycline, which was consistent with previous reports [ 52 , 53 , 54 , 55 ].…”

Complete Genome Sequencing and Comparative Genomics of Three Potential Probiotic Strains, Lacticaseibacillus casei FBL6, Lacticaseibacillus chiayiensis FBL7, and Lacticaseibacillus zeae FBL8

“…For lactic acid bacteria to survive while passing through the gastrointestinal tract, they need to have the ability to sense and respond to conditions in various changing environments (Zhang et al, 2019). They are generally detected by a two-component system (Cui and Qu, 2011), which consists of a membrane-associated histidine kinase that detects specific environmental signals and a cytoplasmic response regulator regulating gene expression (Cui and Qu, 2011). FBL8 carried a distinguished two-component system sensor histidine kinase.…”

Characterization of Two Component Systems of Lactobacillus casei BL23 and their involvement in stress response

“…Different studies have addressed the role of TCS in the biology and stress response in Lactobacillus acidophilus Cui and Qu, 2011), Lactobacillus plantarum Sturme et al, 2005;Maldonado-Barragán et al, 2009), Lactobacillus sakei and Lactobacillus delbrueckii . However, no studies on TCS in Lactobacillus casei had been reported when this PhD thesis started.…”