Evaluating most probable number method to count and isolate viable methylotrophs

Kashyap, Sudhanshu

doi:10.1590/s1517-83822011000100006

Cited by 2 publications

(2 citation statements)

References 6 publications

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“…In this way, the modified MPN drop agar method provides significant benefit compared to current state of art [11,[22][23][24][25], compared especially with drop agar methods cited in the literature. There, 96-well tissue plate was obviously used for dilution preparation and drops with dilutions were inoculated to one plate [26][27][28].…”

Section: Resultsmentioning

confidence: 99%

MPN Drop Agar Method for Determination of Heterotrophic Microorganisms in Soil and Water Samples Using Tissue Plate as a Carrier

Chumchalová

Kubal

2020

Sustainability

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The most probable number (MPN) method is a culture-based method commonly used in the field of environmental microbiology to examine microbial populations in liquid substrates. The MPN tests require a wide range of special laboratory equipment, a lot of laboratory space, and skilled staff, which together limit their applicability. This paper presents a modified MPN method, which reduces the experimental requirements by applying tissue plate as a carrier. The modified MPN method introduces a fast-filled tissue plate with 5 × 5 squares as agar carrier, instead of the commonly used set of glass tubes. Further, self-refilling automatic syringe Socorex® was implemented to apply samples to the plate. The response of the modified MPN method was tested on eight selected bacterial strains as well as on soil and water samples. Simultaneously, all the strains and samples were tested by standard spread plate method. High linear correlation between the two methods was found, which makes a new modified MPN method a useful alternative within the field of environmental microbiology.

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Section: Resultsmentioning

confidence: 99%

MPN Drop Agar Method for Determination of Heterotrophic Microorganisms in Soil and Water Samples Using Tissue Plate as a Carrier

Chumchalová

Kubal

2020

Sustainability

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“…The MPN method is one of the primary methods for the enumeration of L. monocytogenes and other foodborne pathogens, and is supported by enrichment schemes that can potentially suppress the level of background flora (Capita and Alonso-Calleja, 2003;Chen et al, 2017). However, the MPN method is laborious and requires the use of a large amount of media (Sutton, 2010;Kashyap, 2011;Erkmen and Bozoglu, 2016). Furthermore, the MPN method cannot be applied for a large number of samples simultaneously, making it difficult to utilize the data for constructing a growth curve under various conditions (Kawasaki et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

A comparison of <i>Listeria monocytogenes</i> growth monitoring in ground pork samples by real-time polymerase chain reaction to conventional agar and most probable number methods

Noviyanti

Hosotani

Inatsu

et al. 2021

FSTR

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To construct a predictive growth model of Listeria monocytogenes in ground pork, growth data were collected by real-time PCR and Listeria selective agar according to Ottaviani and Agosti (ALOA). Most probable number (MPN) was used to estimate viable numbers of L. monocytogenes at the beginning, middle, and end of the incubation period. Growth curves obtained from this study were fitted to the Baranyi and Roberts model to obtain growth parameters. Furthermore, the theoretical minimum temperature of growth was estimated by Ratkowsky's model. L. monocytogenes growth rates estimated from ALOA data were lower than those estimated by real-time PCR. Moreover, cell concentrations at all incubation temperatures were underestimated and lag phase duration at refrigeration temperature (4 °C) was overestimated by ALOA. However, the estimation from MPN more closely resembled the real-time PCR quantification results. Thus, the direct plate count tends to result in fail-dangerous prediction for bacterial risk from the model.

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Evaluating most probable number method to count and isolate viable methylotrophs

Abstract: Nine different receptacles were tested with the MPN method to determine which receptacle was most reliable and economical for MPN counts. Results showed that 96 well PCR plate were the best vessels for this type of analysis and facilitated the isolation of viable Methylotrophs.

Cited by 2 publications

References 6 publications

MPN Drop Agar Method for Determination of Heterotrophic Microorganisms in Soil and Water Samples Using Tissue Plate as a Carrier

MPN Drop Agar Method for Determination of Heterotrophic Microorganisms in Soil and Water Samples Using Tissue Plate as a Carrier

A comparison of <i>Listeria monocytogenes</i> growth monitoring in ground pork samples by real-time polymerase chain reaction to conventional agar and most probable number methods

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