Some optimal culture conditions for production of cyclosporin a by Fusarium roseum

Ismaiel, Ahmed A.; El-Sayed, El-Sayed A.; Mahmoud, Amira A.

doi:10.1590/s1517-83822010000400033

Cited by 10 publications

(6 citation statements)

References 31 publications

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“…It was reported that Fusarium sp. produced cyclosporin A [71,72] and acts as an antifungal agent against other strains of fungi, such as some Aspergillus , Curvularia , Trichophyton , Rhodotorula , Botrytis , Alternaria , and Neurospora [72,73] . Cyclosporin A binds to the intracellular cyclophilin receptor to form a specific complex and then binds to calcineurin, which is a protein phosphatase dependent on calcium and calmodulin, inhibiting its enzymatic activity.…”

Section: Resultsmentioning

confidence: 99%

Dynamics of Chemical Diversity during Co‐Cultures: An Integrative Time‐Scale Metabolomics Study of Fungal Endophytes Cophinforma mamane and Fusarium solani

Triastuti

Haddad

Barakat

et al. 2021

Chemistry & Biodiversity

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A rapid and efficient metabolomic study of Cophinforma mamane and Fusarium solani co‐cultivation in time‐series based analysis was developed to study metabolome variations during their fungal interactions. The fungal metabolomes were studied through the integration of four metabolomic tools: MS‐DIAL, a chromatographic deconvolution of liquid‐chromatography‐mass spectrometry (LC/MS); MS‐FINDER, a structure‐elucidation program with a wide range metabolome database; GNPS, an effective method to organize MS/MS fragmentation spectra, and MetaboAnalyst, a comprehensive web application for metabolomic data analysis and interpretation. Co‐cultures of C. mamane and F. solani induced different patterns of metabolite production over 10 days of incubation and induced production of five de novo compounds not occurring in monocultures. These results emphasize that co‐culture in time‐frame analysis is an interesting method to unravel hidden metabolome in the investigation of fungal chemodiversity.

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Section: Resultsmentioning

confidence: 99%

Dynamics of Chemical Diversity during Co‐Cultures: An Integrative Time‐Scale Metabolomics Study of Fungal Endophytes Cophinforma mamane and Fusarium solani

Triastuti

Haddad

Barakat

et al. 2021

Chemistry & Biodiversity

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“…The irradiation presents an additional stress to the cells which tends to disturb their organization. Irradiation effects have been shown to occur with proteins, enzymes, nucleic acid, lipids and carbohydrates, all of which may have marked effects on the cell (Ismail et al, 2010).…”

Section: Resultsmentioning

confidence: 99%

Comparison of Free and Immobilized L-asparaginase Synthesized by Gamma-Irradiated Penicillium cyclopium

El-Refai¹,

Shafei²,

Mostafa³

et al. 2016

Pol J Microbiol

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A b s t r a c tGamma irradiation is used on Penicillium cyclopium in order to obtain mutant cells of high L-asparaginase productivity. Using gamma irradiation dose of 4 KGy, P. cyclopium cells yielded L-asparaginase with extracellular enzyme activity of 210.8 ± 3 U/ml, and specific activity of 752.5 ± 1.5 U/mg protein, which are 1.75 and 1.53 times, respectively, the activity of the wild strain. The enzyme was partially purified by 40-60% acetone precipitation. L-asparaginase was immobilized onto Amberlite IR-120 by ionic binding. Both free and immobilized enzymes exhibited maximum activity at pH 8 and 40°C. The immobilization process improved the enzyme thermal stability significantly. The immobilized enzyme remained 100% active at temperatures up to 60°C, while the free asparaginase was less tolerant to high temperatures. The immobilized enzyme was more stable at pH 9.0 for 50 min, retaining 70% of its relative activity. The maximum reaction rate (V max ) and Michaelis-Menten constant (K m ) of the free form were significantly changed after immobilization. The K m value for immobilized L-asparaginase was about 1.3 times higher than that of free enzyme. The ions K + , Ba 2+ and Na + showed stimulatory effect on enzyme activity with percentages of 110%, 109% and 106% respectively. K e y w o r d s: Penicillium cyclopium, Amberlite IR-120, gamma irradiation, ionic binding immobilization, L-asparaginase

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“…Fungi have a specific response to different growing environments that affects the biosynthesis of active secondary metabolites. Environmental changes can alter the chemical and biological profiles leading to metabolite diversification and, consequently, to novel pharmacological applications (Bracarense and Takahashi 2014; Ismaiel et al 2010).…”

Section: Discussionmentioning

confidence: 99%

“…Fungal endophytes are hyper-diverse and abundant groups that can be found colonizing the tissues of all plants, regardless of their taxonomical affiliation or environmental preferences (Ismaiel et al 2010). Throughout centuries, medicinal plants have been used as a source of molecules with therapeutic potential, and until today they represent an important pool for the identification of novel drug leads (Atanasov et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Bioprospecting of Diaporthe terebinthifolii LGMF907 for antimicrobial compounds

et al. 2018

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Antibiotic-resistant bacteria have been observed with increasing frequency over the past decades, driving the search for new drugs and stimulating the interest in natural products sources. Endophytic fungi from medicinal plants represent a great source of novel bioactive compounds useful to pharmaceutical and agronomical purposes. Diaporthe terebinthifolii is an endophytic species isolated from Schinus terebinthifolius, a plant used in popular medicine for several health problems. The strain D. terebinthifolii LGMF907 was previously reported by our group to produce secondary metabolites with biological activity against phytopathogens. Based on these data, strain LGMF907 was chosen for bioprospecting against microorganisms of clinical importance and for characterization of major secondary metabolites. In this study, different culture conditions were evaluated and the biological activity of this strain was expanded. The crude extracts demonstrated high antibacterial activity against Escherichia coli, Micrococcus luteus, Saccharomyces cerevisiae, methicillin-sensitive Staphylococcus aureus, and methicillin-resistant S. aureus. The compounds diaporthin and orthosporin were characterized and also showed activity against the clinical microorganisms evaluated. This study discloses the first isolation of diaporthin and orthosporin from D. terebinthifolii, and revealed the potential of this endophytic fungus to produce secondary metabolites with antimicrobial activity.

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Some optimal culture conditions for production of cyclosporin a by Fusarium roseum

Cited by 10 publications

References 31 publications

Dynamics of Chemical Diversity during Co‐Cultures: An Integrative Time‐Scale Metabolomics Study of Fungal Endophytes Cophinforma mamane and Fusarium solani

Dynamics of Chemical Diversity during Co‐Cultures: An Integrative Time‐Scale Metabolomics Study of Fungal Endophytes Cophinforma mamane and Fusarium solani

Comparison of Free and Immobilized L-asparaginase Synthesized by Gamma-Irradiated Penicillium cyclopium

Bioprospecting of Diaporthe terebinthifolii LGMF907 for antimicrobial compounds

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