Quantitative changes in the biochemical composition of lignocellulosic residues during the vegetative growth of Lentinula edodes

Gaitán-Hernández, Rigoberto; Esqueda, Martín; Gutiérrez, Aldo; Beltrán-García, Miguel J.

doi:10.1590/s1517-83822011000100004

Cited by 36 publications

(27 citation statements)

References 33 publications

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“…Indeed, L . edodes (Gaitán‐Hernández et al ., ; Cai et al ., ) and P . ostreatus (Isikhuemhen and Mikiashvilli, ) have been reported to produce high levels of laccase and Mn peroxidase activities thereby degrading lignin as one of their major C sources.…”

Section: Discussionmentioning

confidence: 98%

Multi‐omic analyses of exogenous nutrient bag decomposition by the black morel Morchella importuna reveal sustained carbon acquisition and transferring

Tan

Kohler

Miao

et al. 2019

Environmental Microbiology

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Summary The black morel (Morchella importuna Kuo, O'Donnell and Volk) was once an uncultivable wild mushroom, until the development of exogenous nutrient bag (ENB), making its agricultural production quite feasible and stable. To date, how the nutritional acquisition of the morel mycelium is fulfilled to trigger its fruiting remains unknown. To investigate the mechanisms involved in ENB decomposition, the genome of a cultivable morel strain (M. importuna SCYDJ1‐A1) was sequenced and the genes coding for the decay apparatus were identified. Expression of the encoded carbohydrate‐active enzymes (CAZymes) was then analyzed by metatranscriptomics and metaproteomics in combination with biochemical assays. The results show that a diverse set of hydrolytic and redox CAZymes secreted by the morel mycelium is the main force driving the substrate decomposition. Plant polysaccharides such as starch and cellulose present in ENB substrate (wheat grains plus rice husks) were rapidly degraded, whereas triglycerides were accumulated initially and consumed later. ENB decomposition led to a rapid increase in the organic carbon content in the surface soil of the mushroom bed, which was thereafter consumed during morel fruiting. In contrast to the high carbon consumption, no significant acquisition of nitrogen was observed. Our findings contribute to an increasingly detailed portrait of molecular features triggering morel fruiting.

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Section: Discussionmentioning

confidence: 98%

Multi‐omic analyses of exogenous nutrient bag decomposition by the black morel Morchella importuna reveal sustained carbon acquisition and transferring

Tan

Kohler

Miao

et al. 2019

Environmental Microbiology

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“…Various techniques have been applied to improve the nutritional value of these fibrous foods, including physical, chemical and biological treatments (Liu et al, 1999;Tuyen et al, 2012). The biological methods include those based on the use of hydrolytic enzymes and microorganisms, particularly saprophytic fungi with fibrolytic potential (Zafar et al, 1996;Kuhad et al, 1997;Rodrigues et al, 2008;Gaitán-Hernández et al, 2011). These biological methods can improve the nutritional quality of fibrous foods by increasing true protein (TP) content (Velásquez et al, 2012), and improve digestibility by decreasing cell wall content (Kuhad et al, 1997;Villas-Boas et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Improvement in nutritional quality of fibrous food via in vitro digestion by Aspergillus niger

Velásquez

Marnet²,

Árias

2015

Cienc. Inv. Agr.

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A. Velásquez, P. Marnet, and R. Arias. 2015. Improvement in nutritional quality of fibrous food via in vitro digestion by Aspergillus niger. Cien. Inv. Agr. 42(1): 45-55. In a first experiment, the effect of in vitro incubation with Aspergillus niger (An) on the chemical composition of different fibrous substrates was studied. In a second experiment, the effect of incubation time (0, 72 and 144 h) on in vitro digestion with An of dry matter (IVDMD), neutral detergent fiber (DVNDF) and acid detergent fiber (DVADF) was evaluated for different substrates. Wheat straw, WS; barley straw, BS; oat hulls, OH; dehydrated alfalfa, DA; and dehydrated ryegrass, DB substrates were evaluated. In both experiments, incubations were performed in 250 mL Erlenmeyer flasks; 2 g of substrate was added to a culture medium (pH = 6), and incubated at 28 °C with constant ventilation. The An dose consisted of 3 mL of a solution of 5.3 × 10 6 spores mL -1 per flask. Incubation time for the first experiment was 144 h. After incubation, the contents of the flasks were homogenized in a blender for one minute. Subsequently, bromatological analysis was conducted without separating the An biomass. No interaction effects among any studied variables were observed. An incubation effected cell wall (NDF) and lignocellulose (ADF) content. An increase in true protein (TP) and soluble nitrogen (SN) was observed for all substrates tested, but responses differed depending on the type of substrate. The largest increases in TP were observed in DA and DB substrates (0.55 and 0.63% DM, respectively). IVDMD, DVNDF and DVADF were affected by the type of substrate and incubation time. The highest value of IVDMD 144 was observed in the DAAn treatment (49.47% DM), followed by the DBAn treatment, with a value of 45.51% DM. OHAn showed the lowest value of IVDMD 144 (29.38% DM). The results suggest that An possesses fibrolytic and metabolic potential for improving the nutritional value of fibrous foods through of the digestion of structural carbohydrates and the liberation of nitrogenous fractions embedded in cell walls, producing a change in the chemical composition and the potential digestibility of treated foods.

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“…Alnus, Bursera, Carpinus, Eucalyptus, Heliocarpus and Nothofagus (Mata et al , 1990; Morales and Martínez-Carrera, 1991; Morales et al , 1991; Pire et al , 2001). Coffee pulp, sugar cane bagasse, straw from several cereals and vineyard pruning have also been employed (Mata and Gaitán-Hernández, 1992, 1994; Salmones et al , 1999; Gaitán-Hernández and Mata, 2004; Gaitán-Hernández et al , 2006; Gaitán-Hernández et al , 2011). Land cultivated with barley and wheat crops in Mexico cover from 383,000 to 560,000 ha, generating approximately 0.7 and 2.7 million tons of straw residue, respectively (FAO, 2009).…”

Section: Introductionmentioning

confidence: 99%

“…Barley and wheat are widely used in the cultivation of Agaricus bisporus (Lange) Imbach and Pleurotus spp . , they also provide a great potential for the production of shiitake (Gaitán-Hernández and Mata, 2004; Gaitán-Hernández et al , 2006; Gaitán-Hernández et al , 2011). …”

Section: Introductionmentioning

confidence: 99%

Improvement of yield of the edible and medicinal mushroom Lentinula edodes on wheat straw by use of supplemented spawn

Gaitán-Hernández¹,

Cortés²

2014

Braz. J. Microbiol.

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The research evaluated the interactions of two main factors (strain / types of spawn) on various parameters with the purpose to assess its effect on yield and biochemical composition of Lentinula edodes fruiting bodies cultivated on pasteurized wheat straw. The evaluation was made with four strains (IE-40, IE-105, IE-124 and IE-256). Different types of spawns were prepared: Control (C) (millet seed, 100%), F1 (millet seed, 88.5%; wheat bran, 8.8%; peat moss, 1.3%; and CaS04, 1.3%) and F2 (the same formula as F1, but substituting the wheat bran with powdered wheat straw). Wheat straw was pasteurized by soaking it for 1 h in water heated to 65 °C. After this the substrate (2 kg wet weight) was placed in polypropylene bags. The bags were inoculated with each spawn (5% w/w) and incubated in a dark room at 25 °C. A proximate analysis of mature fruiting bodies was conducted. The mean Biological Efficiency (BE) varied between 66.0% (C-IE-256) and 320.1% (F1-IE-124), with an average per strain of 125.6%. The highest mean BE was observed on spawn F1 (188.3%), significantly different from C and F2. The protein content of fruiting bodies was high, particularly in strain IE-40-F1 (17.7%). The amount of fat varied from 1.1 (F1-IE-40) to 2.1% (F2-IE-105) on dry matter. Carbohydrates ranged from 58.8% (F1-IE-40) to 66.1% (F1-IE-256). The energy value determined ranged from 302.9 kcal (F1-IE-40) to 332.0 kcal (F1-IE-256). The variability on BE observed in this study was significantly influenced by the spawn’s formulation and genetic factors of the different strains.

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Quantitative changes in the biochemical composition of lignocellulosic residues during the vegetative growth of Lentinula edodes

Cited by 36 publications

References 33 publications

Multi‐omic analyses of exogenous nutrient bag decomposition by the black morel Morchella importuna reveal sustained carbon acquisition and transferring

Multi‐omic analyses of exogenous nutrient bag decomposition by the black morel Morchella importuna reveal sustained carbon acquisition and transferring

Improvement in nutritional quality of fibrous food via in vitro digestion by Aspergillus niger

Improvement of yield of the edible and medicinal mushroom Lentinula edodes on wheat straw by use of supplemented spawn

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